Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain
There is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites Giardia duodenalis and Cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. Consequently, the extent of the cy...
Published in: | Veterinary Parasitology |
---|---|
Main Authors: | , , , , , , , , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier
2017
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/174361 https://doi.org/10.1016/j.vetpar.2017.01.016 https://doi.org/10.13039/100007652 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100008049 https://doi.org/10.13039/501100004587 |
id |
ftcsic:oai:digital.csic.es:10261/174361 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/174361 2024-02-11T10:02:49+01:00 Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain Mateo, Marta Hernández de Mingo, Marta Lucio, Aida de Morales, Lucía Balseiro, Ana Espí, Alberto Barral, Marta Lima-Barbero, José Francisco Habela, Miguel Ángel Fernández-García, José L. Calero Bernal, Rafael Köster, Pamela C. Cardona, Guillermo A. Carmena, David Instituto de Salud Carlos III Universidad Alfonso X El Sabio Fundación Banco Santander CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Economía y Competitividad (España) European Commission 2017 http://hdl.handle.net/10261/174361 https://doi.org/10.1016/j.vetpar.2017.01.016 https://doi.org/10.13039/100007652 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100008049 https://doi.org/10.13039/501100004587 unknown Elsevier Sí doi:10.1016/j.vetpar.2017.01.016 issn: 0304-4017 Veterinary Parasitology 235: 86-93 (2017) http://hdl.handle.net/10261/174361 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100008049 http://dx.doi.org/10.13039/501100004587 28215875 none Cryptosporidium Epidemiology Giardia duodenalis Mesocarnivores Wildlife Spain Zoonotic transmission Genotyping artículo http://purl.org/coar/resource_type/c_6501 2017 ftcsic https://doi.org/10.1016/j.vetpar.2017.01.01610.13039/10000765210.13039/50110000078010.13039/50110000332910.13039/10000804910.13039/501100004587 2024-01-16T10:35:11Z There is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites Giardia duodenalis and Cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. Consequently, the extent of the cyst/oocyst environmental contamination attributable to these wild host species and their potential implications for public veterinary health remain largely unknown. In this molecular epidemiological survey a total of 193 individual faecal samples from badgers (Meles meles, n = 70), ferrets (Mustela putorius furo, n = 2), genets (Genetta genetta, n = 6), Iberian lynxes (Lynx pardinus, n = 6), beech martens (Martes foina, n = 8), mongooses (Herpestes ichneumon, n = 2), otters (Lutra lutra, n = 2), polecats (Mustela putorius, n = 2), red foxes (Vulpes vulpes, n = 87), wildcats (Felis silvestris, n = 2), and wolves (Canis lupus, n = 6) were obtained from road-killed, hunted, and accidentally found carcasses, and from camera-trap surveys or animals entering rescue shelters, during the period December 2003–April 2016. Investigated specimens were collected in five Spanish autonomous regions including Andalusia (n = 1), Asturias (n = 69), Basque Country (n = 49), Castile-La Mancha (n = 38), and Extremadura (n = 36). The presence of cysts/oocysts was confirmed by PCR-based methods targeting the small subunit (ssu) ribosomal RNA gene of these parasite species. Genotyping of the obtained isolates were attempted at appropriate markers including the glutamate dehydrogenase (G. duodenalis) and the 60-kDa glycoprotein (C. parvum and C. ubiquitum) loci. Overall, G. duodenalis was detected in 8% (7/87) of red foxes, a single beech marten, and a single wolf, respectively. Cryptosporidium was identified in 3% (2/70) of badgers, 8% (7/87) of red foxes, a single genet, and a single mongoose, respectively. None of the nine G. duodenalis isolates generated could be genotyped at the assemblage/sub-assemblage level. Out of the nine ... Article in Journal/Newspaper Canis lupus Lutra lutra Lynx Digital.CSIC (Spanish National Research Council) Veterinary Parasitology 235 86 93 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Cryptosporidium Epidemiology Giardia duodenalis Mesocarnivores Wildlife Spain Zoonotic transmission Genotyping |
spellingShingle |
Cryptosporidium Epidemiology Giardia duodenalis Mesocarnivores Wildlife Spain Zoonotic transmission Genotyping Mateo, Marta Hernández de Mingo, Marta Lucio, Aida de Morales, Lucía Balseiro, Ana Espí, Alberto Barral, Marta Lima-Barbero, José Francisco Habela, Miguel Ángel Fernández-García, José L. Calero Bernal, Rafael Köster, Pamela C. Cardona, Guillermo A. Carmena, David Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
topic_facet |
Cryptosporidium Epidemiology Giardia duodenalis Mesocarnivores Wildlife Spain Zoonotic transmission Genotyping |
description |
There is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites Giardia duodenalis and Cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. Consequently, the extent of the cyst/oocyst environmental contamination attributable to these wild host species and their potential implications for public veterinary health remain largely unknown. In this molecular epidemiological survey a total of 193 individual faecal samples from badgers (Meles meles, n = 70), ferrets (Mustela putorius furo, n = 2), genets (Genetta genetta, n = 6), Iberian lynxes (Lynx pardinus, n = 6), beech martens (Martes foina, n = 8), mongooses (Herpestes ichneumon, n = 2), otters (Lutra lutra, n = 2), polecats (Mustela putorius, n = 2), red foxes (Vulpes vulpes, n = 87), wildcats (Felis silvestris, n = 2), and wolves (Canis lupus, n = 6) were obtained from road-killed, hunted, and accidentally found carcasses, and from camera-trap surveys or animals entering rescue shelters, during the period December 2003–April 2016. Investigated specimens were collected in five Spanish autonomous regions including Andalusia (n = 1), Asturias (n = 69), Basque Country (n = 49), Castile-La Mancha (n = 38), and Extremadura (n = 36). The presence of cysts/oocysts was confirmed by PCR-based methods targeting the small subunit (ssu) ribosomal RNA gene of these parasite species. Genotyping of the obtained isolates were attempted at appropriate markers including the glutamate dehydrogenase (G. duodenalis) and the 60-kDa glycoprotein (C. parvum and C. ubiquitum) loci. Overall, G. duodenalis was detected in 8% (7/87) of red foxes, a single beech marten, and a single wolf, respectively. Cryptosporidium was identified in 3% (2/70) of badgers, 8% (7/87) of red foxes, a single genet, and a single mongoose, respectively. None of the nine G. duodenalis isolates generated could be genotyped at the assemblage/sub-assemblage level. Out of the nine ... |
author2 |
Instituto de Salud Carlos III Universidad Alfonso X El Sabio Fundación Banco Santander CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Economía y Competitividad (España) European Commission |
format |
Article in Journal/Newspaper |
author |
Mateo, Marta Hernández de Mingo, Marta Lucio, Aida de Morales, Lucía Balseiro, Ana Espí, Alberto Barral, Marta Lima-Barbero, José Francisco Habela, Miguel Ángel Fernández-García, José L. Calero Bernal, Rafael Köster, Pamela C. Cardona, Guillermo A. Carmena, David |
author_facet |
Mateo, Marta Hernández de Mingo, Marta Lucio, Aida de Morales, Lucía Balseiro, Ana Espí, Alberto Barral, Marta Lima-Barbero, José Francisco Habela, Miguel Ángel Fernández-García, José L. Calero Bernal, Rafael Köster, Pamela C. Cardona, Guillermo A. Carmena, David |
author_sort |
Mateo, Marta |
title |
Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
title_short |
Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
title_full |
Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
title_fullStr |
Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
title_full_unstemmed |
Occurrence and molecular genotyping of Giardia duodenalis and Cryptosporidium spp. in wild mesocarnivores in Spain |
title_sort |
occurrence and molecular genotyping of giardia duodenalis and cryptosporidium spp. in wild mesocarnivores in spain |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/174361 https://doi.org/10.1016/j.vetpar.2017.01.016 https://doi.org/10.13039/100007652 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100008049 https://doi.org/10.13039/501100004587 |
genre |
Canis lupus Lutra lutra Lynx |
genre_facet |
Canis lupus Lutra lutra Lynx |
op_relation |
Sí doi:10.1016/j.vetpar.2017.01.016 issn: 0304-4017 Veterinary Parasitology 235: 86-93 (2017) http://hdl.handle.net/10261/174361 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100008049 http://dx.doi.org/10.13039/501100004587 28215875 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.vetpar.2017.01.01610.13039/10000765210.13039/50110000078010.13039/50110000332910.13039/10000804910.13039/501100004587 |
container_title |
Veterinary Parasitology |
container_volume |
235 |
container_start_page |
86 |
op_container_end_page |
93 |
_version_ |
1790598882463318016 |