Immobilization of lipases on glyoxyl-octyl supports: Improved stability and reactivation strategies

• Published in: Process Biochemistry
• Main Authors: Suescun, Angélica, Rueda, Nazzoly, Sousa dos Santos, José Cleiton, Castillo, John J., Ortiz, Claudia, Torres Sáez, Rodrigo, Barbosa, Oveimar, Fernández-Lafuente, Roberto
• Other Authors: Ministerio de Economía y Competitividad (España), Colciencias (Colombia), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier 2015
• Subjects: Enfolding/refolding; Covalent immobilization; Enzyme reactivation; Enzyme stabilization; Immobilization of lipases via interfacial activation; Rugosa; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10261/190168; https://doi.org/10.1016/j.procbio.2015.05.010; https://doi.org/10.13039/501100003593; https://doi.org/10.13039/501100003329

Lipases from Candida rugosa (CRL) and from Candida antarctica (isoform A) (CALA) have been successfully immobilized on octyl–glyoxyl agarose (OCGLX) beads and compared to the octyl–agarose (OC) or glyoxyl (GLX) beads immobilized counterparts. Immobilization on OCGLX gave similar hyperactivations tha...