Coxiella burnetii genotypes in Iberian wildlife

• Published in: Microbial Ecology
• Main Authors: González-Barrio, David, Hagen, Ferry, Tilburg, Jeroen JHC, Ruiz Fons, Francisco
• Other Authors: Universidad de Castilla La Mancha, European Commission, Ministerio de Economía y Competitividad (España)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Springer 2016
• Subjects: Q fever; MLVA; Molecular ecology; Zoonoses; Rattus rattus
• Online Access: http://hdl.handle.net/10261/141518; https://doi.org/10.1007/s00248-016-0786-9; https://doi.org/10.13039/501100007480; https://doi.org/10.13039/501100000780; https://doi.org/10.13039/501100003329

To investigate if Coxiella burnetii, the causative agent of Q fever, genotypes circulating in wildlife are associated with those infecting livestock and humans, multiple-locus variable number tandem-repeat analysis (MLVA-6-marker) was carried out over C. burnetii obtained from red deer (Cervus elaphus), Eurasian wild boar (Sus scrofa), European wild rabbit (Oryctolagus cuniculus), black rat (Rattus rattus), and wood mouse (Apodemus sylvaticus). MLVA typing was performed by using six variable loci in C. burnetii: Ms23, Ms24, Ms27, Ms28, Ms33, and Ms34. The C. burnetii cooperative database from MLVABank 5.0 was employed to compare genotypes found in this study with 344 isolates of diverse origin. Twenty-two genotypes from wildlife and two genotypes from domestic goats were identified. Some MLVA genotypes identified in wildlife or in farmed game clustered with genotypes of human Q fever clinical cases, supporting the idea that humans and wildlife share C. burnetii genotypes. The major part of genotypes identified in coexisting red deer and rabbits clustered according to their host of origin, suggesting host specificity for particular C. burnetii genotypes. These findings provide important insights to understand the epidemiology of C. burnetii at the wildlife-livestock-human interface. This work was funded by EU FP7 Grant ANTIGONE (278976) and CDTI (Centro para el Desarrollo Tecnológico Industrial, Spanish Ministry for Economy and Competitiveness MINECO). F.R-F is supported by the Ramón y Cajal program of the Spanish Ministry for the Economy and Competitiveness, and D.G-B acknowledges funding by Cátedra UCLM-Fundación ENRESA. Peer Reviewed