Stabilization of Candida antarctica Lipase B (CALB) Immobilized on Octyl Agarose by Treatment with Polyethyleneimine (PEI)

• Published in: Molecules
• Main Authors: Peirce, Sara, Tacias-Pascacio, Veymar G., Russo, María Elena, Marzocchella, Antonio, Virgen-Ortíz, José J., Fernández-Lafuente, Roberto
• Other Authors: Ministerio de Economía y Competitividad (España), Università degli Studi di Napoli Federico II, Consejo Nacional de Ciencia y Tecnología (México), Consejo Superior de Investigaciones Científicas (España)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Multidisciplinary Digital Publishing Institute 2016
• Subjects: Triton; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10261/142084; https://doi.org/10.3390/molecules21060751; https://doi.org/10.13039/501100003329; https://doi.org/10.13039/100007195; https://doi.org/10.13039/501100003141; https://doi.org/10.13039/501100003339

Lipase B from Candida antarctica (CALB) was immobilized on octyl agarose (OC) and physically modified with polyethyleneimine (PEI) in order to confer a strong ion exchange character to the enzyme and thus enable the immobilization of other enzymes on its surface. The enzyme activity was fully mainta...