Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks
9 pages, and figures, and tables statistics The precise identification of the cyanobacteria that comprise an endolithic biofilm is hindered by difficulties in culturing the organisms found in these biofilms and a lack of previous molecular and ultrastructural data. This study characterizes, both at...
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2007
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Online Access: | http://hdl.handle.net/10261/18369 https://doi.org/10.1111/j.1574-6941.2006.00256.x |
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ftcsic:oai:digital.csic.es:10261/18369 2024-02-11T09:57:40+01:00 Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks Ríos, Asunción de los Grube, M. Sancho, Leopoldo G. Ascaso, Carmen 2007 22195 bytes application/pdf http://hdl.handle.net/10261/18369 https://doi.org/10.1111/j.1574-6941.2006.00256.x en eng Blackwell Publishing http://dx.doi.org/10.1111/j.1574-6941.2006.00256.x FEMS Microbiology ecology 59: 386-395 (2007) 1574-6941 http://hdl.handle.net/10261/18369 doi:10.1111/j.1574-6941.2006.00256.x none Antarctica Cyanobacteria 16S rRNA Endolithic biofilms Extracellular polymeric substances artículo http://purl.org/coar/resource_type/c_6501 2007 ftcsic https://doi.org/10.1111/j.1574-6941.2006.00256.x 2024-01-16T09:24:17Z 9 pages, and figures, and tables statistics The precise identification of the cyanobacteria that comprise an endolithic biofilm is hindered by difficulties in culturing the organisms found in these biofilms and a lack of previous molecular and ultrastructural data. This study characterizes, both at the ultrastructural and molecular level, two different cyanobacterial biofilms found in fissures of granite from continental Antarctica. Electron microscopy revealed structural differences between the two biofilms. One was only loosely adhered to the substrate, while the other biofilm showed a closer association between cells and rock minerals and was tightly attached to the substrate. Cells from both biofilms where ultrastructurally distinct, displaying, for instance, clear differences in their sheaths. The amounts of EPS and their organization associated with the cyanobacteria may determine the differences in adhesion and effects on the lithic substrate observed in the biofilms. By sequencing part of the 16S rRNA gene, the two cyanobacteria were also genetically characterized. The gene sequence of the cells comprising the biofilm that was tightly attached to the lithic substrate showed most homology with that of an endolithic cyanobacterium from Switzerland (AY153458), and the cyanobacterial type loosely adhered to the rock, clustered with Acaryochloris marina, the only organism unequivocally known to contain chlorophyll d. This study reveals the presence of at least two different types of endolithic biofilm, dominated each by a single type of cyanobacterium, able to withstand the harsh conditions of the Antarctic climate. Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Antarctica Digital.CSIC (Spanish National Research Council) Antarctic The Antarctic FEMS Microbiology Ecology 59 2 386 395 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Antarctica Cyanobacteria 16S rRNA Endolithic biofilms Extracellular polymeric substances |
spellingShingle |
Antarctica Cyanobacteria 16S rRNA Endolithic biofilms Extracellular polymeric substances Ríos, Asunción de los Grube, M. Sancho, Leopoldo G. Ascaso, Carmen Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
topic_facet |
Antarctica Cyanobacteria 16S rRNA Endolithic biofilms Extracellular polymeric substances |
description |
9 pages, and figures, and tables statistics The precise identification of the cyanobacteria that comprise an endolithic biofilm is hindered by difficulties in culturing the organisms found in these biofilms and a lack of previous molecular and ultrastructural data. This study characterizes, both at the ultrastructural and molecular level, two different cyanobacterial biofilms found in fissures of granite from continental Antarctica. Electron microscopy revealed structural differences between the two biofilms. One was only loosely adhered to the substrate, while the other biofilm showed a closer association between cells and rock minerals and was tightly attached to the substrate. Cells from both biofilms where ultrastructurally distinct, displaying, for instance, clear differences in their sheaths. The amounts of EPS and their organization associated with the cyanobacteria may determine the differences in adhesion and effects on the lithic substrate observed in the biofilms. By sequencing part of the 16S rRNA gene, the two cyanobacteria were also genetically characterized. The gene sequence of the cells comprising the biofilm that was tightly attached to the lithic substrate showed most homology with that of an endolithic cyanobacterium from Switzerland (AY153458), and the cyanobacterial type loosely adhered to the rock, clustered with Acaryochloris marina, the only organism unequivocally known to contain chlorophyll d. This study reveals the presence of at least two different types of endolithic biofilm, dominated each by a single type of cyanobacterium, able to withstand the harsh conditions of the Antarctic climate. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Ríos, Asunción de los Grube, M. Sancho, Leopoldo G. Ascaso, Carmen |
author_facet |
Ríos, Asunción de los Grube, M. Sancho, Leopoldo G. Ascaso, Carmen |
author_sort |
Ríos, Asunción de los |
title |
Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
title_short |
Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
title_full |
Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
title_fullStr |
Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
title_full_unstemmed |
Ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing Antarctic granite rocks |
title_sort |
ultrastructural and genetic characteristics of endolithic cyanobacterial biofilms colonizing antarctic granite rocks |
publisher |
Blackwell Publishing |
publishDate |
2007 |
url |
http://hdl.handle.net/10261/18369 https://doi.org/10.1111/j.1574-6941.2006.00256.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://dx.doi.org/10.1111/j.1574-6941.2006.00256.x FEMS Microbiology ecology 59: 386-395 (2007) 1574-6941 http://hdl.handle.net/10261/18369 doi:10.1111/j.1574-6941.2006.00256.x |
op_rights |
none |
op_doi |
https://doi.org/10.1111/j.1574-6941.2006.00256.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
59 |
container_issue |
2 |
container_start_page |
386 |
op_container_end_page |
395 |
_version_ |
1790593207314153472 |