High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout
The Gram-negative bacterium A. salmonicida is the causal agent of furunculosis and used to be one of the most loss-causing bacterial infections in the salmonid aquaculture industry with a mortality rate of about 90% until the 1990s, when an inactivated vaccine with mineral oil as adjuvant was succes...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2023
|
Subjects: | |
Online Access: | https://curis.ku.dk/portal/da/publications/high-immunogenicity-of-viruslike-particles-vlps-decorated-with-aeromonas-salmonicida-vapa-antigen-in-rainbow-trout(f0c6f8d9-f002-4139-b14c-3f51d288426a).html https://doi.org/10.3389/fimmu.2023.1139206 https://curis.ku.dk/ws/files/357056181/fimmu_14_1139206.pdf http://www.scopus.com/inward/record.url?scp=85161088509&partnerID=8YFLogxK |
id |
ftcopenhagenunip:oai:pure.atira.dk:publications/f0c6f8d9-f002-4139-b14c-3f51d288426a |
---|---|
record_format |
openpolar |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/f0c6f8d9-f002-4139-b14c-3f51d288426a 2024-05-19T07:37:49+00:00 High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout Yang, Jeong In Sepúlveda, Dagoberto Vardia, Irina Skov, Jakob Goksøyr, Louise Sander, Adam F. Lorenzen, Niels 2023 application/pdf https://curis.ku.dk/portal/da/publications/high-immunogenicity-of-viruslike-particles-vlps-decorated-with-aeromonas-salmonicida-vapa-antigen-in-rainbow-trout(f0c6f8d9-f002-4139-b14c-3f51d288426a).html https://doi.org/10.3389/fimmu.2023.1139206 https://curis.ku.dk/ws/files/357056181/fimmu_14_1139206.pdf http://www.scopus.com/inward/record.url?scp=85161088509&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Yang , J I , Sepúlveda , D , Vardia , I , Skov , J , Goksøyr , L , Sander , A F & Lorenzen , N 2023 , ' High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout ' , Frontiers in Immunology , vol. 14 , 1139206 . https://doi.org/10.3389/fimmu.2023.1139206 A. salmonicida aquaculture fish diseases furunculosis recombinant vaccine Virus-like particles (VLPs) article 2023 ftcopenhagenunip https://doi.org/10.3389/fimmu.2023.1139206 2024-05-02T00:33:21Z The Gram-negative bacterium A. salmonicida is the causal agent of furunculosis and used to be one of the most loss-causing bacterial infections in the salmonid aquaculture industry with a mortality rate of about 90% until the 1990s, when an inactivated vaccine with mineral oil as adjuvant was successfully implemented to control the disease. However, the use of this vaccine is associated with inflammatory side effects in the peritoneal cavity as well as autoimmune reactions in Atlantic salmon, and incomplete protection has been reported in rainbow trout. We here aimed at developing and testing a recombinant alternative vaccine based on virus-like particles (VLPs) decorated with VapA, the key structural surface protein in the outer A-layer of A. salmonicida. The VLP carrier was based on either the capsid protein of a fish nodavirus, namely red grouper nervous necrotic virus (RGNNV) or the capsid protein of Acinetobacter phage AP205. The VapA and capsid proteins were expressed individually in E. coli and VapA was fused to auto-assembled VLPs using the SpyTag/SpyCatcher technology. Rainbow trout were vaccinated/immunized with the VapA-VLP vaccines by intraperitoneal injection and were challenged with A. salmonicida 7 weeks later. The VLP vaccines provided protection comparable to that of a bacterin-based vaccine and antibody response analysis demonstrated that vaccinated fish mounted a strong VapA-specific antibody response. To our knowledge, this is the first demonstration of the potential use of antigen-decorated VLPs for vaccination against a bacterial disease in salmonids. Article in Journal/Newspaper Atlantic salmon University of Copenhagen: Research Frontiers in Immunology 14 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
A. salmonicida aquaculture fish diseases furunculosis recombinant vaccine Virus-like particles (VLPs) |
spellingShingle |
A. salmonicida aquaculture fish diseases furunculosis recombinant vaccine Virus-like particles (VLPs) Yang, Jeong In Sepúlveda, Dagoberto Vardia, Irina Skov, Jakob Goksøyr, Louise Sander, Adam F. Lorenzen, Niels High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
topic_facet |
A. salmonicida aquaculture fish diseases furunculosis recombinant vaccine Virus-like particles (VLPs) |
description |
The Gram-negative bacterium A. salmonicida is the causal agent of furunculosis and used to be one of the most loss-causing bacterial infections in the salmonid aquaculture industry with a mortality rate of about 90% until the 1990s, when an inactivated vaccine with mineral oil as adjuvant was successfully implemented to control the disease. However, the use of this vaccine is associated with inflammatory side effects in the peritoneal cavity as well as autoimmune reactions in Atlantic salmon, and incomplete protection has been reported in rainbow trout. We here aimed at developing and testing a recombinant alternative vaccine based on virus-like particles (VLPs) decorated with VapA, the key structural surface protein in the outer A-layer of A. salmonicida. The VLP carrier was based on either the capsid protein of a fish nodavirus, namely red grouper nervous necrotic virus (RGNNV) or the capsid protein of Acinetobacter phage AP205. The VapA and capsid proteins were expressed individually in E. coli and VapA was fused to auto-assembled VLPs using the SpyTag/SpyCatcher technology. Rainbow trout were vaccinated/immunized with the VapA-VLP vaccines by intraperitoneal injection and were challenged with A. salmonicida 7 weeks later. The VLP vaccines provided protection comparable to that of a bacterin-based vaccine and antibody response analysis demonstrated that vaccinated fish mounted a strong VapA-specific antibody response. To our knowledge, this is the first demonstration of the potential use of antigen-decorated VLPs for vaccination against a bacterial disease in salmonids. |
format |
Article in Journal/Newspaper |
author |
Yang, Jeong In Sepúlveda, Dagoberto Vardia, Irina Skov, Jakob Goksøyr, Louise Sander, Adam F. Lorenzen, Niels |
author_facet |
Yang, Jeong In Sepúlveda, Dagoberto Vardia, Irina Skov, Jakob Goksøyr, Louise Sander, Adam F. Lorenzen, Niels |
author_sort |
Yang, Jeong In |
title |
High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
title_short |
High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
title_full |
High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
title_fullStr |
High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
title_full_unstemmed |
High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout |
title_sort |
high immunogenicity of virus-like particles (vlps) decorated with aeromonas salmonicida vapa antigen in rainbow trout |
publishDate |
2023 |
url |
https://curis.ku.dk/portal/da/publications/high-immunogenicity-of-viruslike-particles-vlps-decorated-with-aeromonas-salmonicida-vapa-antigen-in-rainbow-trout(f0c6f8d9-f002-4139-b14c-3f51d288426a).html https://doi.org/10.3389/fimmu.2023.1139206 https://curis.ku.dk/ws/files/357056181/fimmu_14_1139206.pdf http://www.scopus.com/inward/record.url?scp=85161088509&partnerID=8YFLogxK |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Yang , J I , Sepúlveda , D , Vardia , I , Skov , J , Goksøyr , L , Sander , A F & Lorenzen , N 2023 , ' High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout ' , Frontiers in Immunology , vol. 14 , 1139206 . https://doi.org/10.3389/fimmu.2023.1139206 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fimmu.2023.1139206 |
container_title |
Frontiers in Immunology |
container_volume |
14 |
_version_ |
1799477198376140800 |