Supraglacial bacterial community structures vary across the Greenland ice sheet
The composition and spatial variability of microbial communities that reside within the extensive (>200 000 km(2)) biologically active area encompassing the Greenland ice sheet (GrIS) is hypothesized to be variable. We examined bacterial communities from cryoconite debris and surface ice across t...
Published in: | FEMS Microbiology Ecology |
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Language: | English |
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2016
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Online Access: | https://curis.ku.dk/portal/da/publications/supraglacial-bacterial-community-structures-vary-across-the-greenland-ice-sheet(d6f55e79-e0f9-4b92-aa1c-033bdd9012e2).html https://doi.org/10.1093/femsec/fiv164 |
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ftcopenhagenunip:oai:pure.atira.dk:publications/d6f55e79-e0f9-4b92-aa1c-033bdd9012e2 2023-05-15T16:27:51+02:00 Supraglacial bacterial community structures vary across the Greenland ice sheet Cameron, Karen A. Stibal, Marek Zarsky, Jakub D. Gözdereliler, Erkin Schostag, Morten Dencker Jacobsen, Carsten Suhr 2016 https://curis.ku.dk/portal/da/publications/supraglacial-bacterial-community-structures-vary-across-the-greenland-ice-sheet(d6f55e79-e0f9-4b92-aa1c-033bdd9012e2).html https://doi.org/10.1093/femsec/fiv164 eng eng info:eu-repo/semantics/closedAccess Cameron , K A , Stibal , M , Zarsky , J D , Gözdereliler , E , Schostag , M D & Jacobsen , C S 2016 , ' Supraglacial bacterial community structures vary across the Greenland ice sheet ' , F E M S Microbiology Ecology , vol. 92 , no. 2 . https://doi.org/10.1093/femsec/fiv164 article 2016 ftcopenhagenunip https://doi.org/10.1093/femsec/fiv164 2022-02-24T00:20:29Z The composition and spatial variability of microbial communities that reside within the extensive (>200 000 km(2)) biologically active area encompassing the Greenland ice sheet (GrIS) is hypothesized to be variable. We examined bacterial communities from cryoconite debris and surface ice across the GrIS, using sequence analysis and quantitative PCR of 16S rRNA genes from co-extracted DNA and RNA. Communities were found to differ across the ice sheet, with 82.8% of the total calculated variation attributed to spatial distribution on a scale of tens of kilometers separation. Amplicons related to Sphingobacteriaceae, Pseudanabaenaceae and WPS-2 accounted for the greatest portion of calculated dissimilarities. The bacterial communities of ice and cryoconite were moderately similar (global R = 0.360, P = 0.002) and the sampled surface type (ice versus cryoconite) did not contribute heavily towards community dissimilarities (2.3% of total variability calculated). The majority of dissimilarities found between cryoconite 16S rRNA gene amplicons from DNA and RNA was calculated to be the result of changes in three taxa, Pseudanabaenaceae, Sphingobacteriaceae and WPS-2, which together contributed towards 80.8 ± 12.6% of dissimilarities between samples. Bacterial communities across the GrIS are spatially variable active communities that are likely influenced by localized biological inputs and physicochemical conditions. Article in Journal/Newspaper Greenland Ice Sheet University of Copenhagen: Research Greenland FEMS Microbiology Ecology 92 2 fiv164 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
description |
The composition and spatial variability of microbial communities that reside within the extensive (>200 000 km(2)) biologically active area encompassing the Greenland ice sheet (GrIS) is hypothesized to be variable. We examined bacterial communities from cryoconite debris and surface ice across the GrIS, using sequence analysis and quantitative PCR of 16S rRNA genes from co-extracted DNA and RNA. Communities were found to differ across the ice sheet, with 82.8% of the total calculated variation attributed to spatial distribution on a scale of tens of kilometers separation. Amplicons related to Sphingobacteriaceae, Pseudanabaenaceae and WPS-2 accounted for the greatest portion of calculated dissimilarities. The bacterial communities of ice and cryoconite were moderately similar (global R = 0.360, P = 0.002) and the sampled surface type (ice versus cryoconite) did not contribute heavily towards community dissimilarities (2.3% of total variability calculated). The majority of dissimilarities found between cryoconite 16S rRNA gene amplicons from DNA and RNA was calculated to be the result of changes in three taxa, Pseudanabaenaceae, Sphingobacteriaceae and WPS-2, which together contributed towards 80.8 ± 12.6% of dissimilarities between samples. Bacterial communities across the GrIS are spatially variable active communities that are likely influenced by localized biological inputs and physicochemical conditions. |
format |
Article in Journal/Newspaper |
author |
Cameron, Karen A. Stibal, Marek Zarsky, Jakub D. Gözdereliler, Erkin Schostag, Morten Dencker Jacobsen, Carsten Suhr |
spellingShingle |
Cameron, Karen A. Stibal, Marek Zarsky, Jakub D. Gözdereliler, Erkin Schostag, Morten Dencker Jacobsen, Carsten Suhr Supraglacial bacterial community structures vary across the Greenland ice sheet |
author_facet |
Cameron, Karen A. Stibal, Marek Zarsky, Jakub D. Gözdereliler, Erkin Schostag, Morten Dencker Jacobsen, Carsten Suhr |
author_sort |
Cameron, Karen A. |
title |
Supraglacial bacterial community structures vary across the Greenland ice sheet |
title_short |
Supraglacial bacterial community structures vary across the Greenland ice sheet |
title_full |
Supraglacial bacterial community structures vary across the Greenland ice sheet |
title_fullStr |
Supraglacial bacterial community structures vary across the Greenland ice sheet |
title_full_unstemmed |
Supraglacial bacterial community structures vary across the Greenland ice sheet |
title_sort |
supraglacial bacterial community structures vary across the greenland ice sheet |
publishDate |
2016 |
url |
https://curis.ku.dk/portal/da/publications/supraglacial-bacterial-community-structures-vary-across-the-greenland-ice-sheet(d6f55e79-e0f9-4b92-aa1c-033bdd9012e2).html https://doi.org/10.1093/femsec/fiv164 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
Cameron , K A , Stibal , M , Zarsky , J D , Gözdereliler , E , Schostag , M D & Jacobsen , C S 2016 , ' Supraglacial bacterial community structures vary across the Greenland ice sheet ' , F E M S Microbiology Ecology , vol. 92 , no. 2 . https://doi.org/10.1093/femsec/fiv164 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1093/femsec/fiv164 |
container_title |
FEMS Microbiology Ecology |
container_volume |
92 |
container_issue |
2 |
container_start_page |
fiv164 |
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1766017408463536128 |