The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua)
Fillets of Atlantic cod (Gadus morhua) were held for 36 h in brines of pH 6.5 and 8.5, respectively, containing various combinations of NaCl, KCl, MgCl2 and CaCl2. Proximate analyses and chloride content were determined. Soluble muscle protein extracted in distilled water and protein released into t...
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Online Access: | http://hdl.handle.net/10261/102059 https://doi.org/10.1016/j.foodchem.2004.04.049 |
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ftcsic:oai:digital.csic.es:10261/102059 2024-02-11T10:01:58+01:00 The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) Martínez Álvarez, Óscar Gómez Guillén, M. C. 2005 http://hdl.handle.net/10261/102059 https://doi.org/10.1016/j.foodchem.2004.04.049 unknown Elsevier doi:10.1016/j.foodchem.2004.04.049 issn: 0308-8146 Food Chemistry 92: 71- 77 (2005) http://hdl.handle.net/10261/102059 none Brined cod Brines Protein solubility pH Divalent salts artículo http://purl.org/coar/resource_type/c_6501 2005 ftcsic https://doi.org/10.1016/j.foodchem.2004.04.049 2024-01-16T10:01:11Z Fillets of Atlantic cod (Gadus morhua) were held for 36 h in brines of pH 6.5 and 8.5, respectively, containing various combinations of NaCl, KCl, MgCl2 and CaCl2. Proximate analyses and chloride content were determined. Soluble muscle protein extracted in distilled water and protein released into the brine following salting was determined by SDS-PAGE. The soluble fraction was not affected by the presence of divalent salts or KCl in the brines, but it was affected by the initial pH. Both actin and myosin heavy chain (MHC) were released in pH 6.5 brines after immersion of the fish. On the other hand, an initial pH of 8.5 did not favour the release of these proteins. There was a smaller variety of remaining soluble proteins in salted muscle with an initial pH of 6.5, than with 8.5. However, this effect of the lower pH was offset to the extent that the ionic strength of the brine was higher. © 2004 Elsevier Ltd. All rights reserved. Peer Reviewed Article in Journal/Newspaper atlantic cod Gadus morhua Digital.CSIC (Spanish National Research Council) Food Chemistry 92 1 71 77 |
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Digital.CSIC (Spanish National Research Council) |
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topic |
Brined cod Brines Protein solubility pH Divalent salts |
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Brined cod Brines Protein solubility pH Divalent salts Martínez Álvarez, Óscar Gómez Guillén, M. C. The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
topic_facet |
Brined cod Brines Protein solubility pH Divalent salts |
description |
Fillets of Atlantic cod (Gadus morhua) were held for 36 h in brines of pH 6.5 and 8.5, respectively, containing various combinations of NaCl, KCl, MgCl2 and CaCl2. Proximate analyses and chloride content were determined. Soluble muscle protein extracted in distilled water and protein released into the brine following salting was determined by SDS-PAGE. The soluble fraction was not affected by the presence of divalent salts or KCl in the brines, but it was affected by the initial pH. Both actin and myosin heavy chain (MHC) were released in pH 6.5 brines after immersion of the fish. On the other hand, an initial pH of 8.5 did not favour the release of these proteins. There was a smaller variety of remaining soluble proteins in salted muscle with an initial pH of 6.5, than with 8.5. However, this effect of the lower pH was offset to the extent that the ionic strength of the brine was higher. © 2004 Elsevier Ltd. All rights reserved. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Martínez Álvarez, Óscar Gómez Guillén, M. C. |
author_facet |
Martínez Álvarez, Óscar Gómez Guillén, M. C. |
author_sort |
Martínez Álvarez, Óscar |
title |
The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
title_short |
The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
title_full |
The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
title_fullStr |
The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
title_full_unstemmed |
The effect of brine composition and pH on the yield and nature of water-soluble proteins extractable from brined muscle of cod (Gadus morhua) |
title_sort |
effect of brine composition and ph on the yield and nature of water-soluble proteins extractable from brined muscle of cod (gadus morhua) |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://hdl.handle.net/10261/102059 https://doi.org/10.1016/j.foodchem.2004.04.049 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.1016/j.foodchem.2004.04.049 issn: 0308-8146 Food Chemistry 92: 71- 77 (2005) http://hdl.handle.net/10261/102059 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.foodchem.2004.04.049 |
container_title |
Food Chemistry |
container_volume |
92 |
container_issue |
1 |
container_start_page |
71 |
op_container_end_page |
77 |
_version_ |
1790597866429874176 |