Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability
The influence of low-frequency ultrasound (40 kHz) in the esterification reaction between acetic acid and butanol for flavor ester synthesis catalyzed by the commercial immobilized lipase B from Candida antarctica (Novozym 435) was evaluated. A central composite design and the response surface metho...
Published in: | Ultrasonics Sonochemistry |
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Online Access: | http://hdl.handle.net/10261/183447 https://doi.org/10.1016/j.ultsonch.2013.01.018 https://doi.org/10.13039/501100004263 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003593 |
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ftcsic:oai:digital.csic.es:10261/183447 2024-02-11T09:56:14+01:00 Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability Martins, Andrea B. Schein, Mirela F. Friedrich, John L. R. Fernández-Lafuente, Roberto Ayub, Marco A. Z. Rodrigues, Rafael C. Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Ministerio de Ciencia e Innovación (España) 2013-09 http://hdl.handle.net/10261/183447 https://doi.org/10.1016/j.ultsonch.2013.01.018 https://doi.org/10.13039/501100004263 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003593 unknown Elsevier https://doi.org/10.1016/j.ultsonch.2013.01.018 Ultrasonics Sonochemistry 20(5): 1155-1160 (2013) 1350-4177 http://hdl.handle.net/10261/183447 doi:10.1016/j.ultsonch.2013.01.018 1873-2828 http://dx.doi.org/10.13039/501100004263 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003593 none Enzyme stability Enzyme reuse Ultrasound Butyl acetate Esterification Lipase artículo http://purl.org/coar/resource_type/c_6501 2013 ftcsic https://doi.org/10.1016/j.ultsonch.2013.01.01810.13039/50110000426310.13039/50110000483710.13039/501100003593 2024-01-16T10:40:01Z The influence of low-frequency ultrasound (40 kHz) in the esterification reaction between acetic acid and butanol for flavor ester synthesis catalyzed by the commercial immobilized lipase B from Candida antarctica (Novozym 435) was evaluated. A central composite design and the response surface methodology were used to analyze the effects of the reaction parameters (temperature, substrate molar ratio, enzyme content and added water) and their response (yields of conversion in 2.5 h of reaction). The reaction was carried out using n-hexane as solvent. The optimal conditions for ultrasound-assisted butyl acetate synthesis were found to be: temperature of 46 °C; substrate molar ratio of 3.6:1 butanol:acetic acid; enzyme content of 7%; added water of 0.25%, conditions that are slightly different from those found using mechanical mixing. Over 94% of conversion was obtained in 2.5 h under these conditions. The optimal acid concentration for the reaction was determined to be 2.0 M, compared to 0.3 M without ultrasound treatment. Enzyme productivity was significantly improved to around 7.5-fold for each batch when comparing ultrasound and standard mechanical agitation. The biocatalyst could be directly reused for 14 reactions cycles keeping around 70% of its original activity, while activity was virtually zeroed in the third cycle using the standard mixing system. Thus, compared to the traditional mechanical agitation, ultrasound technology not only improves the process productivity, but also enhances enzyme recycling and stability in the presence of acetic acid, being a powerful tool to improve biocatalyst performance in this type of reaction. This work was supported by grants from Fundação de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS – ARD/2011), from CNPq (Brazilian Bureau of Science and Technology), and CTQ2009-07568 from Spanish Ministerio de Ciencia e Innovación. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Ultrasonics Sonochemistry 20 5 1155 1160 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Enzyme stability Enzyme reuse Ultrasound Butyl acetate Esterification Lipase |
spellingShingle |
Enzyme stability Enzyme reuse Ultrasound Butyl acetate Esterification Lipase Martins, Andrea B. Schein, Mirela F. Friedrich, John L. R. Fernández-Lafuente, Roberto Ayub, Marco A. Z. Rodrigues, Rafael C. Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
topic_facet |
Enzyme stability Enzyme reuse Ultrasound Butyl acetate Esterification Lipase |
description |
The influence of low-frequency ultrasound (40 kHz) in the esterification reaction between acetic acid and butanol for flavor ester synthesis catalyzed by the commercial immobilized lipase B from Candida antarctica (Novozym 435) was evaluated. A central composite design and the response surface methodology were used to analyze the effects of the reaction parameters (temperature, substrate molar ratio, enzyme content and added water) and their response (yields of conversion in 2.5 h of reaction). The reaction was carried out using n-hexane as solvent. The optimal conditions for ultrasound-assisted butyl acetate synthesis were found to be: temperature of 46 °C; substrate molar ratio of 3.6:1 butanol:acetic acid; enzyme content of 7%; added water of 0.25%, conditions that are slightly different from those found using mechanical mixing. Over 94% of conversion was obtained in 2.5 h under these conditions. The optimal acid concentration for the reaction was determined to be 2.0 M, compared to 0.3 M without ultrasound treatment. Enzyme productivity was significantly improved to around 7.5-fold for each batch when comparing ultrasound and standard mechanical agitation. The biocatalyst could be directly reused for 14 reactions cycles keeping around 70% of its original activity, while activity was virtually zeroed in the third cycle using the standard mixing system. Thus, compared to the traditional mechanical agitation, ultrasound technology not only improves the process productivity, but also enhances enzyme recycling and stability in the presence of acetic acid, being a powerful tool to improve biocatalyst performance in this type of reaction. This work was supported by grants from Fundação de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS – ARD/2011), from CNPq (Brazilian Bureau of Science and Technology), and CTQ2009-07568 from Spanish Ministerio de Ciencia e Innovación. Peer Reviewed |
author2 |
Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Ministerio de Ciencia e Innovación (España) |
format |
Article in Journal/Newspaper |
author |
Martins, Andrea B. Schein, Mirela F. Friedrich, John L. R. Fernández-Lafuente, Roberto Ayub, Marco A. Z. Rodrigues, Rafael C. |
author_facet |
Martins, Andrea B. Schein, Mirela F. Friedrich, John L. R. Fernández-Lafuente, Roberto Ayub, Marco A. Z. Rodrigues, Rafael C. |
author_sort |
Martins, Andrea B. |
title |
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
title_short |
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
title_full |
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
title_fullStr |
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
title_full_unstemmed |
Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability |
title_sort |
ultrasound-assisted butyl acetate synthesis catalyzed by novozym 435: enhanced activity and operational stability |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/183447 https://doi.org/10.1016/j.ultsonch.2013.01.018 https://doi.org/10.13039/501100004263 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003593 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://doi.org/10.1016/j.ultsonch.2013.01.018 Ultrasonics Sonochemistry 20(5): 1155-1160 (2013) 1350-4177 http://hdl.handle.net/10261/183447 doi:10.1016/j.ultsonch.2013.01.018 1873-2828 http://dx.doi.org/10.13039/501100004263 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003593 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.ultsonch.2013.01.01810.13039/50110000426310.13039/50110000483710.13039/501100003593 |
container_title |
Ultrasonics Sonochemistry |
container_volume |
20 |
container_issue |
5 |
container_start_page |
1155 |
op_container_end_page |
1160 |
_version_ |
1790601575291420672 |