Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients
The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery...
Published in: | European Archives of Oto-Rhino-Laryngology |
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2015
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ftcopenhagenunip:oai:pure.atira.dk:publications/dfd978a6-800a-4a59-806d-b5efaa0ade9e 2023-05-15T16:31:08+02:00 Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients Wessman, Marcus Bjarnsholt, Thomas Eickhardt-Sørensen, Steffen Robert Johansen, Helle Krogh Homøe, Preben 2015-05 https://curis.ku.dk/portal/da/publications/mucosal-biofilm-detection-in-chronic-otitis-media(dfd978a6-800a-4a59-806d-b5efaa0ade9e).html https://doi.org/10.1007/s00405-014-2886-9 eng eng info:eu-repo/semantics/closedAccess Wessman , M , Bjarnsholt , T , Eickhardt-Sørensen , S R , Johansen , H K & Homøe , P 2015 , ' Mucosal biofilm detection in chronic otitis media : a study of middle ear biopsies from Greenlandic patients ' , European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery , vol. 272 , no. 5 , pp. 1079-1085 . https://doi.org/10.1007/s00405-014-2886-9 article 2015 ftcopenhagenunip https://doi.org/10.1007/s00405-014-2886-9 2021-10-06T22:56:38Z The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary. Article in Journal/Newspaper greenlandic University of Copenhagen: Research European Archives of Oto-Rhino-Laryngology 272 5 1079 1085 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
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ftcopenhagenunip |
language |
English |
description |
The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary. |
format |
Article in Journal/Newspaper |
author |
Wessman, Marcus Bjarnsholt, Thomas Eickhardt-Sørensen, Steffen Robert Johansen, Helle Krogh Homøe, Preben |
spellingShingle |
Wessman, Marcus Bjarnsholt, Thomas Eickhardt-Sørensen, Steffen Robert Johansen, Helle Krogh Homøe, Preben Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
author_facet |
Wessman, Marcus Bjarnsholt, Thomas Eickhardt-Sørensen, Steffen Robert Johansen, Helle Krogh Homøe, Preben |
author_sort |
Wessman, Marcus |
title |
Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
title_short |
Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
title_full |
Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
title_fullStr |
Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
title_full_unstemmed |
Mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from Greenlandic patients |
title_sort |
mucosal biofilm detection in chronic otitis media:a study of middle ear biopsies from greenlandic patients |
publishDate |
2015 |
url |
https://curis.ku.dk/portal/da/publications/mucosal-biofilm-detection-in-chronic-otitis-media(dfd978a6-800a-4a59-806d-b5efaa0ade9e).html https://doi.org/10.1007/s00405-014-2886-9 |
genre |
greenlandic |
genre_facet |
greenlandic |
op_source |
Wessman , M , Bjarnsholt , T , Eickhardt-Sørensen , S R , Johansen , H K & Homøe , P 2015 , ' Mucosal biofilm detection in chronic otitis media : a study of middle ear biopsies from Greenlandic patients ' , European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery , vol. 272 , no. 5 , pp. 1079-1085 . https://doi.org/10.1007/s00405-014-2886-9 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00405-014-2886-9 |
container_title |
European Archives of Oto-Rhino-Laryngology |
container_volume |
272 |
container_issue |
5 |
container_start_page |
1079 |
op_container_end_page |
1085 |
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