Carbonic anhydrase is involved in benthic foraminiferal calcification
Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium...
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ftcopernicus:oai:publications.copernicus.org:bgd79908 2023-05-15T17:51:17+02:00 Carbonic anhydrase is involved in benthic foraminiferal calcification Goeyse, Siham Webb, Alice E. Reichart, Gert-Jan Nooijer, Lennart J. 2019-09-23 application/pdf https://doi.org/10.5194/bg-2019-356 https://www.biogeosciences-discuss.net/bg-2019-356/ eng eng doi:10.5194/bg-2019-356 https://www.biogeosciences-discuss.net/bg-2019-356/ eISSN: 1726-4189 Text 2019 ftcopernicus https://doi.org/10.5194/bg-2019-356 2019-12-24T09:48:29Z Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium carbonate production, the process by which they concentrate inorganic carbon is debated. Some evidence suggests that seawater is taken up and participates relatively unaltered in the process of calcification, whereas other results suggest the involvement of transmembrane transport and the activity of enzymes like carbonic anhydrase. Here, we tested whether inorganic carbon uptake relies on the activity of carbonic anhydrase using incubation experiments with the large benthic, symbiont-bearing foraminifer Amphistegina lessonii . Calcification rates, determined by the alkalinity anomaly method, showed that inhibition of carbonic anhydrase by acetazolamide (AZ) stopped most of the calcification process. Inhibition of photosynthesis by either 3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU) or by incubating the foraminifera in the dark, also decreased calcification rates, but to a lesser degree than with AZ. Results from this study show that carbonic anhydrase plays a key role in biomineralization of Amphistegina lessonii and indicates that calcification of those large benthic foraminifera might, to a certain extent, benefit from ocean acidification. Text Ocean acidification Copernicus Publications: E-Journals |
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English |
description |
Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium carbonate production, the process by which they concentrate inorganic carbon is debated. Some evidence suggests that seawater is taken up and participates relatively unaltered in the process of calcification, whereas other results suggest the involvement of transmembrane transport and the activity of enzymes like carbonic anhydrase. Here, we tested whether inorganic carbon uptake relies on the activity of carbonic anhydrase using incubation experiments with the large benthic, symbiont-bearing foraminifer Amphistegina lessonii . Calcification rates, determined by the alkalinity anomaly method, showed that inhibition of carbonic anhydrase by acetazolamide (AZ) stopped most of the calcification process. Inhibition of photosynthesis by either 3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU) or by incubating the foraminifera in the dark, also decreased calcification rates, but to a lesser degree than with AZ. Results from this study show that carbonic anhydrase plays a key role in biomineralization of Amphistegina lessonii and indicates that calcification of those large benthic foraminifera might, to a certain extent, benefit from ocean acidification. |
format |
Text |
author |
Goeyse, Siham Webb, Alice E. Reichart, Gert-Jan Nooijer, Lennart J. |
spellingShingle |
Goeyse, Siham Webb, Alice E. Reichart, Gert-Jan Nooijer, Lennart J. Carbonic anhydrase is involved in benthic foraminiferal calcification |
author_facet |
Goeyse, Siham Webb, Alice E. Reichart, Gert-Jan Nooijer, Lennart J. |
author_sort |
Goeyse, Siham |
title |
Carbonic anhydrase is involved in benthic foraminiferal calcification |
title_short |
Carbonic anhydrase is involved in benthic foraminiferal calcification |
title_full |
Carbonic anhydrase is involved in benthic foraminiferal calcification |
title_fullStr |
Carbonic anhydrase is involved in benthic foraminiferal calcification |
title_full_unstemmed |
Carbonic anhydrase is involved in benthic foraminiferal calcification |
title_sort |
carbonic anhydrase is involved in benthic foraminiferal calcification |
publishDate |
2019 |
url |
https://doi.org/10.5194/bg-2019-356 https://www.biogeosciences-discuss.net/bg-2019-356/ |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
eISSN: 1726-4189 |
op_relation |
doi:10.5194/bg-2019-356 https://www.biogeosciences-discuss.net/bg-2019-356/ |
op_doi |
https://doi.org/10.5194/bg-2019-356 |
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1766158389762588672 |