Chemical amination of lipases improves their immobilization on octyl-glyoxyl agarose beads

• Published in: Catalysis Today
• Main Authors: Rueda, Nazzoly, Dos Santos, José C. S., Ortiz, Claudia, Barbosa, Oveimar, Fernández-Lafuente, Roberto, Torres Sáez, Rodrigo
• Other Authors: Ministerio de Economía y Competitividad (España), Colciencias (Colombia), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier 2016
• Subjects: Hydrophobic supports; Chemical amination; Lipase stabilization; Covalent attachment; Interfacial activation of lipases; Martínez; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10261/189861; https://doi.org/10.1016/j.cattod.2015.05.027; https://doi.org/10.13039/501100003593; https://doi.org/10.13039/501100003329

This paper describes a new strategy that permits to take full advantage of octyl-agarose supports to immobilize lipases (one-step purification and immobilization, stabilization of the open form of the enzyme) but that may be used in any reaction media. To this purpose, we have utilized aminated lipa...