Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of but...
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Molecular Diversity Preservation International
2014
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Online Access: | http://hdl.handle.net/10261/130914 https://doi.org/10.3390/molecules19079562 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100003593 |
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ftcsic:oai:digital.csic.es:10261/130914 2024-02-11T09:57:36+01:00 Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB Alves, Joana S. García-Galán, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernández-Lafuente, Roberto Rodrigues, Rafael C. Consejo Superior de Investigaciones Científicas (España) Ministerio de Economía y Competitividad (España) Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) 2014-07-07 http://hdl.handle.net/10261/130914 https://doi.org/10.3390/molecules19079562 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100003593 unknown Molecular Diversity Preservation International Publisher's version http://dx.doi.org/10.3390/molecules19079562 Sí issn: 1420-3049 Molecules 19(7): 9562-9576 (2014) http://hdl.handle.net/10261/130914 doi:10.3390/molecules19079562 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003593 25004067 open esterification Butyl acetate enzyme stability enzyme reuse ultrasound lipase artículo http://purl.org/coar/resource_type/c_6501 2014 ftcsic https://doi.org/10.3390/molecules1907956210.13039/50110000333910.13039/50110000332910.13039/501100003593 2024-01-16T10:14:43Z It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: Temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol: Acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. This work was supported by grants from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and CTQ2013-41507-R from Spanish MINECO. We would like to thank Novozymes Spain for its comprehensive support of this research, in special Ramiro Martínez (Novozymes, Spain) for kindly supplying the enzymes used in the work. We also thank CNPq (Brazil) for a fellowship to A.M. Silva and FAPERGS (Brazil) for a fellowship to J.S. Alves. A Ph D. fellowship from Spanish Goberment to Miss Garcia-Galan is also acknowledged The help and suggestions from Ángel Berenguer-Murcia (Instituto de Materiales, Universidad de Alicante) are gratefully recognized. We acknowledge support by the CSIC Open Access ... Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Martínez ENVELOPE(-62.183,-62.183,-64.650,-64.650) Molecules 19 7 9562 9576 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
esterification Butyl acetate enzyme stability enzyme reuse ultrasound lipase |
spellingShingle |
esterification Butyl acetate enzyme stability enzyme reuse ultrasound lipase Alves, Joana S. García-Galán, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernández-Lafuente, Roberto Rodrigues, Rafael C. Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
topic_facet |
esterification Butyl acetate enzyme stability enzyme reuse ultrasound lipase |
description |
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: Temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol: Acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. This work was supported by grants from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and CTQ2013-41507-R from Spanish MINECO. We would like to thank Novozymes Spain for its comprehensive support of this research, in special Ramiro Martínez (Novozymes, Spain) for kindly supplying the enzymes used in the work. We also thank CNPq (Brazil) for a fellowship to A.M. Silva and FAPERGS (Brazil) for a fellowship to J.S. Alves. A Ph D. fellowship from Spanish Goberment to Miss Garcia-Galan is also acknowledged The help and suggestions from Ángel Berenguer-Murcia (Instituto de Materiales, Universidad de Alicante) are gratefully recognized. We acknowledge support by the CSIC Open Access ... |
author2 |
Consejo Superior de Investigaciones Científicas (España) Ministerio de Economía y Competitividad (España) Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) |
format |
Article in Journal/Newspaper |
author |
Alves, Joana S. García-Galán, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernández-Lafuente, Roberto Rodrigues, Rafael C. |
author_facet |
Alves, Joana S. García-Galán, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernández-Lafuente, Roberto Rodrigues, Rafael C. |
author_sort |
Alves, Joana S. |
title |
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
title_short |
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
title_full |
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
title_fullStr |
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
title_full_unstemmed |
Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB |
title_sort |
combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by calb |
publisher |
Molecular Diversity Preservation International |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/130914 https://doi.org/10.3390/molecules19079562 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100003593 |
long_lat |
ENVELOPE(-62.183,-62.183,-64.650,-64.650) |
geographic |
Martínez |
geographic_facet |
Martínez |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Publisher's version http://dx.doi.org/10.3390/molecules19079562 Sí issn: 1420-3049 Molecules 19(7): 9562-9576 (2014) http://hdl.handle.net/10261/130914 doi:10.3390/molecules19079562 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003593 25004067 |
op_rights |
open |
op_doi |
https://doi.org/10.3390/molecules1907956210.13039/50110000333910.13039/50110000332910.13039/501100003593 |
container_title |
Molecules |
container_volume |
19 |
container_issue |
7 |
container_start_page |
9562 |
op_container_end_page |
9576 |
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1790593138936512512 |