Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries
Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, w...
Published in: | Veterinary Microbiology |
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Online Access: | http://hdl.handle.net/10261/174101 https://doi.org/10.1016/j.vetmic.2017.07.001 https://doi.org/10.13039/100009139 |
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ftcsic:oai:digital.csic.es:10261/174101 2024-02-11T10:08:13+01:00 Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz Fons, Francisco Pikula, Jiri Knap, Nataša Tsakmakidis, Ioannis. Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. German Center for Infection Research 2017 http://hdl.handle.net/10261/174101 https://doi.org/10.1016/j.vetmic.2017.07.001 https://doi.org/10.13039/100009139 unknown Elsevier Sí doi:10.1016/j.vetmic.2017.07.001 e-issn: 1873-2542 issn: 0378-1135 Veterinary Microbiology 208: 58-68 (2017) http://hdl.handle.net/10261/174101 http://dx.doi.org/10.13039/100009139 28888650 none Europe Norway rat Black rat Genotype 3 Hepatitis E virus Hepeviridae artículo http://purl.org/coar/resource_type/c_6501 2017 ftcsic https://doi.org/10.1016/j.vetmic.2017.07.00110.13039/100009139 2024-01-16T10:34:59Z Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broad-spectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated. The investigations in the laboratory of Rainer G. Ulrich were supported by the German Center for Infection Research (DZIF). Peer Reviewed Article in Journal/Newspaper Rattus rattus Digital.CSIC (Spanish National Research Council) Norway Veterinary Microbiology 208 58 68 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Europe Norway rat Black rat Genotype 3 Hepatitis E virus Hepeviridae |
spellingShingle |
Europe Norway rat Black rat Genotype 3 Hepatitis E virus Hepeviridae Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz Fons, Francisco Pikula, Jiri Knap, Nataša Tsakmakidis, Ioannis. Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
topic_facet |
Europe Norway rat Black rat Genotype 3 Hepatitis E virus Hepeviridae |
description |
Rat hepatitis E virus (HEV) is genetically only distantly related to hepeviruses found in other mammalian reservoirs and in humans. It was initially detected in Norway rats (Rattus norvegicus) from Germany, and subsequently in rats from Vietnam, the USA, Indonesia, China, Denmark and France. Here, we report on a molecular survey of Norway rats and Black rats (Rattus rattus) from 12 European countries for ratHEV and human pathogenic hepeviruses. RatHEV-specific real-time and conventional RT-PCR investigations revealed the presence of ratHEV in 63 of 508 (12.4%) rats at the majority of sites in 11 of 12 countries. In contrast, a real-time RT-PCR specific for human pathogenic HEV genotypes 1–4 and a nested broad-spectrum (NBS) RT-PCR with subsequent sequence determination did not detect any infections with these genotypes. Only in a single Norway rat from Belgium a rabbit HEV-like genotype 3 sequence was detected. Phylogenetic analysis indicated a clustering of all other novel Norway and Black rat-derived sequences with ratHEV sequences from Europe, the USA and a Black rat-derived sequence from Indonesia within the proposed ratHEV genotype 1. No difference in infection status was detected related to age, sex, rat species or density of human settlements and zoological gardens. In conclusion, our investigation shows a broad geographical distribution of ratHEV in Norway and Black rats from Europe and its presence in all settlement types investigated. The investigations in the laboratory of Rainer G. Ulrich were supported by the German Center for Infection Research (DZIF). Peer Reviewed |
author2 |
German Center for Infection Research |
format |
Article in Journal/Newspaper |
author |
Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz Fons, Francisco Pikula, Jiri Knap, Nataša Tsakmakidis, Ioannis. Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. |
author_facet |
Ryll, René Bernstein, Samuel Heuser, Elisa Schlegel, Mathias Dremsek, Paul Zumpe, Maxi Wolf, Sandro Pépin, Michel Bajomi, Daniel Müller, Gabi Heiberg, Ann-Charlotte Spahr, Carina Lang, Johannes Groschup, Martin H. Ansorge, Hermann Freise, Jona Guenther, Sebastian Baert, Kristof Ruiz Fons, Francisco Pikula, Jiri Knap, Nataša Tsakmakidis, Ioannis. Dovas, Chrysostomos Zanet, Stefania Imholt, Christian Heckel, Gerald Johne, Reimar Ulrich, Rainer G. |
author_sort |
Ryll, René |
title |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_short |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_full |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_fullStr |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_full_unstemmed |
Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries |
title_sort |
detection of rat hepatitis e virus in wild norway rats (rattus norvegicus) and black rats (rattus rattus) from 11 european countries |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/174101 https://doi.org/10.1016/j.vetmic.2017.07.001 https://doi.org/10.13039/100009139 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
Sí doi:10.1016/j.vetmic.2017.07.001 e-issn: 1873-2542 issn: 0378-1135 Veterinary Microbiology 208: 58-68 (2017) http://hdl.handle.net/10261/174101 http://dx.doi.org/10.13039/100009139 28888650 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.vetmic.2017.07.00110.13039/100009139 |
container_title |
Veterinary Microbiology |
container_volume |
208 |
container_start_page |
58 |
op_container_end_page |
68 |
_version_ |
1790607229787832320 |