Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development
21 pages, 6 figures, supporting information https://dx.doi.org/10.1371/journal.pone.0154592 AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQ...
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ftcsic:oai:digital.csic.es:10261/134650 2024-02-11T10:02:13+01:00 Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development Chauvigné, François Fjelldal, Per Gunnar Cerdà, Joan Finn, Roderick N. Research Council of Norway Ministerio de Economía y Competitividad (España) 2016-05 http://hdl.handle.net/10261/134650 https://doi.org/10.1371/journal.pone.0154592 https://doi.org/10.13039/501100003329 unknown Public Library of Science Publisher's version https://dx.doi.org/10.1371/journal.pone.0154592 Sí doi:10.1371/journal.pone.0154592 issn: 1932-6203 e-issn: 1932-6203 PLoS ONE 11(5): e0154592 (2016) http://hdl.handle.net/10261/134650 http://dx.doi.org/10.13039/501100003329 27153052 open artículo http://purl.org/coar/resource_type/c_6501 2016 ftcsic https://doi.org/10.1371/journal.pone.015459210.13039/501100003329 2024-01-16T10:16:26Z 21 pages, 6 figures, supporting information https://dx.doi.org/10.1371/journal.pone.0154592 AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQP0 has a low intrinsic water permeability, but can form cell-to-cell junctions between the lens fibers. It is not known whether the adhesive properties of AQP0 is a derived feature found only in mammals, or exists as a conserved ancestral trait in non-mammalian vertebrates. Here we show that a tetraploid teleost, the Atlantic salmon, expresses four Aqp0 paralogs in the developing lens, but also expresses significant levels of aqp0 mRNAs and proteins in the epithelia of the pronephros, presumptive enterocytes, gill filament and epidermis. Quantitative PCR reveals that aqp0 mRNA titres increase by three orders of magnitude between the onset of somitogenesis and pigmentation of the eye. Using in situ hybridization and specific antisera, we show that at least two of the channels (Aqp0a1, -0b1 and/or -0b2) are localized in the extraocular basolateral and apical membranes, while Aqp0a2 is lens-specific. Heterologous expression of the Aqp0 paralogs in adhesion-deficient mouse fibolast L-cells reveals that, as for human AQP0, each intact salmon channel retains cell-to-cell adhesive properties. The strongest Aqp0 interactions are auto-adhesion, suggesting that homo-octamers likely form the intercellular junctions of the developing lens and epithelial tissues. The present data are thus the first to show the adhesion potential of Aqp0 channels in a non-mammalian vertebrate, and further uncover a novel extraocular role of the channels during vertebrate development This work was supported by the Research Council of Norway (RCN) projects 224816/E40 and 254872/E40 to RNF, the Spanish Ministry of Economy and Competitiveness (MINECO) grant no. AGL2013-41196-R to JC. FC was supported by postdoctoral fellowships from the RCN ... Article in Journal/Newspaper Atlantic salmon Digital.CSIC (Spanish National Research Council) Norway PLOS ONE 11 5 e0154592 |
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21 pages, 6 figures, supporting information https://dx.doi.org/10.1371/journal.pone.0154592 AQP0 water channels are the most abundant proteins expressed in the mammalian lens fiber membranes where they are essential for lens development and transparency. Unlike other aquaporin paralogs, mammalian AQP0 has a low intrinsic water permeability, but can form cell-to-cell junctions between the lens fibers. It is not known whether the adhesive properties of AQP0 is a derived feature found only in mammals, or exists as a conserved ancestral trait in non-mammalian vertebrates. Here we show that a tetraploid teleost, the Atlantic salmon, expresses four Aqp0 paralogs in the developing lens, but also expresses significant levels of aqp0 mRNAs and proteins in the epithelia of the pronephros, presumptive enterocytes, gill filament and epidermis. Quantitative PCR reveals that aqp0 mRNA titres increase by three orders of magnitude between the onset of somitogenesis and pigmentation of the eye. Using in situ hybridization and specific antisera, we show that at least two of the channels (Aqp0a1, -0b1 and/or -0b2) are localized in the extraocular basolateral and apical membranes, while Aqp0a2 is lens-specific. Heterologous expression of the Aqp0 paralogs in adhesion-deficient mouse fibolast L-cells reveals that, as for human AQP0, each intact salmon channel retains cell-to-cell adhesive properties. The strongest Aqp0 interactions are auto-adhesion, suggesting that homo-octamers likely form the intercellular junctions of the developing lens and epithelial tissues. The present data are thus the first to show the adhesion potential of Aqp0 channels in a non-mammalian vertebrate, and further uncover a novel extraocular role of the channels during vertebrate development This work was supported by the Research Council of Norway (RCN) projects 224816/E40 and 254872/E40 to RNF, the Spanish Ministry of Economy and Competitiveness (MINECO) grant no. AGL2013-41196-R to JC. FC was supported by postdoctoral fellowships from the RCN ... |
author2 |
Research Council of Norway Ministerio de Economía y Competitividad (España) |
format |
Article in Journal/Newspaper |
author |
Chauvigné, François Fjelldal, Per Gunnar Cerdà, Joan Finn, Roderick N. |
spellingShingle |
Chauvigné, François Fjelldal, Per Gunnar Cerdà, Joan Finn, Roderick N. Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
author_facet |
Chauvigné, François Fjelldal, Per Gunnar Cerdà, Joan Finn, Roderick N. |
author_sort |
Chauvigné, François |
title |
Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
title_short |
Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
title_full |
Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
title_fullStr |
Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
title_full_unstemmed |
Auto-Adhesion Potential of Extraocular Aqp0 during Teleost Development |
title_sort |
auto-adhesion potential of extraocular aqp0 during teleost development |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/134650 https://doi.org/10.1371/journal.pone.0154592 https://doi.org/10.13039/501100003329 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Publisher's version https://dx.doi.org/10.1371/journal.pone.0154592 Sí doi:10.1371/journal.pone.0154592 issn: 1932-6203 e-issn: 1932-6203 PLoS ONE 11(5): e0154592 (2016) http://hdl.handle.net/10261/134650 http://dx.doi.org/10.13039/501100003329 27153052 |
op_rights |
open |
op_doi |
https://doi.org/10.1371/journal.pone.015459210.13039/501100003329 |
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PLOS ONE |
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11 |
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5 |
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e0154592 |
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