Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization
Abstract Prior studies of the elasmobranch rectal gland have demonstrated that feeding induces profound and rapid up regulation of the gland’s ability to secrete concentrated NaCl solutions and the metabolic capacity to support this highly ATP consuming process. We undertook the current study to att...
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ftcshl:oai:repository.cshl.edu:28651 2023-05-15T18:51:07+02:00 Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization Deck, Courtney A. McKay, Sheldon J. Fiedler, Tristan J. LeMoine, Christophe M. R. Kajimura, Makiko Nawata, C. Michele Wood, Chris M. Walsh, Patrick J. 2013-10-06 http://repository.cshl.edu/id/eprint/28651/ http://www.sciencedirect.com/science/article/pii/S1744117X1300052X https://doi.org/10.1016/j.cbd.2013.09.003 unknown Deck, Courtney A., McKay, Sheldon J., Fiedler, Tristan J., LeMoine, Christophe M. R., Kajimura, Makiko, Nawata, C. Michele, Wood, Chris M., Walsh, Patrick J. (October 2013) Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. ISSN 1744-117X doi:10.1016/j.cbd.2013.09.003 DNA RNA structure function modification genetics & nucleic acid processing genomics and proteomics transcriptomes Paper PeerReviewed 2013 ftcshl https://doi.org/10.1016/j.cbd.2013.09.003 2022-07-17T13:05:52Z Abstract Prior studies of the elasmobranch rectal gland have demonstrated that feeding induces profound and rapid up regulation of the gland’s ability to secrete concentrated NaCl solutions and the metabolic capacity to support this highly ATP consuming process. We undertook the current study to attempt to determine the degree to which up regulation of mRNA transcription was involved in the gland’s activation. cDNA libraries were created from mRNA isolated from rectal glands of fasted (7 days post-feeding) and fed (6 h and 22 h post-feeding) spiny dogfish sharks (Squalus acanthias), and the libraries were subjected to suppression subtractive hybridization (SSH) analysis. Quantitative real time PCR (qPCR) was also used to ascertain the mRNA expression of several genes revealed by the SSH analysis. In total the treatments changed the abundance of 170 transcripts, with 103 up regulated by feeding, and 67 up regulated by fasting. While many of the changes took place in ‘expected’ Gene Ontology (GO) categories (e.g., metabolism, transport, structural proteins, DNA and RNA turnover, etc.), KEGG analysis revealed a number of categories which identify oxidative stress as a topic of interest for the gland. GO analysis also revealed that branched chain essential amino acids (e.g., valine, leucine, isoleucine) as potential metabolic fuels for the rectal gland. In addition, up regulation of transcripts for many genes in the anticipated GO categories did not agree (i.e., fastingdown regulated in feeding treatments) with previously observed increases in their respective proteins/enzyme activities. These results suggest an ‘anticipatory’ storage of selected mRNAs which presumably supports the rapid translation of proteins upon feeding activation of the gland. Report spiny dogfish Squalus acanthias Cold Spring Harbor Laboratory: CSHL Institutional Repository Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 8 4 334 343 |
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collection |
Cold Spring Harbor Laboratory: CSHL Institutional Repository |
op_collection_id |
ftcshl |
language |
unknown |
topic |
DNA RNA structure function modification genetics & nucleic acid processing genomics and proteomics transcriptomes |
spellingShingle |
DNA RNA structure function modification genetics & nucleic acid processing genomics and proteomics transcriptomes Deck, Courtney A. McKay, Sheldon J. Fiedler, Tristan J. LeMoine, Christophe M. R. Kajimura, Makiko Nawata, C. Michele Wood, Chris M. Walsh, Patrick J. Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
topic_facet |
DNA RNA structure function modification genetics & nucleic acid processing genomics and proteomics transcriptomes |
description |
Abstract Prior studies of the elasmobranch rectal gland have demonstrated that feeding induces profound and rapid up regulation of the gland’s ability to secrete concentrated NaCl solutions and the metabolic capacity to support this highly ATP consuming process. We undertook the current study to attempt to determine the degree to which up regulation of mRNA transcription was involved in the gland’s activation. cDNA libraries were created from mRNA isolated from rectal glands of fasted (7 days post-feeding) and fed (6 h and 22 h post-feeding) spiny dogfish sharks (Squalus acanthias), and the libraries were subjected to suppression subtractive hybridization (SSH) analysis. Quantitative real time PCR (qPCR) was also used to ascertain the mRNA expression of several genes revealed by the SSH analysis. In total the treatments changed the abundance of 170 transcripts, with 103 up regulated by feeding, and 67 up regulated by fasting. While many of the changes took place in ‘expected’ Gene Ontology (GO) categories (e.g., metabolism, transport, structural proteins, DNA and RNA turnover, etc.), KEGG analysis revealed a number of categories which identify oxidative stress as a topic of interest for the gland. GO analysis also revealed that branched chain essential amino acids (e.g., valine, leucine, isoleucine) as potential metabolic fuels for the rectal gland. In addition, up regulation of transcripts for many genes in the anticipated GO categories did not agree (i.e., fastingdown regulated in feeding treatments) with previously observed increases in their respective proteins/enzyme activities. These results suggest an ‘anticipatory’ storage of selected mRNAs which presumably supports the rapid translation of proteins upon feeding activation of the gland. |
format |
Report |
author |
Deck, Courtney A. McKay, Sheldon J. Fiedler, Tristan J. LeMoine, Christophe M. R. Kajimura, Makiko Nawata, C. Michele Wood, Chris M. Walsh, Patrick J. |
author_facet |
Deck, Courtney A. McKay, Sheldon J. Fiedler, Tristan J. LeMoine, Christophe M. R. Kajimura, Makiko Nawata, C. Michele Wood, Chris M. Walsh, Patrick J. |
author_sort |
Deck, Courtney A. |
title |
Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
title_short |
Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
title_full |
Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
title_fullStr |
Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
title_full_unstemmed |
Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization |
title_sort |
transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (squalus acanthias) determined by suppression subtractive hybridization |
publishDate |
2013 |
url |
http://repository.cshl.edu/id/eprint/28651/ http://www.sciencedirect.com/science/article/pii/S1744117X1300052X https://doi.org/10.1016/j.cbd.2013.09.003 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
Deck, Courtney A., McKay, Sheldon J., Fiedler, Tristan J., LeMoine, Christophe M. R., Kajimura, Makiko, Nawata, C. Michele, Wood, Chris M., Walsh, Patrick J. (October 2013) Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. ISSN 1744-117X doi:10.1016/j.cbd.2013.09.003 |
op_doi |
https://doi.org/10.1016/j.cbd.2013.09.003 |
container_title |
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
container_volume |
8 |
container_issue |
4 |
container_start_page |
334 |
op_container_end_page |
343 |
_version_ |
1766244905936486400 |