Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean

7 pages, 5 figures, 1 table.-- Issue title: "Sea ice and life in a river-influenced arctic shelf ecosystem" The ability of bacteria to assimilate sulfur from dimethylsulfoniopropionate (DMSP) was examined in the western Arctic Ocean by combining microautoradiography and fluorescence in sit...

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Published in:Journal of Marine Systems
Main Authors: Vila-Costa, Maria, Simó, Rafel, Alonso-Sáez, Laura, Pedrós-Alió, Carlos
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2008
Subjects:
Online Access:http://hdl.handle.net/10261/16131
https://doi.org/10.1016/j.jmarsys.2007.10.006
id ftcsic:oai:digital.csic.es:10261/16131
record_format openpolar
spelling ftcsic:oai:digital.csic.es:10261/16131 2024-02-11T10:00:18+01:00 Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean Vila-Costa, Maria Simó, Rafel Alonso-Sáez, Laura Pedrós-Alió, Carlos 2008-12-01 22195 bytes application/pdf http://hdl.handle.net/10261/16131 https://doi.org/10.1016/j.jmarsys.2007.10.006 en eng Elsevier https://doi.org/10.1016/j.jmarsys.2007.10.006 Journal of Marine Systems 74(3-4): 957-963 (2008) http://hdl.handle.net/10261/16131 0924-7963 doi:10.1016/j.jmarsys.2007.10.006 none Bacteria DMSP Leucine Microautoradiography FISH Beaufort Sea Franklin Bay Arctic winter artículo http://purl.org/coar/resource_type/c_6501 2008 ftcsic https://doi.org/10.1016/j.jmarsys.2007.10.006 2024-01-16T09:23:20Z 7 pages, 5 figures, 1 table.-- Issue title: "Sea ice and life in a river-influenced arctic shelf ecosystem" The ability of bacteria to assimilate sulfur from dimethylsulfoniopropionate (DMSP) was examined in the western Arctic Ocean by combining microautoradiography and fluorescence in situ hybridization (FISH). Assimilation of leucine was also measured for comparative purposes since leucine is considered a universal substrate for bacteria, which use it for protein synthesis. Samples were collected at 3 m depth, through a hole in the ice, in the CASES (Canadian Arctic Shelf Ecosystem Study) overwintering station in Franklin Bay (eastern Beaufort Sea) in March and May 2004 to compare two contrasting situations: winter and early spring. FISH counts indicated that the bacterial assemblage consisted of α- (up to 60% of the EUB positive cells), β- (up to 10%) and γ-proteobacteria (around 20%), and Bacteroidetes (up to 60%). The β-proteobacteria were not active with any of the two substrates tested. The remaining groups were much less efficient at assimilating DMSP-sulfur (5% of the cells) than leucine (20–35%) both in winter and in spring. Only the Roseobacter group of α-proteobacteria showed a similar assimilation of both substrates Financial support for this study was provided by grants from the Generalitat de Catalunya (DURSI 2003ACES00029/ANT), the Spanish Ministerio de Educación y Ciencia (REN2002-11565-E/ANT), and the Natural Sciences and Engineering Research Council of Canada (NSERC) through project CASES (Canadian Arctic Shelf Exchange Study) under the overall direction of L.Fortier. Special thanks to W. F. Vincent for providing the opportunity to join CASES Peer reviewed Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea Franklin Bay Sea ice Digital.CSIC (Spanish National Research Council) Arctic Arctic Ocean Canada Journal of Marine Systems 74 3-4 957 963
institution Open Polar
collection Digital.CSIC (Spanish National Research Council)
op_collection_id ftcsic
language English
topic Bacteria
DMSP
Leucine
Microautoradiography
FISH
Beaufort Sea
Franklin Bay
Arctic winter
spellingShingle Bacteria
DMSP
Leucine
Microautoradiography
FISH
Beaufort Sea
Franklin Bay
Arctic winter
Vila-Costa, Maria
Simó, Rafel
Alonso-Sáez, Laura
Pedrós-Alió, Carlos
Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
topic_facet Bacteria
DMSP
Leucine
Microautoradiography
FISH
Beaufort Sea
Franklin Bay
Arctic winter
description 7 pages, 5 figures, 1 table.-- Issue title: "Sea ice and life in a river-influenced arctic shelf ecosystem" The ability of bacteria to assimilate sulfur from dimethylsulfoniopropionate (DMSP) was examined in the western Arctic Ocean by combining microautoradiography and fluorescence in situ hybridization (FISH). Assimilation of leucine was also measured for comparative purposes since leucine is considered a universal substrate for bacteria, which use it for protein synthesis. Samples were collected at 3 m depth, through a hole in the ice, in the CASES (Canadian Arctic Shelf Ecosystem Study) overwintering station in Franklin Bay (eastern Beaufort Sea) in March and May 2004 to compare two contrasting situations: winter and early spring. FISH counts indicated that the bacterial assemblage consisted of α- (up to 60% of the EUB positive cells), β- (up to 10%) and γ-proteobacteria (around 20%), and Bacteroidetes (up to 60%). The β-proteobacteria were not active with any of the two substrates tested. The remaining groups were much less efficient at assimilating DMSP-sulfur (5% of the cells) than leucine (20–35%) both in winter and in spring. Only the Roseobacter group of α-proteobacteria showed a similar assimilation of both substrates Financial support for this study was provided by grants from the Generalitat de Catalunya (DURSI 2003ACES00029/ANT), the Spanish Ministerio de Educación y Ciencia (REN2002-11565-E/ANT), and the Natural Sciences and Engineering Research Council of Canada (NSERC) through project CASES (Canadian Arctic Shelf Exchange Study) under the overall direction of L.Fortier. Special thanks to W. F. Vincent for providing the opportunity to join CASES Peer reviewed
format Article in Journal/Newspaper
author Vila-Costa, Maria
Simó, Rafel
Alonso-Sáez, Laura
Pedrós-Alió, Carlos
author_facet Vila-Costa, Maria
Simó, Rafel
Alonso-Sáez, Laura
Pedrós-Alió, Carlos
author_sort Vila-Costa, Maria
title Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
title_short Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
title_full Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
title_fullStr Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
title_full_unstemmed Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean
title_sort number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal arctic ocean
publisher Elsevier
publishDate 2008
url http://hdl.handle.net/10261/16131
https://doi.org/10.1016/j.jmarsys.2007.10.006
geographic Arctic
Arctic Ocean
Canada
geographic_facet Arctic
Arctic Ocean
Canada
genre Arctic
Arctic Ocean
Beaufort Sea
Franklin Bay
Sea ice
genre_facet Arctic
Arctic Ocean
Beaufort Sea
Franklin Bay
Sea ice
op_relation https://doi.org/10.1016/j.jmarsys.2007.10.006
Journal of Marine Systems 74(3-4): 957-963 (2008)
http://hdl.handle.net/10261/16131
0924-7963
doi:10.1016/j.jmarsys.2007.10.006
op_rights none
op_doi https://doi.org/10.1016/j.jmarsys.2007.10.006
container_title Journal of Marine Systems
container_volume 74
container_issue 3-4
container_start_page 957
op_container_end_page 963
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