Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil
Three different lipases (from Candida antarctica fraction B (CALB), Thermomyces lanuginose (TLL), and Rhizomucor miehei (RML)) were immobilized by two different methods, immobilization on CNBr-activated Sepharose via a mild covalent immobilization or adsorption onto hydrophobic supports (Octyl-Sepha...
Published in: | Journal of the American Oil Chemists' Society |
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Online Access: | http://hdl.handle.net/10261/101379 https://doi.org/10.1007/s11746-011-1885-x https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 |
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ftcsic:oai:digital.csic.es:10261/101379 2024-02-11T09:57:19+01:00 Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil Fernández-Lorente, Gloria Betancor, Lorena Carrascosa, Alfonso V. Palomo, José Miguel Guisán, José Manuel Ministerio de Ciencia e Innovación (España) European Commission Consejo Superior de Investigaciones Científicas (España) 2012 http://hdl.handle.net/10261/101379 https://doi.org/10.1007/s11746-011-1885-x https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 unknown Springer doi:10.1007/s11746-011-1885-x issn: 0003-021X e-issn: 1558-9331 Journal of the American Oil Chemists' Society 89(1): 97-102 (2012) http://hdl.handle.net/10261/101379 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 none Lipases Chemical modification EPA DHA Omega-3 fatty acids artículo http://purl.org/coar/resource_type/c_6501 2012 ftcsic https://doi.org/10.1007/s11746-011-1885-x10.13039/50110000483710.13039/50110000078010.13039/501100003339 2024-01-16T10:00:52Z Three different lipases (from Candida antarctica fraction B (CALB), Thermomyces lanuginose (TLL), and Rhizomucor miehei (RML)) were immobilized by two different methods, immobilization on CNBr-activated Sepharose via a mild covalent immobilization or adsorption onto hydrophobic supports (Octyl-Sepharose). These immobilized preparations were chemically and physically modified on the protein surface (enzyme carboxylic groups with ethylenediamine, amino groups with succinic anhydride, or coating with polyethyleneimine).The activity and selectivity in the production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by enzymatic hydrolysis of sardine oil were evaluated. Activity and selectivity were dependent on the different lipases, the immobilization protocols, the modification methods, and the pH of the reaction media. The selectivity (EPA/DHA ratio) of RML immobilized on CNBr-activated Sepharose was increased after succinylation from 7.5 to 34 at pH 6.0. The selectivity of octyl-RML improved from 1.5 to 8.5 when pH was increased from 6 to 8. The selectivity and activity of octyl-TLL increased twofold after PEI coating at pH 6. The properties of CAL-B derivatives were slightly altered after modification. © 2011 AOCS. This work was sponsored by the Spanish Ministry of Science and Innovation (AGL-2009-07526) and the Intramural project (200980I133). We gratefully recognize the Spanish Ministry of Science and Innovation for the ‘‘Ramón y Cajal’’ contract for Dr. Fernandez-Lorente. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Fernandez ENVELOPE(-62.233,-62.233,-63.250,-63.250) Journal of the American Oil Chemists' Society 89 1 97 102 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Lipases Chemical modification EPA DHA Omega-3 fatty acids |
spellingShingle |
Lipases Chemical modification EPA DHA Omega-3 fatty acids Fernández-Lorente, Gloria Betancor, Lorena Carrascosa, Alfonso V. Palomo, José Miguel Guisán, José Manuel Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
topic_facet |
Lipases Chemical modification EPA DHA Omega-3 fatty acids |
description |
Three different lipases (from Candida antarctica fraction B (CALB), Thermomyces lanuginose (TLL), and Rhizomucor miehei (RML)) were immobilized by two different methods, immobilization on CNBr-activated Sepharose via a mild covalent immobilization or adsorption onto hydrophobic supports (Octyl-Sepharose). These immobilized preparations were chemically and physically modified on the protein surface (enzyme carboxylic groups with ethylenediamine, amino groups with succinic anhydride, or coating with polyethyleneimine).The activity and selectivity in the production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by enzymatic hydrolysis of sardine oil were evaluated. Activity and selectivity were dependent on the different lipases, the immobilization protocols, the modification methods, and the pH of the reaction media. The selectivity (EPA/DHA ratio) of RML immobilized on CNBr-activated Sepharose was increased after succinylation from 7.5 to 34 at pH 6.0. The selectivity of octyl-RML improved from 1.5 to 8.5 when pH was increased from 6 to 8. The selectivity and activity of octyl-TLL increased twofold after PEI coating at pH 6. The properties of CAL-B derivatives were slightly altered after modification. © 2011 AOCS. This work was sponsored by the Spanish Ministry of Science and Innovation (AGL-2009-07526) and the Intramural project (200980I133). We gratefully recognize the Spanish Ministry of Science and Innovation for the ‘‘Ramón y Cajal’’ contract for Dr. Fernandez-Lorente. Peer Reviewed |
author2 |
Ministerio de Ciencia e Innovación (España) European Commission Consejo Superior de Investigaciones Científicas (España) |
format |
Article in Journal/Newspaper |
author |
Fernández-Lorente, Gloria Betancor, Lorena Carrascosa, Alfonso V. Palomo, José Miguel Guisán, José Manuel |
author_facet |
Fernández-Lorente, Gloria Betancor, Lorena Carrascosa, Alfonso V. Palomo, José Miguel Guisán, José Manuel |
author_sort |
Fernández-Lorente, Gloria |
title |
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
title_short |
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
title_full |
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
title_fullStr |
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
title_full_unstemmed |
Modulation of the selectivity of immobilized lipases by chemical and physical modifications: Release of omega-3 fatty acids from fish oil |
title_sort |
modulation of the selectivity of immobilized lipases by chemical and physical modifications: release of omega-3 fatty acids from fish oil |
publisher |
Springer |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/101379 https://doi.org/10.1007/s11746-011-1885-x https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 |
long_lat |
ENVELOPE(-62.233,-62.233,-63.250,-63.250) |
geographic |
Fernandez |
geographic_facet |
Fernandez |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
doi:10.1007/s11746-011-1885-x issn: 0003-021X e-issn: 1558-9331 Journal of the American Oil Chemists' Society 89(1): 97-102 (2012) http://hdl.handle.net/10261/101379 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s11746-011-1885-x10.13039/50110000483710.13039/50110000078010.13039/501100003339 |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
89 |
container_issue |
1 |
container_start_page |
97 |
op_container_end_page |
102 |
_version_ |
1790609608627191808 |