A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer
The present study compared O 2 exchange measurements, fluorometry and 14 c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O 2 , while the melting water released during thawing is u...
Published in: | Aquatic Microbial Ecology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2002
|
Subjects: | |
Online Access: | https://curis.ku.dk/portal/da/publications/a-laboratory-study-on-o2-dynamics-and-photosynthesis-in-ice-algal-communities-quantification-by-microsensors-o2-exchange-rates-14c-incubations-and-a-pam-fluorometer(ae01e260-74c5-11db-bee9-02004c4f4f50).html https://doi.org/10.3354/ame027301 http://www.scopus.com/inward/record.url?scp=0037117811&partnerID=8YFLogxK |
id |
ftcopenhagenunip:oai:pure.atira.dk:publications/ae01e260-74c5-11db-bee9-02004c4f4f50 |
---|---|
record_format |
openpolar |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/ae01e260-74c5-11db-bee9-02004c4f4f50 2023-12-24T10:17:27+01:00 A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer Glud, Ronnie N. Rysgaard, Søren Kühl, Michael 2002-04-16 https://curis.ku.dk/portal/da/publications/a-laboratory-study-on-o2-dynamics-and-photosynthesis-in-ice-algal-communities-quantification-by-microsensors-o2-exchange-rates-14c-incubations-and-a-pam-fluorometer(ae01e260-74c5-11db-bee9-02004c4f4f50).html https://doi.org/10.3354/ame027301 http://www.scopus.com/inward/record.url?scp=0037117811&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Glud , R N , Rysgaard , S & Kühl , M 2002 , ' A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer : Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer ' , Aquatic Microbial Ecology , vol. 27 , no. 3 , pp. 301-311 . https://doi.org/10.3354/ame027301 Fluorometer Microelectrodes Oxygen Primary production Pulse amplitude modulated fluorometry Sea-ice algae article 2002 ftcopenhagenunip https://doi.org/10.3354/ame027301 2023-11-29T23:58:22Z The present study compared O 2 exchange measurements, fluorometry and 14 c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O 2 , while the melting water released during thawing is undersaturated with O 2 . This results in a highly variable O 2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O 2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement of O 2 concentrations in water below sea ice. Accounting for the O 2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O 2 exchange rates and 14 C incubation, co-varied as a function of biomass and irradiance. The ratio between the net O 2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 ± 0.48. Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F 0 ) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities. A similar observation was made for the correlation between measurements and the concentration of chlorophyll a. Article in Journal/Newspaper ice algae Sea ice University of Copenhagen: Research Aquatic Microbial Ecology 27 301 311 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Fluorometer Microelectrodes Oxygen Primary production Pulse amplitude modulated fluorometry Sea-ice algae |
spellingShingle |
Fluorometer Microelectrodes Oxygen Primary production Pulse amplitude modulated fluorometry Sea-ice algae Glud, Ronnie N. Rysgaard, Søren Kühl, Michael A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
topic_facet |
Fluorometer Microelectrodes Oxygen Primary production Pulse amplitude modulated fluorometry Sea-ice algae |
description |
The present study compared O 2 exchange measurements, fluorometry and 14 c incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O 2 , while the melting water released during thawing is undersaturated with O 2 . This results in a highly variable O 2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O 2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement of O 2 concentrations in water below sea ice. Accounting for the O 2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O 2 exchange rates and 14 C incubation, co-varied as a function of biomass and irradiance. The ratio between the net O 2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 ± 0.48. Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F 0 ) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities. A similar observation was made for the correlation between measurements and the concentration of chlorophyll a. |
format |
Article in Journal/Newspaper |
author |
Glud, Ronnie N. Rysgaard, Søren Kühl, Michael |
author_facet |
Glud, Ronnie N. Rysgaard, Søren Kühl, Michael |
author_sort |
Glud, Ronnie N. |
title |
A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
title_short |
A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
title_full |
A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
title_fullStr |
A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
title_full_unstemmed |
A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer:Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer |
title_sort |
laboratory study on o 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, o 2 exchange rates, 14 c incubations and a pam fluorometer:quantification by microsensors, o 2 exchange rates, 14 c incubations and a pam fluorometer |
publishDate |
2002 |
url |
https://curis.ku.dk/portal/da/publications/a-laboratory-study-on-o2-dynamics-and-photosynthesis-in-ice-algal-communities-quantification-by-microsensors-o2-exchange-rates-14c-incubations-and-a-pam-fluorometer(ae01e260-74c5-11db-bee9-02004c4f4f50).html https://doi.org/10.3354/ame027301 http://www.scopus.com/inward/record.url?scp=0037117811&partnerID=8YFLogxK |
genre |
ice algae Sea ice |
genre_facet |
ice algae Sea ice |
op_source |
Glud , R N , Rysgaard , S & Kühl , M 2002 , ' A laboratory study on O 2 dynamics and photosynthesis in ice algal communities: quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer : Quantification by microsensors, O 2 exchange rates, 14 C incubations and a PAM fluorometer ' , Aquatic Microbial Ecology , vol. 27 , no. 3 , pp. 301-311 . https://doi.org/10.3354/ame027301 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.3354/ame027301 |
container_title |
Aquatic Microbial Ecology |
container_volume |
27 |
container_start_page |
301 |
op_container_end_page |
311 |
_version_ |
1786205637055610880 |