Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites

• Published in: Molecules
• Main Authors: Virgen-Ortíz, José J., Pedrero, Sara G., Fernández-López, Laura, López-Carrobles, Nerea, Gorines, Beatriz C., Otero Hernández, Cristina, Fernández-Lafuente, Roberto
• Other Authors: Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Multidisciplinary Digital Publishing Institute 2017
• Subjects: Martínez; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10261/149339; https://doi.org/10.3390/molecules22010091; https://doi.org/10.13039/501100003329; https://doi.org/10.13039/501100003339

Lipases from Candida antarctica (isoform B) and Rhizomucor miehei (CALB and RML) have been immobilized on octyl-agarose (OC) and further coated with polyethylenimine (PEI) and dextran sulfate (DS). The enzymes just immobilized on OC supports could be easily released from the support using 2% SDS at...