Comprehensive transcriptomic analysis of the process of gonadal sex differentiation in the turbot (Scophthalmus maximus)

• Published in: Molecular and Cellular Endocrinology
• Main Authors: Ribas, Laia, Robledo, Diego, Gómez-Tato, Antonio, Viñas, Ana, Martínez, Paulino, Piferrer, Francesc
• Other Authors: Ministerio de Educación, Cultura y Deporte (España)
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier 2016
• Subjects: Gonad development; Transcriptomes; Sex differentiation; Novel genes; Sex and growth QTL; QTL; Scophthalmus maximus; Turbot
• Online Access: http://hdl.handle.net/10261/130855; https://doi.org/10.1016/j.mce.2015.11.006; https://doi.org/10.13039/501100003176

18 pages, 7 figures, 2 tables, 1 appendix supplementary data http://dx.doi.org/10.1016/j.mce.2015.11.006 The turbot is a flatfish with a ZW/ZZ sex determination system but with a still unknown sex determining gene(s), and with a marked sexual growth dimorphism in favor of females. To better understand sexual development in turbot we sampled young turbot encompassing the whole process of gonadal differentiation and conducted a comprehensive transcriptomic study on its sex differentiation using a validated custom oligomicroarray. Also, the expression profiles of 18 canonical reproduction-related genes were studied along gonad development. The expression levels of gonadal aromatase cyp19a1a alone at three months of age allowed the accurate and early identification of sex before the first signs of histological differentiation. A total of 56 differentially expressed genes (DEG) that had not previously been related to sex differentiation in fish were identified within the first three months of age, of which 44 were associated with ovarian differentiation (e.g., cd98, gpd1 and cry2), and 12 with testicular differentiation (e.g., ace, capn8 and nxph1). To identify putative sex determining genes, ∼4.000 DEG in juvenile gonads were mapped and their positions compared with that of previously identified sex- and growth-related quantitative trait loci (QTL). Although no genes mapped to the previously identified sex-related QTLs, two genes (foxl2 and 17βhsd) of the canonical reproduction-related genes mapped to growth-QTLs in linkage group (LG) 15 and LG6, respectively, suggesting that these genes are related to the growth dimorphism in this species Work supported by Spanish Government Consolider-Ingenio 2010 grant “Aquagenomics” (ref. CDS2007-0002) to PM and FP. LR was supported by Aquagenomics and EpigenAqua postdoctoral contract; DR was supported by a FPU fellowship from the Ministerio de Educación Cultura y Deporte of the Spanish Government Peer Reviewed