Advances in the knowledge of the antiviral immune response and resistance to Viral Haemorrhagic Septicaemia Virus (VHSV) in turbot (Scophthalmus maximus)

• Main Author: Pereiro, Patricia
• Other Authors: Novoa, Beatriz, Figueras, Antonio, Ministerio de Economía y Competitividad (España)
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: Universidad de Santiago de Compostela 2017
• Subjects: Turbot; VHSV; Immune response; Type I interferons; Interferon-gamma; Scophthalmus maximus
• Online Access: http://hdl.handle.net/10261/153756; https://doi.org/10.13039/501100003329

185 páginas [EN] Turbot (Scophthalmus maximus) is an economically valuable fish in Europe and China. Currently, the culture of this fish is well established, although several pathogens can affect its health status, causing important economic losses in the sector. Viral Haemorrhagic Septicaemia Virus (VHSV) is one of the main threats in turbot farms due to the absence of commercially available treatments and vaccines for VHSV. The first goal of this doctoral thesis was to increase the amount of information available in public databases regarding the transcriptome sequences associated with the antiviral immune response of turbot. Due to the large number of sequences obtained, a microarray highly enriched in antiviral sequences was constructed. This microarray allowed us to conduct a broad transcriptome analysis of the response to VHSV infection and evaluate the activity of a DNA vaccine against VHSV, which was also designed during this doctoral thesis. This information led us to focus our attention on certain molecules or processes affected by the vaccine/infection. This was the case for two type I interferons (IFNs), which were characterized and studied for the first time in turbot. Type I IFNs are the main antiviral molecules in vertebrates, as they induce the expression of numerous molecules with the ability to block viral proliferation. To increase our knowledge about these genes, we also sought to investigate the role of the type II IFN (or IFN-gamma), which also acts in the defence against viruses but mainly functions as an immunomodulatory molecule [ES] El rodaballo (Scophthalmus maximus) es un pez con un alto valor comercial principalmente en Europa y China. Aunque actualmente su cultivo está bien establecido, diversos patógenos pueden afectar a su estado sanitario, ocasionando importantes pérdidas económicas en el sector. El virus de la septicemia hemorrágica viral (VHSV) es una de las principales amenazas en su cultivo, ya que no existen tratamientos ni vacunas comerciales disponibles para este ...