Constructing and screening a metagenomic library of a cold and alkaline extreme environment
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a sou...
Main Authors: | , , |
---|---|
Other Authors: | , |
Format: | Book Part |
Language: | English |
Published: |
Springer
2017
|
Subjects: | |
Online Access: | https://curis.ku.dk/portal/da/publications/constructing-and-screening-a-metagenomic-library-of-a-cold-and-alkaline-extreme-environment(d6a7234d-7c0d-411c-9b22-56a7244d9421).html https://doi.org/10.1007/978-1-4939-6691-2_4 |
id |
ftcopenhagenunip:oai:pure.atira.dk:publications/d6a7234d-7c0d-411c-9b22-56a7244d9421 |
---|---|
record_format |
openpolar |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/d6a7234d-7c0d-411c-9b22-56a7244d9421 2023-07-16T03:58:45+02:00 Constructing and screening a metagenomic library of a cold and alkaline extreme environment Glaring, Mikkel Andreas Vester, Jan Kjølhede Stougaard, Peter Streit, Wolfgang R. Daniel, Rolf 2017 https://curis.ku.dk/portal/da/publications/constructing-and-screening-a-metagenomic-library-of-a-cold-and-alkaline-extreme-environment(d6a7234d-7c0d-411c-9b22-56a7244d9421).html https://doi.org/10.1007/978-1-4939-6691-2_4 eng eng Springer info:eu-repo/semantics/closedAccess Glaring , M A , Vester , J K & Stougaard , P 2017 , Constructing and screening a metagenomic library of a cold and alkaline extreme environment . in W R Streit & R Daniel (eds) , Metagenomics . Springer , Methods in Molecular Biology , vol. 1539 , pp. 43-55 . https://doi.org/10.1007/978-1-4939-6691-2_4 Journal Article bookPart 2017 ftcopenhagenunip https://doi.org/10.1007/978-1-4939-6691-2_4 2023-06-28T23:01:34Z Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns. Book Part Greenland University of Copenhagen: Research Greenland Ikka ENVELOPE(-48.100,-48.100,61.150,61.150) 43 55 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Journal Article |
spellingShingle |
Journal Article Glaring, Mikkel Andreas Vester, Jan Kjølhede Stougaard, Peter Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
topic_facet |
Journal Article |
description |
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns. |
author2 |
Streit, Wolfgang R. Daniel, Rolf |
format |
Book Part |
author |
Glaring, Mikkel Andreas Vester, Jan Kjølhede Stougaard, Peter |
author_facet |
Glaring, Mikkel Andreas Vester, Jan Kjølhede Stougaard, Peter |
author_sort |
Glaring, Mikkel Andreas |
title |
Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
title_short |
Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
title_full |
Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
title_fullStr |
Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
title_full_unstemmed |
Constructing and screening a metagenomic library of a cold and alkaline extreme environment |
title_sort |
constructing and screening a metagenomic library of a cold and alkaline extreme environment |
publisher |
Springer |
publishDate |
2017 |
url |
https://curis.ku.dk/portal/da/publications/constructing-and-screening-a-metagenomic-library-of-a-cold-and-alkaline-extreme-environment(d6a7234d-7c0d-411c-9b22-56a7244d9421).html https://doi.org/10.1007/978-1-4939-6691-2_4 |
long_lat |
ENVELOPE(-48.100,-48.100,61.150,61.150) |
geographic |
Greenland Ikka |
geographic_facet |
Greenland Ikka |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Glaring , M A , Vester , J K & Stougaard , P 2017 , Constructing and screening a metagenomic library of a cold and alkaline extreme environment . in W R Streit & R Daniel (eds) , Metagenomics . Springer , Methods in Molecular Biology , vol. 1539 , pp. 43-55 . https://doi.org/10.1007/978-1-4939-6691-2_4 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/978-1-4939-6691-2_4 |
container_start_page |
43 |
op_container_end_page |
55 |
_version_ |
1771546038300049408 |