Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis
SPARC/osteonectin is a multifunctional matricellular glycoprotein, which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during de...
Published in: | Gene |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier
2015
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/144447 https://doi.org/10.1016/j.gene.2015.05.036 |
id |
ftcsic:oai:digital.csic.es:10261/144447 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/144447 2024-02-11T10:08:27+01:00 Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis Torres-Núñez, Eva Suárez-Bregua, Paula Cal, Laura Cal, Rosa Cerdá-Reverter, José Miguel Rotllant, Josep 2015-09-01 http://hdl.handle.net/10261/144447 https://doi.org/10.1016/j.gene.2015.05.036 unknown Elsevier Postprint https://doi.org/10.1016/j.gene.2015.05.036 Sí issn: 1879-0038 Gene 568(2): 129-139 (2015) http://hdl.handle.net/10261/144447 doi:10.1016/j.gene.2015.05.036 none Sparc Post-embryonic development Metamorphosis Gene expression Turbot Osteonectin artículo http://purl.org/coar/resource_type/c_6501 2015 ftcsic https://doi.org/10.1016/j.gene.2015.05.036 2024-01-16T10:20:42Z SPARC/osteonectin is a multifunctional matricellular glycoprotein, which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during development are largely unknown. In this report, we describe the isolation, characterization, post-embryonic developmental expression and environmental thermal regulation of sparc in turbot. The full-length turbot sparc cDNA contains 930. bp and encodes a protein of 310 amino acids, which shares 77, 75 and 80% identity with human, frog and zebrafish, respectively. Results of whole-mount in situ hybridization reveal a dynamic expression profile during post-embryonic turbot development. Sparc is expressed differentially in the cranioencephalic region; mainly in jaws, branchial arches, fin folds and rays of caudal, dorsal and anal fins. Furthermore, ontogenetic studies demonstrated that Sparc gene expression is dynamically regulated during post-embryonic turbot development, with high expression during stage-specific post-embryonic remodeling. Additionally, the effect of thermal environmental conditions on turbot development and on ontogenetic sparc expression was evaluated. © 2015 Elsevier B.V. Peer Reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Gene 568 2 129 139 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Sparc Post-embryonic development Metamorphosis Gene expression Turbot Osteonectin |
spellingShingle |
Sparc Post-embryonic development Metamorphosis Gene expression Turbot Osteonectin Torres-Núñez, Eva Suárez-Bregua, Paula Cal, Laura Cal, Rosa Cerdá-Reverter, José Miguel Rotllant, Josep Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
topic_facet |
Sparc Post-embryonic development Metamorphosis Gene expression Turbot Osteonectin |
description |
SPARC/osteonectin is a multifunctional matricellular glycoprotein, which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during development are largely unknown. In this report, we describe the isolation, characterization, post-embryonic developmental expression and environmental thermal regulation of sparc in turbot. The full-length turbot sparc cDNA contains 930. bp and encodes a protein of 310 amino acids, which shares 77, 75 and 80% identity with human, frog and zebrafish, respectively. Results of whole-mount in situ hybridization reveal a dynamic expression profile during post-embryonic turbot development. Sparc is expressed differentially in the cranioencephalic region; mainly in jaws, branchial arches, fin folds and rays of caudal, dorsal and anal fins. Furthermore, ontogenetic studies demonstrated that Sparc gene expression is dynamically regulated during post-embryonic turbot development, with high expression during stage-specific post-embryonic remodeling. Additionally, the effect of thermal environmental conditions on turbot development and on ontogenetic sparc expression was evaluated. © 2015 Elsevier B.V. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Torres-Núñez, Eva Suárez-Bregua, Paula Cal, Laura Cal, Rosa Cerdá-Reverter, José Miguel Rotllant, Josep |
author_facet |
Torres-Núñez, Eva Suárez-Bregua, Paula Cal, Laura Cal, Rosa Cerdá-Reverter, José Miguel Rotllant, Josep |
author_sort |
Torres-Núñez, Eva |
title |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_short |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_full |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_fullStr |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_full_unstemmed |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_sort |
molecular cloning and characterization of the matricellular protein sparc/osteonectin in flatfish, scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/10261/144447 https://doi.org/10.1016/j.gene.2015.05.036 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Postprint https://doi.org/10.1016/j.gene.2015.05.036 Sí issn: 1879-0038 Gene 568(2): 129-139 (2015) http://hdl.handle.net/10261/144447 doi:10.1016/j.gene.2015.05.036 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.gene.2015.05.036 |
container_title |
Gene |
container_volume |
568 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
139 |
_version_ |
1790607784534867968 |