Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.)
Sperm samples may be used for assisted reproductive technologies (e.g., farmed or endangered species) or as a source of haploid DNA or sperm-specific RNA. When ejaculated spermatozoa are not available or are very difficult to obtain, as is the case for most wild endangered species, the epididymides...
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Online Access: | http://hdl.handle.net/10261/101693 https://doi.org/10.1016/j.theriogenology.2014.04.029 |
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ftcsic:oai:digital.csic.es:10261/101693 2024-02-11T10:02:45+01:00 Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) Muñoz-Fuentes, Violeta Kinde Forsberg, C. Vilà, Carles Morrell, Jane M. 2014 http://hdl.handle.net/10261/101693 https://doi.org/10.1016/j.theriogenology.2014.04.029 unknown Elsevier doi:10.1016/j.theriogenology.2014.04.029 issn: 0093-691X Theriogenology 82: 773- 776 (2014) http://hdl.handle.net/10261/101693 none Single-cell DNA Colloid RNA Sperm genotyping Assisted reproductive technologies Androcoll-C artículo http://purl.org/coar/resource_type/c_6501 2014 ftcsic https://doi.org/10.1016/j.theriogenology.2014.04.029 2024-01-16T10:00:59Z Sperm samples may be used for assisted reproductive technologies (e.g., farmed or endangered species) or as a source of haploid DNA or sperm-specific RNA. When ejaculated spermatozoa are not available or are very difficult to obtain, as is the case for most wild endangered species, the epididymides of dead animals (e.g., animals that have been found dead, shot by hunters or poachers, or that that require euthanasia in zoological collections) can be used as a source of sperm. Such epididymal sperm samples are usually contaminated with cellular debris, erythrocytes, leukocytes, and sometimes also bacteria. These contaminants may be sources of reactive oxygen species that damage spermatozoa during freezing or contribute undesired genetic material from diploid cells. We used single-layer centrifugation through a colloid formulation, Androcoll-C, to successfully separate wolf epididymal spermatozoa from contaminating cells and cellular debris in epididymal samples harvested from carcasses. Such a procedure may potentially be applied to epididymal sperm samples from other species. © 2014 Elsevier Inc. Peer Reviewed Article in Journal/Newspaper Canis lupus Digital.CSIC (Spanish National Research Council) Theriogenology 82 5 773 776 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Single-cell DNA Colloid RNA Sperm genotyping Assisted reproductive technologies Androcoll-C |
spellingShingle |
Single-cell DNA Colloid RNA Sperm genotyping Assisted reproductive technologies Androcoll-C Muñoz-Fuentes, Violeta Kinde Forsberg, C. Vilà, Carles Morrell, Jane M. Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
topic_facet |
Single-cell DNA Colloid RNA Sperm genotyping Assisted reproductive technologies Androcoll-C |
description |
Sperm samples may be used for assisted reproductive technologies (e.g., farmed or endangered species) or as a source of haploid DNA or sperm-specific RNA. When ejaculated spermatozoa are not available or are very difficult to obtain, as is the case for most wild endangered species, the epididymides of dead animals (e.g., animals that have been found dead, shot by hunters or poachers, or that that require euthanasia in zoological collections) can be used as a source of sperm. Such epididymal sperm samples are usually contaminated with cellular debris, erythrocytes, leukocytes, and sometimes also bacteria. These contaminants may be sources of reactive oxygen species that damage spermatozoa during freezing or contribute undesired genetic material from diploid cells. We used single-layer centrifugation through a colloid formulation, Androcoll-C, to successfully separate wolf epididymal spermatozoa from contaminating cells and cellular debris in epididymal samples harvested from carcasses. Such a procedure may potentially be applied to epididymal sperm samples from other species. © 2014 Elsevier Inc. Peer Reviewed |
format |
Article in Journal/Newspaper |
author |
Muñoz-Fuentes, Violeta Kinde Forsberg, C. Vilà, Carles Morrell, Jane M. |
author_facet |
Muñoz-Fuentes, Violeta Kinde Forsberg, C. Vilà, Carles Morrell, Jane M. |
author_sort |
Muñoz-Fuentes, Violeta |
title |
Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
title_short |
Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
title_full |
Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
title_fullStr |
Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
title_full_unstemmed |
Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.) |
title_sort |
single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, canis lupus (l.) |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/101693 https://doi.org/10.1016/j.theriogenology.2014.04.029 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
doi:10.1016/j.theriogenology.2014.04.029 issn: 0093-691X Theriogenology 82: 773- 776 (2014) http://hdl.handle.net/10261/101693 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.theriogenology.2014.04.029 |
container_title |
Theriogenology |
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82 |
container_issue |
5 |
container_start_page |
773 |
op_container_end_page |
776 |
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1790598807974576128 |