Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil
Udgivelsesdato: June 2009 Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with 13 C-l...
Published in: | Applied and Environmental Microbiology |
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Online Access: | https://curis.ku.dk/portal/da/publications/differential-utilization-of-carbon-substrates-by-bacteria-and-fungi-in-tundra-soil(c9b99670-a53f-11df-928f-000ea68e967b).html https://doi.org/10.1128/AEM.02865-08 |
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ftcopenhagenunip:oai:pure.atira.dk:publications/c9b99670-a53f-11df-928f-000ea68e967b 2023-05-15T14:59:54+02:00 Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil Rinnan, Riikka Bååth, Erland 2009 https://curis.ku.dk/portal/da/publications/differential-utilization-of-carbon-substrates-by-bacteria-and-fungi-in-tundra-soil(c9b99670-a53f-11df-928f-000ea68e967b).html https://doi.org/10.1128/AEM.02865-08 eng eng info:eu-repo/semantics/restrictedAccess Rinnan , R & Bååth , E 2009 , ' Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil ' , Applied and Environmental Microbiology , vol. 75 , no. 11 , pp. 3611–3620 . https://doi.org/10.1128/AEM.02865-08 article 2009 ftcopenhagenunip https://doi.org/10.1128/AEM.02865-08 2022-02-24T00:05:13Z Udgivelsesdato: June 2009 Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with 13 C-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of 13 C into different phospholipid fatty acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; indicative of fungal storage) was measured after 1 and 7 days. The use of 13 C-labeled substrates allowed the addition of substrates at concentrations low enough not to affect the total amount of PLFA. The label of glucose and acetic acid was rapidly incorporated into the PLFA in a pattern largely corresponding to the fatty acid concentration profile, while glycine and especially starch were mainly taken up by bacteria and not fungi, showing that different groups of the microbial community were responsible for substrate utilization. The 13 C-incorporation from the complex substrates (starch and vanillin) increased over time. There was significant allocation of 13 C into the fungal NLFA, except for starch. For glucose, acetic acid, and glycine, the allocation decreased over time, indicating use of the storage products, whereas for vanillin incorporation into fungal NLFA increased during the incubation. In addition to providing information on functioning of the microbial communities in an arctic soil, our study showed that the combination of PLFA and NLFA analyses yields additional information on the dynamics of substrate degradation. Article in Journal/Newspaper Arctic Subarctic Tundra University of Copenhagen: Research Arctic Applied and Environmental Microbiology 75 11 3611 3620 |
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Open Polar |
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University of Copenhagen: Research |
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ftcopenhagenunip |
language |
English |
description |
Udgivelsesdato: June 2009 Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with 13 C-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of 13 C into different phospholipid fatty acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; indicative of fungal storage) was measured after 1 and 7 days. The use of 13 C-labeled substrates allowed the addition of substrates at concentrations low enough not to affect the total amount of PLFA. The label of glucose and acetic acid was rapidly incorporated into the PLFA in a pattern largely corresponding to the fatty acid concentration profile, while glycine and especially starch were mainly taken up by bacteria and not fungi, showing that different groups of the microbial community were responsible for substrate utilization. The 13 C-incorporation from the complex substrates (starch and vanillin) increased over time. There was significant allocation of 13 C into the fungal NLFA, except for starch. For glucose, acetic acid, and glycine, the allocation decreased over time, indicating use of the storage products, whereas for vanillin incorporation into fungal NLFA increased during the incubation. In addition to providing information on functioning of the microbial communities in an arctic soil, our study showed that the combination of PLFA and NLFA analyses yields additional information on the dynamics of substrate degradation. |
format |
Article in Journal/Newspaper |
author |
Rinnan, Riikka Bååth, Erland |
spellingShingle |
Rinnan, Riikka Bååth, Erland Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
author_facet |
Rinnan, Riikka Bååth, Erland |
author_sort |
Rinnan, Riikka |
title |
Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
title_short |
Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
title_full |
Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
title_fullStr |
Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
title_full_unstemmed |
Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil |
title_sort |
differential utilization of carbon substrates by bacteria and fungi in tundra soil |
publishDate |
2009 |
url |
https://curis.ku.dk/portal/da/publications/differential-utilization-of-carbon-substrates-by-bacteria-and-fungi-in-tundra-soil(c9b99670-a53f-11df-928f-000ea68e967b).html https://doi.org/10.1128/AEM.02865-08 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Subarctic Tundra |
genre_facet |
Arctic Subarctic Tundra |
op_source |
Rinnan , R & Bååth , E 2009 , ' Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil ' , Applied and Environmental Microbiology , vol. 75 , no. 11 , pp. 3611–3620 . https://doi.org/10.1128/AEM.02865-08 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1128/AEM.02865-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
75 |
container_issue |
11 |
container_start_page |
3611 |
op_container_end_page |
3620 |
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1766332024372592640 |