Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm
The development of a species-specific conservation protocol that involves artificial insemination with frozen semen needs to validate an effective methodology for freezing semen. Colloid centrifugation has been suggested and widely applied as an effective tool for selecting animal spermatozoa for ar...
Published in: | Theriogenology |
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Elsevier
2016
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Online Access: | http://hdl.handle.net/10261/173957 https://doi.org/10.1016/j.theriogenology.2015.11.021 https://doi.org/10.13039/501100003329 |
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ftcsic:oai:digital.csic.es:10261/173957 2024-02-11T10:09:20+01:00 Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm Álvarez-Rodríguez, Manuel Álvarez, Mercedes Anel-López, Luis López-Urueña, Elena Manrique, P. Borragán, Santiago Morrell, J. M. Paz, Paulino de Anel, Luis Ministerio de Economía y Competitividad (España) Sociedad Regional Cántabra de Promoción Turística 2016 http://hdl.handle.net/10261/173957 https://doi.org/10.1016/j.theriogenology.2015.11.021 https://doi.org/10.13039/501100003329 unknown Elsevier #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2013-48255-R Sí doi:10.1016/j.theriogenology.2015.11.021 issn: 0093-691X e-issn: 1879-3231 Theriogenology 85(6): 1097-1105 (2016) http://hdl.handle.net/10261/173957 http://dx.doi.org/10.13039/501100003329 26764151 none Percoll Freezability PureSperm Androcoll Brown bear sperm artículo http://purl.org/coar/resource_type/c_6501 2016 ftcsic https://doi.org/10.1016/j.theriogenology.2015.11.02110.13039/501100003329 2024-01-16T10:34:56Z The development of a species-specific conservation protocol that involves artificial insemination with frozen semen needs to validate an effective methodology for freezing semen. Colloid centrifugation has been suggested and widely applied as an effective tool for selecting animal spermatozoa for artificial breeding. The objective of the present study was to compare different methods of centrifugation, single layer using Androcoll-Bear and Percoll and double layer using PureSperm 100 (in two different discontinuous gradients 40%-80% and 45%-90%), for the selection of fresh brown bear sperm samples. In the before freezing group, all selected samples showed a higher progressive motility and viability (except Percoll for motility 43.0 ± 5.3 [P < 0.05]); all colloids except PureSperm 45/90% rendered samples with fewer damaged acrosomes. In the after thawing group, all tested centrifugation colloids showed a good capacity to decrease the number of damaged acrosomes. Furthermore, PureSperm treatment (45/90%) resulted in an increase in apoptotic-like changes not only immediately after thawing but also after the incubation test, leading us to suggest that this gradient could induce some kind of deleterious effects on the sperm samples. On the other hand, PureSperm treatment (40/80%) yielded a quality preservation capacity similar to Androcoll-Bear in number of damaged acrosomes, different relative to the control (control, 5.3 ± 0.6; PureSperm 80, 2.0 ± 0.3; Androcoll, 2.1 ± 0.9 [P < 0.05]) but a decrease in the number of viable spermatozoa recovered after thawing relative to the control (control, 21.2 ± 3.1; PureSperm 80, 13.7 ± 2.7 [P < 0.05]). In conclusion, Androcoll-Bear constitutes a useful tool for handling of brown bear ejaculates owing to its simple handling and procedure with a reliable sperm selection and freezability. This colloid yielded an improvement in several sperm parameters in brown bear frozen-thawed semen; the selected spermatozoa of fresh samples with this colloid showed a better resistance ... Article in Journal/Newspaper Ursus arctos Digital.CSIC (Spanish National Research Council) Theriogenology 85 6 1097 1105 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Percoll Freezability PureSperm Androcoll Brown bear sperm |
spellingShingle |
Percoll Freezability PureSperm Androcoll Brown bear sperm Álvarez-Rodríguez, Manuel Álvarez, Mercedes Anel-López, Luis López-Urueña, Elena Manrique, P. Borragán, Santiago Morrell, J. M. Paz, Paulino de Anel, Luis Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
topic_facet |
Percoll Freezability PureSperm Androcoll Brown bear sperm |
description |
The development of a species-specific conservation protocol that involves artificial insemination with frozen semen needs to validate an effective methodology for freezing semen. Colloid centrifugation has been suggested and widely applied as an effective tool for selecting animal spermatozoa for artificial breeding. The objective of the present study was to compare different methods of centrifugation, single layer using Androcoll-Bear and Percoll and double layer using PureSperm 100 (in two different discontinuous gradients 40%-80% and 45%-90%), for the selection of fresh brown bear sperm samples. In the before freezing group, all selected samples showed a higher progressive motility and viability (except Percoll for motility 43.0 ± 5.3 [P < 0.05]); all colloids except PureSperm 45/90% rendered samples with fewer damaged acrosomes. In the after thawing group, all tested centrifugation colloids showed a good capacity to decrease the number of damaged acrosomes. Furthermore, PureSperm treatment (45/90%) resulted in an increase in apoptotic-like changes not only immediately after thawing but also after the incubation test, leading us to suggest that this gradient could induce some kind of deleterious effects on the sperm samples. On the other hand, PureSperm treatment (40/80%) yielded a quality preservation capacity similar to Androcoll-Bear in number of damaged acrosomes, different relative to the control (control, 5.3 ± 0.6; PureSperm 80, 2.0 ± 0.3; Androcoll, 2.1 ± 0.9 [P < 0.05]) but a decrease in the number of viable spermatozoa recovered after thawing relative to the control (control, 21.2 ± 3.1; PureSperm 80, 13.7 ± 2.7 [P < 0.05]). In conclusion, Androcoll-Bear constitutes a useful tool for handling of brown bear ejaculates owing to its simple handling and procedure with a reliable sperm selection and freezability. This colloid yielded an improvement in several sperm parameters in brown bear frozen-thawed semen; the selected spermatozoa of fresh samples with this colloid showed a better resistance ... |
author2 |
Ministerio de Economía y Competitividad (España) Sociedad Regional Cántabra de Promoción Turística |
format |
Article in Journal/Newspaper |
author |
Álvarez-Rodríguez, Manuel Álvarez, Mercedes Anel-López, Luis López-Urueña, Elena Manrique, P. Borragán, Santiago Morrell, J. M. Paz, Paulino de Anel, Luis |
author_facet |
Álvarez-Rodríguez, Manuel Álvarez, Mercedes Anel-López, Luis López-Urueña, Elena Manrique, P. Borragán, Santiago Morrell, J. M. Paz, Paulino de Anel, Luis |
author_sort |
Álvarez-Rodríguez, Manuel |
title |
Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
title_short |
Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
title_full |
Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
title_fullStr |
Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
title_full_unstemmed |
Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm |
title_sort |
effect of colloid (androcoll-bear, percoll, and puresperm) selection on the freezability of brown bear (ursus arctos) sperm |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/173957 https://doi.org/10.1016/j.theriogenology.2015.11.021 https://doi.org/10.13039/501100003329 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2013-48255-R Sí doi:10.1016/j.theriogenology.2015.11.021 issn: 0093-691X e-issn: 1879-3231 Theriogenology 85(6): 1097-1105 (2016) http://hdl.handle.net/10261/173957 http://dx.doi.org/10.13039/501100003329 26764151 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.theriogenology.2015.11.02110.13039/501100003329 |
container_title |
Theriogenology |
container_volume |
85 |
container_issue |
6 |
container_start_page |
1097 |
op_container_end_page |
1105 |
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1790609198175748096 |