Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells
Carnosic acid (CA) and carnosol (CS) are two structurally related diterpenes present in rosemary herb (Rosmarinus officinalis). Although several studies have demonstrated that both diterpenes can scavenge free radicals and interfere in cellular processes such as cell proliferation, they may not nece...
Published in: | Molecular & Cellular Proteomics |
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American Society for Biochemistry and Molecular Biology
2017
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Online Access: | http://hdl.handle.net/10261/150540 https://doi.org/10.1074/mcp.M116.061481 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 |
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ftcsic:oai:digital.csic.es:10261/150540 2024-02-11T10:03:23+01:00 Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells Valdés, Alberto García-Cañas, Virginia Artemenko, Konstantin A. Simó, Carolina Bergquist, Jonas Cifuentes, Alejandro Ministerio de Economía y Competitividad (España) Comunidad de Madrid Swedish Research Council 2017 http://hdl.handle.net/10261/150540 https://doi.org/10.1074/mcp.M116.061481 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 unknown American Society for Biochemistry and Molecular Biology #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P S2013/ABI-2728/ALIBIRD-CM Postprint https://doi.org/10.1074/mcp.M116.061481 Sí doi:10.1074/mcp.M116.061481 e-issn: 1535-9484 issn: 1535-9476 Molecular and Cellular Proteomics 16(1): 8-22 (2017) http://hdl.handle.net/10261/150540 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 27834734 open Cancer biology Carnosic acid HT-29 cells Foodomics Dimethyl labeling Carnosol Colorectal cancer Mass spectrometry Proliferation Quantification artículo http://purl.org/coar/resource_type/c_6501 2017 ftcsic https://doi.org/10.1074/mcp.M116.06148110.13039/50110000332910.13039/100012818 2024-01-16T10:23:32Z Carnosic acid (CA) and carnosol (CS) are two structurally related diterpenes present in rosemary herb (Rosmarinus officinalis). Although several studies have demonstrated that both diterpenes can scavenge free radicals and interfere in cellular processes such as cell proliferation, they may not necessarily exert the same effects at the molecular level. In this work, a shotgun proteomics study based on stable isotope dimethyl labeling (DML) and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) has been performed to identify the relative changes in proteins and to gain some light on the specific molecular targets and mechanisms of action of CA and CS in HT-29 colon cancer cells. Protein profiles revealed that CA and CS induce different Nrf2-mediated response. Furthermore, examination of our data revealed that each diterpene affects protein homeostasis by different mechanisms. CA treatment induces the expression of proteins involved in the unfolded protein response in a concentration dependent manner reflecting ER stress, whereas CS directly inhibits chymotrypsin-like activity of the 20S proteasome. In conclusion, the unbiased proteomics-wide method applied in the present study has demonstrated to be a powerful tool to reveal differences on the mechanisms of action of two related bioactive compounds in the same biological model. This work was supported by the project AGL2014-53609-P (Ministerio de Economía y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economía y Competitividad for his FPI pre-doctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support. Peer Reviewed Article in Journal/Newspaper DML Digital.CSIC (Spanish National Research Council) Molecular & Cellular Proteomics 16 1 8 22 |
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Digital.CSIC (Spanish National Research Council) |
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Cancer biology Carnosic acid HT-29 cells Foodomics Dimethyl labeling Carnosol Colorectal cancer Mass spectrometry Proliferation Quantification |
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Cancer biology Carnosic acid HT-29 cells Foodomics Dimethyl labeling Carnosol Colorectal cancer Mass spectrometry Proliferation Quantification Valdés, Alberto García-Cañas, Virginia Artemenko, Konstantin A. Simó, Carolina Bergquist, Jonas Cifuentes, Alejandro Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
topic_facet |
Cancer biology Carnosic acid HT-29 cells Foodomics Dimethyl labeling Carnosol Colorectal cancer Mass spectrometry Proliferation Quantification |
description |
Carnosic acid (CA) and carnosol (CS) are two structurally related diterpenes present in rosemary herb (Rosmarinus officinalis). Although several studies have demonstrated that both diterpenes can scavenge free radicals and interfere in cellular processes such as cell proliferation, they may not necessarily exert the same effects at the molecular level. In this work, a shotgun proteomics study based on stable isotope dimethyl labeling (DML) and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) has been performed to identify the relative changes in proteins and to gain some light on the specific molecular targets and mechanisms of action of CA and CS in HT-29 colon cancer cells. Protein profiles revealed that CA and CS induce different Nrf2-mediated response. Furthermore, examination of our data revealed that each diterpene affects protein homeostasis by different mechanisms. CA treatment induces the expression of proteins involved in the unfolded protein response in a concentration dependent manner reflecting ER stress, whereas CS directly inhibits chymotrypsin-like activity of the 20S proteasome. In conclusion, the unbiased proteomics-wide method applied in the present study has demonstrated to be a powerful tool to reveal differences on the mechanisms of action of two related bioactive compounds in the same biological model. This work was supported by the project AGL2014-53609-P (Ministerio de Economía y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economía y Competitividad for his FPI pre-doctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support. Peer Reviewed |
author2 |
Ministerio de Economía y Competitividad (España) Comunidad de Madrid Swedish Research Council |
format |
Article in Journal/Newspaper |
author |
Valdés, Alberto García-Cañas, Virginia Artemenko, Konstantin A. Simó, Carolina Bergquist, Jonas Cifuentes, Alejandro |
author_facet |
Valdés, Alberto García-Cañas, Virginia Artemenko, Konstantin A. Simó, Carolina Bergquist, Jonas Cifuentes, Alejandro |
author_sort |
Valdés, Alberto |
title |
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
title_short |
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
title_full |
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
title_fullStr |
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
title_full_unstemmed |
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
title_sort |
nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells |
publisher |
American Society for Biochemistry and Molecular Biology |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/150540 https://doi.org/10.1074/mcp.M116.061481 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 |
genre |
DML |
genre_facet |
DML |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P S2013/ABI-2728/ALIBIRD-CM Postprint https://doi.org/10.1074/mcp.M116.061481 Sí doi:10.1074/mcp.M116.061481 e-issn: 1535-9484 issn: 1535-9476 Molecular and Cellular Proteomics 16(1): 8-22 (2017) http://hdl.handle.net/10261/150540 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 27834734 |
op_rights |
open |
op_doi |
https://doi.org/10.1074/mcp.M116.06148110.13039/50110000332910.13039/100012818 |
container_title |
Molecular & Cellular Proteomics |
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16 |
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22 |
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