Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel
Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively lim...
Published in: | Parasitology Research |
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Language: | English |
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2021
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Online Access: | https://curis.ku.dk/portal/da/publications/morphological-and-molecular-identification-of-cryptocotyle-lingua-metacercariae-isolated-from-atlantic-cod-gadus-morhua-from-danish-seas-and-whiting-merlangius-merlangus-from-the-english-channel(d895646d-c895-4101-ac5e-920326eccc52).html https://doi.org/10.1007/s00436-021-07278-6 https://curis.ku.dk/ws/files/281101231/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf |
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ftcopenhagenunip:oai:pure.atira.dk:publications/d895646d-c895-4101-ac5e-920326eccc52 2024-06-09T07:44:37+00:00 Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt 2021 application/pdf https://curis.ku.dk/portal/da/publications/morphological-and-molecular-identification-of-cryptocotyle-lingua-metacercariae-isolated-from-atlantic-cod-gadus-morhua-from-danish-seas-and-whiting-merlangius-merlangus-from-the-english-channel(d895646d-c895-4101-ac5e-920326eccc52).html https://doi.org/10.1007/s00436-021-07278-6 https://curis.ku.dk/ws/files/281101231/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf eng eng info:eu-repo/semantics/openAccess Duflot , M , Gay , M , Midelet , G , Kania , P W & Buchmann , K 2021 , ' Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel ' , Parasitology Research , vol. 120 , pp. 3417–3427 . https://doi.org/10.1007/s00436-021-07278-6 Cryptocotyle English Channel Heterophyidae Molecular identification Morphological identification Whiting article 2021 ftcopenhagenunip https://doi.org/10.1007/s00436-021-07278-6 2024-05-16T11:29:21Z Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting (Merlangius merlangus) and Atlantic cod (Gadus morhua) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. Article in Journal/Newspaper atlantic cod Gadus morhua University of Copenhagen: Research Parasitology Research 120 10 3417 3427 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Cryptocotyle English Channel Heterophyidae Molecular identification Morphological identification Whiting |
spellingShingle |
Cryptocotyle English Channel Heterophyidae Molecular identification Morphological identification Whiting Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
topic_facet |
Cryptocotyle English Channel Heterophyidae Molecular identification Morphological identification Whiting |
description |
Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting (Merlangius merlangus) and Atlantic cod (Gadus morhua) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. |
format |
Article in Journal/Newspaper |
author |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_facet |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_sort |
Duflot, Maureen |
title |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
title_short |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
title_full |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
title_fullStr |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
title_full_unstemmed |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel |
title_sort |
morphological and molecular identification of cryptocotyle lingua metacercariae isolated from atlantic cod ( gadus morhua ) from danish seas and whiting ( merlangius merlangus ) from the english channel |
publishDate |
2021 |
url |
https://curis.ku.dk/portal/da/publications/morphological-and-molecular-identification-of-cryptocotyle-lingua-metacercariae-isolated-from-atlantic-cod-gadus-morhua-from-danish-seas-and-whiting-merlangius-merlangus-from-the-english-channel(d895646d-c895-4101-ac5e-920326eccc52).html https://doi.org/10.1007/s00436-021-07278-6 https://curis.ku.dk/ws/files/281101231/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Duflot , M , Gay , M , Midelet , G , Kania , P W & Buchmann , K 2021 , ' Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod ( Gadus morhua ) from Danish seas and whiting ( Merlangius merlangus ) from the English Channel ' , Parasitology Research , vol. 120 , pp. 3417–3427 . https://doi.org/10.1007/s00436-021-07278-6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00436-021-07278-6 |
container_title |
Parasitology Research |
container_volume |
120 |
container_issue |
10 |
container_start_page |
3417 |
op_container_end_page |
3427 |
_version_ |
1801373417063055360 |