Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering
The immobilization of biocatalysts on magnetic nanomaterial surface is a very attractive alternative to achieve enzyme nanoderivatives with highly improved properties. The combination between the careful tailoring of nanocarrier surfaces and the site-specific chemical modification of biomacromolecul...
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Multidisciplinary Digital Publishing Institute
2018
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Online Access: | http://hdl.handle.net/10261/166925 https://doi.org/10.3390/polym10060615 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100002911 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/100012818 |
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ftcsic:oai:digital.csic.es:10261/166925 2024-02-11T09:58:29+01:00 Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering Viñambres, Mario Filice, Marco Marciello, Marzia Ministerio de Economía y Competitividad (España) European Commission Comunidad de Madrid Universidad Complutense de Madrid Consejo Superior de Investigaciones Científicas (España) Fundación Severo Ochoa Fundación Pro CNIC 2018-06-05 http://hdl.handle.net/10261/166925 https://doi.org/10.3390/polym10060615 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100002911 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/100012818 unknown Multidisciplinary Digital Publishing Institute #PLACEHOLDER_PARENT_METADATA_VALUE# 2017-T1/BIO-4992 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-59118-JIN Publisher's version http://dx.doi.org/10.3390/polym10060615 doi:10.3390/polym10060615 Polymers 10 (6): 615 (2018) http://hdl.handle.net/10261/166925 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100002911 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/100012818 30966649 open artículo http://purl.org/coar/resource_type/c_6501 2018 ftcsic https://doi.org/10.3390/polym1006061510.13039/50110000332910.13039/50110000078010.13039/50110000291110.13039/50110000333910.13039/100012818 2024-01-16T10:31:28Z The immobilization of biocatalysts on magnetic nanomaterial surface is a very attractive alternative to achieve enzyme nanoderivatives with highly improved properties. The combination between the careful tailoring of nanocarrier surfaces and the site-specific chemical modification of biomacromolecules is a crucial parameter to finely modulate the catalytic behavior of the biocatalyst. In this work, a useful strategy to immobilize chemically aminated lipase B from Candida antarctica on magnetic iron oxide nanoparticles (IONPs) by covalent multipoint attachment or hydrophobic physical adsorption upon previous tailored engineering of nanocarriers with poly-carboxylic groups (citric acid or succinic anhydride, CALB EDA @CA-NPs and CALB EDA @SA-NPs respectively) or hydrophobic layer (oleic acid, CALB EDA @OA-NPs) is described. After full characterization, the nanocatalysts have been assessed in the enantioselective kinetic resolution of racemic methyl mandelate. Depending on the immobilization strategy, each enzymatic nanoderivative permitted to selectively improve a specific property of the biocatalyst. In general, all the immobilization protocols permitted loading from good to high lipase amount (149 < immobilized lipase < 234 mg/g Fe ). The hydrophobic CALB EDA @OA-NPs was the most active nanocatalyst, whereas the covalent CALB EDA @CA-NPs and CALB EDA @SA-NPs were revealed to be the most thermostable and also the most enantioselective ones in the kinetic resolution reaction (almost 90% ee R-enantiomer). A strategy to maintain all these properties in long-time storage (up to 1 month) by freeze-drying was also optimized. Therefore, the nanocarrier surface engineering is demonstrated to be a key-parameter in the design and preparation of lipase libraries with enhanced catalytic properties. The CNIC is supported by Spanish Ministry for Economy and Competitiveness (MEyC) and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). M.F. would like to thank MEyC for the research grant ... Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Polymers 10 6 615 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
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description |
The immobilization of biocatalysts on magnetic nanomaterial surface is a very attractive alternative to achieve enzyme nanoderivatives with highly improved properties. The combination between the careful tailoring of nanocarrier surfaces and the site-specific chemical modification of biomacromolecules is a crucial parameter to finely modulate the catalytic behavior of the biocatalyst. In this work, a useful strategy to immobilize chemically aminated lipase B from Candida antarctica on magnetic iron oxide nanoparticles (IONPs) by covalent multipoint attachment or hydrophobic physical adsorption upon previous tailored engineering of nanocarriers with poly-carboxylic groups (citric acid or succinic anhydride, CALB EDA @CA-NPs and CALB EDA @SA-NPs respectively) or hydrophobic layer (oleic acid, CALB EDA @OA-NPs) is described. After full characterization, the nanocatalysts have been assessed in the enantioselective kinetic resolution of racemic methyl mandelate. Depending on the immobilization strategy, each enzymatic nanoderivative permitted to selectively improve a specific property of the biocatalyst. In general, all the immobilization protocols permitted loading from good to high lipase amount (149 < immobilized lipase < 234 mg/g Fe ). The hydrophobic CALB EDA @OA-NPs was the most active nanocatalyst, whereas the covalent CALB EDA @CA-NPs and CALB EDA @SA-NPs were revealed to be the most thermostable and also the most enantioselective ones in the kinetic resolution reaction (almost 90% ee R-enantiomer). A strategy to maintain all these properties in long-time storage (up to 1 month) by freeze-drying was also optimized. Therefore, the nanocarrier surface engineering is demonstrated to be a key-parameter in the design and preparation of lipase libraries with enhanced catalytic properties. The CNIC is supported by Spanish Ministry for Economy and Competitiveness (MEyC) and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). M.F. would like to thank MEyC for the research grant ... |
author2 |
Ministerio de Economía y Competitividad (España) European Commission Comunidad de Madrid Universidad Complutense de Madrid Consejo Superior de Investigaciones Científicas (España) Fundación Severo Ochoa Fundación Pro CNIC |
format |
Article in Journal/Newspaper |
author |
Viñambres, Mario Filice, Marco Marciello, Marzia |
spellingShingle |
Viñambres, Mario Filice, Marco Marciello, Marzia Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
author_facet |
Viñambres, Mario Filice, Marco Marciello, Marzia |
author_sort |
Viñambres, Mario |
title |
Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
title_short |
Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
title_full |
Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
title_fullStr |
Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
title_full_unstemmed |
Modulation of the Catalytic Properties of Lipase B from Candida antarctica by Immobilization on Tailor-Made Magnetic Iron Oxide Nanoparticles: The Key Role of Nanocarrier Surface Engineering |
title_sort |
modulation of the catalytic properties of lipase b from candida antarctica by immobilization on tailor-made magnetic iron oxide nanoparticles: the key role of nanocarrier surface engineering |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
http://hdl.handle.net/10261/166925 https://doi.org/10.3390/polym10060615 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100002911 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/100012818 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# 2017-T1/BIO-4992 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-59118-JIN Publisher's version http://dx.doi.org/10.3390/polym10060615 doi:10.3390/polym10060615 Polymers 10 (6): 615 (2018) http://hdl.handle.net/10261/166925 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100002911 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/100012818 30966649 |
op_rights |
open |
op_doi |
https://doi.org/10.3390/polym1006061510.13039/50110000332910.13039/50110000078010.13039/50110000291110.13039/50110000333910.13039/100012818 |
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Polymers |
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10 |
container_issue |
6 |
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615 |
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1790594146429304832 |