Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)
Special issue How changes in ice cover, permafrost and UV radiation impact on biodiversity and biogeochemical fluxes in the Arctic Ocean – The Malina project.-- 13 pages, 6 figures, 4 tables, supplementary material related to this article is available online at: http://www.biogeosciences.net/10/2747...
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ftcsic:oai:digital.csic.es:10261/115546 2024-02-11T10:00:54+01:00 Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) Ortega-Retuerta, E. Joux, Fabien Jeffrey, W.H. Ghiglione, J. F. 2013-04-25 http://hdl.handle.net/10261/115546 https://doi.org/10.5194/bg-10-2747-2013 unknown European Geosciences Union https://doi.org/10.5194/bg-10-2747-2013 issn: 1726-4170 e-issn: 1726-4189 Biogeosciences 10: 2747-2759 (2013) http://hdl.handle.net/10261/115546 doi:10.5194/bg-10-2747-2013 open artículo http://purl.org/coar/resource_type/c_6501 2013 ftcsic https://doi.org/10.5194/bg-10-2747-2013 2024-01-16T10:07:27Z Special issue How changes in ice cover, permafrost and UV radiation impact on biodiversity and biogeochemical fluxes in the Arctic Ocean – The Malina project.-- 13 pages, 6 figures, 4 tables, supplementary material related to this article is available online at: http://www.biogeosciences.net/10/2747/2013/bg-10-2747-2013-supplement.pdf We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis-single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7 % similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river ... Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea Ice Mackenzie river permafrost Digital.CSIC (Spanish National Research Council) Arctic Arctic Ocean Mackenzie River Biogeosciences 10 4 2747 2759 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
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unknown |
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Special issue How changes in ice cover, permafrost and UV radiation impact on biodiversity and biogeochemical fluxes in the Arctic Ocean – The Malina project.-- 13 pages, 6 figures, 4 tables, supplementary material related to this article is available online at: http://www.biogeosciences.net/10/2747/2013/bg-10-2747-2013-supplement.pdf We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis-single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7 % similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river ... |
format |
Article in Journal/Newspaper |
author |
Ortega-Retuerta, E. Joux, Fabien Jeffrey, W.H. Ghiglione, J. F. |
spellingShingle |
Ortega-Retuerta, E. Joux, Fabien Jeffrey, W.H. Ghiglione, J. F. Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
author_facet |
Ortega-Retuerta, E. Joux, Fabien Jeffrey, W.H. Ghiglione, J. F. |
author_sort |
Ortega-Retuerta, E. |
title |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_short |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_fullStr |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full_unstemmed |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_sort |
spatial variability of particle-attached and free-living bacterial diversity in surface waters from the mackenzie river to the beaufort sea (canadian arctic) |
publisher |
European Geosciences Union |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/115546 https://doi.org/10.5194/bg-10-2747-2013 |
geographic |
Arctic Arctic Ocean Mackenzie River |
geographic_facet |
Arctic Arctic Ocean Mackenzie River |
genre |
Arctic Arctic Ocean Beaufort Sea Ice Mackenzie river permafrost |
genre_facet |
Arctic Arctic Ocean Beaufort Sea Ice Mackenzie river permafrost |
op_relation |
https://doi.org/10.5194/bg-10-2747-2013 issn: 1726-4170 e-issn: 1726-4189 Biogeosciences 10: 2747-2759 (2013) http://hdl.handle.net/10261/115546 doi:10.5194/bg-10-2747-2013 |
op_rights |
open |
op_doi |
https://doi.org/10.5194/bg-10-2747-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
4 |
container_start_page |
2747 |
op_container_end_page |
2759 |
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1790596625388797952 |