Effects of atmospheric conditions on ice nucleation activity of Pseudomonas
Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO 2 and O 3 exposure...
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ftcopernicus:oai:publications.copernicus.org:acp14852 2023-05-15T13:45:55+02:00 Effects of atmospheric conditions on ice nucleation activity of Pseudomonas Attard, E. Yang, H. Delort, A.-M. Amato, P. Pöschl, U. Glaux, C. Koop, T. Morris, C. E. 2018-01-15 application/pdf https://doi.org/10.5194/acp-12-10667-2012 https://www.atmos-chem-phys.net/12/10667/2012/ eng eng doi:10.5194/acp-12-10667-2012 https://www.atmos-chem-phys.net/12/10667/2012/ eISSN: 1680-7324 Text 2018 ftcopernicus https://doi.org/10.5194/acp-12-10667-2012 2019-12-24T09:55:43Z Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO 2 and O 3 exposure and (iii) UV-A exposure on the ice nucleation activity (INA) of four Pseudomonas strains. Three of the Pseudomonas syringae strains were isolated from cloud water and the phyllosphere and Pseudomonas fluorescens strain CGina-01 was isolated from Antarctic glacier ice melt. Among the three conditions tested, acidic pH caused the most significant effects on INA likely due to denaturation of the ice nucleation protein complex. Exposure to NO 2 and O 3 gases had no significant or only weak effects on the INA of two P. syringae strains whereas the INA of P. fluorescens CGina-01 was significantly affected. The INA of the third P. syringae strain showed variable responses to NO 2 and O 3 exposure. These differences in the INA of different Pseudomonas suggest that the response to atmospheric conditions could be strain-specific. After UV-A exposure, a substantial loss of viability of all four strains was observed whereas their INA decreased only slightly. This corroborates the notion that under certain conditions dead bacterial cells can maintain their INA. Overall, the negative effects of the three environmental factors on INA were more significant at the warmer temperatures. Our results suggest that in clouds where temperatures are near 0 °C, the importance of bacterial ice nucleation in precipitation processes could be reduced by some environmental factors. Text Antarc* Antarctic Copernicus Publications: E-Journals Antarctic Atmospheric Chemistry and Physics 12 22 10667 10677 |
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Copernicus Publications: E-Journals |
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ftcopernicus |
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English |
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Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO 2 and O 3 exposure and (iii) UV-A exposure on the ice nucleation activity (INA) of four Pseudomonas strains. Three of the Pseudomonas syringae strains were isolated from cloud water and the phyllosphere and Pseudomonas fluorescens strain CGina-01 was isolated from Antarctic glacier ice melt. Among the three conditions tested, acidic pH caused the most significant effects on INA likely due to denaturation of the ice nucleation protein complex. Exposure to NO 2 and O 3 gases had no significant or only weak effects on the INA of two P. syringae strains whereas the INA of P. fluorescens CGina-01 was significantly affected. The INA of the third P. syringae strain showed variable responses to NO 2 and O 3 exposure. These differences in the INA of different Pseudomonas suggest that the response to atmospheric conditions could be strain-specific. After UV-A exposure, a substantial loss of viability of all four strains was observed whereas their INA decreased only slightly. This corroborates the notion that under certain conditions dead bacterial cells can maintain their INA. Overall, the negative effects of the three environmental factors on INA were more significant at the warmer temperatures. Our results suggest that in clouds where temperatures are near 0 °C, the importance of bacterial ice nucleation in precipitation processes could be reduced by some environmental factors. |
format |
Text |
author |
Attard, E. Yang, H. Delort, A.-M. Amato, P. Pöschl, U. Glaux, C. Koop, T. Morris, C. E. |
spellingShingle |
Attard, E. Yang, H. Delort, A.-M. Amato, P. Pöschl, U. Glaux, C. Koop, T. Morris, C. E. Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
author_facet |
Attard, E. Yang, H. Delort, A.-M. Amato, P. Pöschl, U. Glaux, C. Koop, T. Morris, C. E. |
author_sort |
Attard, E. |
title |
Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
title_short |
Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
title_full |
Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
title_fullStr |
Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
title_full_unstemmed |
Effects of atmospheric conditions on ice nucleation activity of Pseudomonas |
title_sort |
effects of atmospheric conditions on ice nucleation activity of pseudomonas |
publishDate |
2018 |
url |
https://doi.org/10.5194/acp-12-10667-2012 https://www.atmos-chem-phys.net/12/10667/2012/ |
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Antarctic |
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Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
eISSN: 1680-7324 |
op_relation |
doi:10.5194/acp-12-10667-2012 https://www.atmos-chem-phys.net/12/10667/2012/ |
op_doi |
https://doi.org/10.5194/acp-12-10667-2012 |
container_title |
Atmospheric Chemistry and Physics |
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12 |
container_issue |
22 |
container_start_page |
10667 |
op_container_end_page |
10677 |
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1766232457117433856 |