Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for deriv...
Published in: | International Journal of Molecular Sciences |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2019
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Subjects: | |
Online Access: | http://hdl.handle.net/10261/188976 https://doi.org/10.3390/ijms20164018 https://doi.org/10.13039/501100003593 https://doi.org/10.13039/501100002322 https://doi.org/10.13039/501100005283 https://doi.org/10.13039/501100003329 |
Summary: | In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for derivative activity). CALA-MNP biocatalyst was characterized by X-ray Powder Diffraction (XRPD), Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetry (TG) and Scanning Electron Microscope (SEM), proving the incorporation of magnetite and the immobilization of CALA in the chitosan matrix. Besides, the immobilized biocatalyst showed a half-life 8–11 times higher than that of the soluble enzyme at pH 5–9. CALA showed the highest activity at pH 7, while CALA-MNP presented the highest activity at pH 10. The immobilized enzyme was more active than the free enzyme at all studied pH values, except pH 7. This research was funded by Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), grant numbers BP3-0139-00005.01.00/18 and PNE-0112-00048.01.00/16, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), grant numbers 422942/2016-2, 409058/2015-5 and 408790/2016-4, Coordenação de Aperfeiçoamento de Ensino Superior (CAPES-Finance Code 001) and MICIU, grant number CTQ2015-68951-C3-3-R. Peer reviewed |
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