Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios?
Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spi...
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Language: | English |
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2021
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Online Access: | https://curis.ku.dk/portal/da/publications/induction-of-conjugation-and-zygospore-cell-wall-characteristics-in-the-alpine-spirogyra-mirabilis-zygnematophyceae-charophyta(ce4e3d24-5e7f-427c-8bac-785f649418d6).html https://doi.org/10.3390/plants10081740 https://curis.ku.dk/ws/files/279256591/plants_10_01740_v2.pdf |
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ftcopenhagenunip:oai:pure.atira.dk:publications/ce4e3d24-5e7f-427c-8bac-785f649418d6 2024-06-09T07:38:43+00:00 Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? Permann, Charlotte Herburger, Klaus Felhofer, Martin Gierlinger, Notburga Lewis, Louise A. Holzinger, Andreas 2021 application/pdf https://curis.ku.dk/portal/da/publications/induction-of-conjugation-and-zygospore-cell-wall-characteristics-in-the-alpine-spirogyra-mirabilis-zygnematophyceae-charophyta(ce4e3d24-5e7f-427c-8bac-785f649418d6).html https://doi.org/10.3390/plants10081740 https://curis.ku.dk/ws/files/279256591/plants_10_01740_v2.pdf eng eng info:eu-repo/semantics/openAccess Permann , C , Herburger , K , Felhofer , M , Gierlinger , N , Lewis , L A & Holzinger , A 2021 , ' Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta) : Advantage under Climate Change Scenarios? ' , Plants , vol. 10 , no. 8 , 1740 . https://doi.org/10.3390/plants10081740 alpine region cell wall conjugation Spirogyra sexual reproduction streptophyte zygospore GREEN-ALGA ZYGNEMA FRESH-WATER TERRESTRIAL ALGAE ANTARCTIC STRAINS NY-ALESUND STREPTOPHYTA DIVERSITY EVOLUTION PHOTOSYNTHESIS article 2021 ftcopenhagenunip https://doi.org/10.3390/plants10081740 2024-05-16T11:29:21Z Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spirogyra with numerous conjugating stages was isolated and characterized by molecular phylogeny. We successfully induced sexual reproduction under laboratory conditions by a transfer to artificial pond water and increasing the light intensity to 184 mu mol photons m(-2) s(-1). This, however was only possible in early spring, suggesting that the isolated cultures had an internal rhythm. The reproductive morphology was characterized by light- and transmission electron microscopy, and the latter allowed the detection of distinctly oriented microfibrils in the exo- and endospore, and an electron-dense mesospore. Glycan microarray profiling showed that Spirogyra cell walls are rich in major pectic and hemicellulosic polysaccharides, and immuno-fluorescence allowed the detection of arabinogalactan proteins (AGPs) and xyloglucan in the zygospore cell walls. Confocal RAMAN spectroscopy detected complex aromatic compounds, similar in their spectral signature to that of Lycopodium spores. These data support the idea that sexual reproduction in Zygnematophyceae, the sister lineage to land plants, might have played an important role in the process of terrestrialization. Article in Journal/Newspaper Antarc* Antarctic University of Copenhagen: Research Antarctic Plants 10 8 1740 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
alpine region cell wall conjugation Spirogyra sexual reproduction streptophyte zygospore GREEN-ALGA ZYGNEMA FRESH-WATER TERRESTRIAL ALGAE ANTARCTIC STRAINS NY-ALESUND STREPTOPHYTA DIVERSITY EVOLUTION PHOTOSYNTHESIS |
spellingShingle |
alpine region cell wall conjugation Spirogyra sexual reproduction streptophyte zygospore GREEN-ALGA ZYGNEMA FRESH-WATER TERRESTRIAL ALGAE ANTARCTIC STRAINS NY-ALESUND STREPTOPHYTA DIVERSITY EVOLUTION PHOTOSYNTHESIS Permann, Charlotte Herburger, Klaus Felhofer, Martin Gierlinger, Notburga Lewis, Louise A. Holzinger, Andreas Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
topic_facet |
alpine region cell wall conjugation Spirogyra sexual reproduction streptophyte zygospore GREEN-ALGA ZYGNEMA FRESH-WATER TERRESTRIAL ALGAE ANTARCTIC STRAINS NY-ALESUND STREPTOPHYTA DIVERSITY EVOLUTION PHOTOSYNTHESIS |
description |
Extreme environments, such as alpine habitats at high elevation, are increasingly exposed to man-made climate change. Zygnematophyceae thriving in these regions possess a special means of sexual reproduction, termed conjugation, leading to the formation of resistant zygospores. A field sample of Spirogyra with numerous conjugating stages was isolated and characterized by molecular phylogeny. We successfully induced sexual reproduction under laboratory conditions by a transfer to artificial pond water and increasing the light intensity to 184 mu mol photons m(-2) s(-1). This, however was only possible in early spring, suggesting that the isolated cultures had an internal rhythm. The reproductive morphology was characterized by light- and transmission electron microscopy, and the latter allowed the detection of distinctly oriented microfibrils in the exo- and endospore, and an electron-dense mesospore. Glycan microarray profiling showed that Spirogyra cell walls are rich in major pectic and hemicellulosic polysaccharides, and immuno-fluorescence allowed the detection of arabinogalactan proteins (AGPs) and xyloglucan in the zygospore cell walls. Confocal RAMAN spectroscopy detected complex aromatic compounds, similar in their spectral signature to that of Lycopodium spores. These data support the idea that sexual reproduction in Zygnematophyceae, the sister lineage to land plants, might have played an important role in the process of terrestrialization. |
format |
Article in Journal/Newspaper |
author |
Permann, Charlotte Herburger, Klaus Felhofer, Martin Gierlinger, Notburga Lewis, Louise A. Holzinger, Andreas |
author_facet |
Permann, Charlotte Herburger, Klaus Felhofer, Martin Gierlinger, Notburga Lewis, Louise A. Holzinger, Andreas |
author_sort |
Permann, Charlotte |
title |
Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
title_short |
Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
title_full |
Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
title_fullStr |
Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
title_full_unstemmed |
Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta):Advantage under Climate Change Scenarios? |
title_sort |
induction of conjugation and zygospore cell wall characteristics in the alpine spirogyra mirabilis (zygnematophyceae, charophyta):advantage under climate change scenarios? |
publishDate |
2021 |
url |
https://curis.ku.dk/portal/da/publications/induction-of-conjugation-and-zygospore-cell-wall-characteristics-in-the-alpine-spirogyra-mirabilis-zygnematophyceae-charophyta(ce4e3d24-5e7f-427c-8bac-785f649418d6).html https://doi.org/10.3390/plants10081740 https://curis.ku.dk/ws/files/279256591/plants_10_01740_v2.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Permann , C , Herburger , K , Felhofer , M , Gierlinger , N , Lewis , L A & Holzinger , A 2021 , ' Induction of Conjugation and Zygospore Cell Wall Characteristics in the Alpine Spirogyra mirabilis (Zygnematophyceae, Charophyta) : Advantage under Climate Change Scenarios? ' , Plants , vol. 10 , no. 8 , 1740 . https://doi.org/10.3390/plants10081740 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/plants10081740 |
container_title |
Plants |
container_volume |
10 |
container_issue |
8 |
container_start_page |
1740 |
_version_ |
1801374935900225536 |