Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases
Although reactions of transesterification are generally catalyzed by one specific lipase preparation, the concept of “combi-lipase” could be better explored for the production of biodiesel, since oils are heterogeneous substrates. In this research, we tested this concept by evaluating the enzymatic...
Published in: | Applied Catalysis A: General |
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ftcsic:oai:digital.csic.es:10261/190033 2024-02-11T09:55:53+01:00 Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases Poppe, Jakeline K. Matte, Carla R. Ruaro Peralba, Maria do Carmo Fernández-Lafuente, Roberto Rodrigues, Rafael C. Záchia Ayub, Marco A. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) Ministerio de Economía y Competitividad (España) 2015-01-25 http://hdl.handle.net/10261/190033 https://doi.org/10.1016/j.apcata.2014.10.050 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100002322 unknown Elsevier #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-41507-R https://doi.org/10.1016/j.apcata.2014.10.050 Sí Applied Catalysis A: General 490: 50-56 (2015) 0926-860X http://hdl.handle.net/10261/190033 doi:10.1016/j.apcata.2014.10.050 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002322 none Biodiesel Thermomyces lanuginosus lipase Rhizomucor miehei lipase Combilipases Enzyme reuse Candida antarctica lipase B artículo http://purl.org/coar/resource_type/c_6501 2015 ftcsic https://doi.org/10.1016/j.apcata.2014.10.05010.13039/50110000332910.13039/501100002322 2024-01-16T10:43:21Z Although reactions of transesterification are generally catalyzed by one specific lipase preparation, the concept of “combi-lipase” could be better explored for the production of biodiesel, since oils are heterogeneous substrates. In this research, we tested this concept by evaluating the enzymatic transesterification of olive and palm oils, two diverse fatty acid compositions, using standalone or mixtures of three immobilized lipases as biocatalysts: Novozym 435 (CALB), Lipozyme TL-IM (TLL), and Lipozyme RM-IM (RML). For olive oil, the combination of 29.0% of TLL, 12.5% of RML, and 58.5% of CALB was the best, allowing for 95% conversion efficiency in 18 h of reaction, up from 50% for the best individual lipase (CALB). For palm oil, the best enzyme combination was 52.5% of TLL and 47.5% of RML, resulting in 80% of conversion of ethyl esters in 18 h, compared to only 44% when standalone TLL was used. Repeated batches of reaction were carried out in order to test the operational stability of the combi-lipase systems, with results showing that they could be used for at least seven cycles keeping higher than 80% of their initial activities. This work was supported by grants from Brazilian Coordenação de Aperfoiçoamento de Pessoal de Nível Superior (CAPES) and CTQ2013-41507-R from Spanish MINECO. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Applied Catalysis A: General 490 50 56 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Biodiesel Thermomyces lanuginosus lipase Rhizomucor miehei lipase Combilipases Enzyme reuse Candida antarctica lipase B |
spellingShingle |
Biodiesel Thermomyces lanuginosus lipase Rhizomucor miehei lipase Combilipases Enzyme reuse Candida antarctica lipase B Poppe, Jakeline K. Matte, Carla R. Ruaro Peralba, Maria do Carmo Fernández-Lafuente, Roberto Rodrigues, Rafael C. Záchia Ayub, Marco A. Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
topic_facet |
Biodiesel Thermomyces lanuginosus lipase Rhizomucor miehei lipase Combilipases Enzyme reuse Candida antarctica lipase B |
description |
Although reactions of transesterification are generally catalyzed by one specific lipase preparation, the concept of “combi-lipase” could be better explored for the production of biodiesel, since oils are heterogeneous substrates. In this research, we tested this concept by evaluating the enzymatic transesterification of olive and palm oils, two diverse fatty acid compositions, using standalone or mixtures of three immobilized lipases as biocatalysts: Novozym 435 (CALB), Lipozyme TL-IM (TLL), and Lipozyme RM-IM (RML). For olive oil, the combination of 29.0% of TLL, 12.5% of RML, and 58.5% of CALB was the best, allowing for 95% conversion efficiency in 18 h of reaction, up from 50% for the best individual lipase (CALB). For palm oil, the best enzyme combination was 52.5% of TLL and 47.5% of RML, resulting in 80% of conversion of ethyl esters in 18 h, compared to only 44% when standalone TLL was used. Repeated batches of reaction were carried out in order to test the operational stability of the combi-lipase systems, with results showing that they could be used for at least seven cycles keeping higher than 80% of their initial activities. This work was supported by grants from Brazilian Coordenação de Aperfoiçoamento de Pessoal de Nível Superior (CAPES) and CTQ2013-41507-R from Spanish MINECO. Peer Reviewed |
author2 |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) Ministerio de Economía y Competitividad (España) |
format |
Article in Journal/Newspaper |
author |
Poppe, Jakeline K. Matte, Carla R. Ruaro Peralba, Maria do Carmo Fernández-Lafuente, Roberto Rodrigues, Rafael C. Záchia Ayub, Marco A. |
author_facet |
Poppe, Jakeline K. Matte, Carla R. Ruaro Peralba, Maria do Carmo Fernández-Lafuente, Roberto Rodrigues, Rafael C. Záchia Ayub, Marco A. |
author_sort |
Poppe, Jakeline K. |
title |
Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
title_short |
Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
title_full |
Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
title_fullStr |
Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
title_full_unstemmed |
Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
title_sort |
optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/10261/190033 https://doi.org/10.1016/j.apcata.2014.10.050 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100002322 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2013-41507-R https://doi.org/10.1016/j.apcata.2014.10.050 Sí Applied Catalysis A: General 490: 50-56 (2015) 0926-860X http://hdl.handle.net/10261/190033 doi:10.1016/j.apcata.2014.10.050 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002322 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.apcata.2014.10.05010.13039/50110000332910.13039/501100002322 |
container_title |
Applied Catalysis A: General |
container_volume |
490 |
container_start_page |
50 |
op_container_end_page |
56 |
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1790599311987310592 |