Spectrophotometric retinal oximetry in pigs
PURPOSE: To assess the validity of spectrophotometric retinal oximetry, by comparison to blood gas analysis and intra-vitreal measurements of partial pressure of oxygen (pO2). METHODS: Female domestic pigs were used for all experiments (n=8). Oxygen fraction in inspired air was changed using a mixtu...
Published in: | Investigative Opthalmology & Visual Science |
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2013
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ftcopenhagenunip:oai:pure.atira.dk:publications/abf158f7-f4c4-417f-8d5e-735dbade36d5 2023-12-17T10:32:15+01:00 Spectrophotometric retinal oximetry in pigs Traustason, Sindri Kiilgaard, Jens Folke Karlsson, Robert Hardarson, Sveinn H Stefansson, Einar la Cour, Morten 2013 https://curis.ku.dk/portal/da/publications/spectrophotometric-retinal-oximetry-in-pigs(abf158f7-f4c4-417f-8d5e-735dbade36d5).html https://doi.org/10.1167/iovs.12-11284 eng eng info:eu-repo/semantics/restrictedAccess Traustason , S , Kiilgaard , J F , Karlsson , R , Hardarson , S H , Stefansson , E & la Cour , M 2013 , ' Spectrophotometric retinal oximetry in pigs ' , Investigative Ophthalmology & Visual Science . https://doi.org/10.1167/iovs.12-11284 article 2013 ftcopenhagenunip https://doi.org/10.1167/iovs.12-11284 2023-11-22T23:59:26Z PURPOSE: To assess the validity of spectrophotometric retinal oximetry, by comparison to blood gas analysis and intra-vitreal measurements of partial pressure of oxygen (pO2). METHODS: Female domestic pigs were used for all experiments (n=8). Oxygen fraction in inspired air was changed using a mixture of room air, pure oxygen and pure nitrogen, ranging from 5% to 100% oxygen. Femoral arterial blood gas analysis and retinal oximetry was performed at each level of inspiratory oxygen fraction. Retinal oximetry was performed using a commercial instrument, the Oxymap Retinal Oximeter T1 (Oxymap ehf, Reykjavik, Iceland). The device simultaneously acquires images at two wavelengths (570 nm and 600 nm) and specialized software automatically detects retinal blood vessels. In three pigs, invasive pO2-measurements were performed after the initial non-invasive measurements. RESULTS: Comparison of femoral arterial oxygen saturation and the optical density ratio over retinal arteries revealed an approximately linear relationship (R(2) = 0.74, p = 3.4 x 10(-9)). In order to test the validity of applying the arterial calibration to veins, we compared non-invasive oximetry measurements to invasive pO2-measurements in three pigs. This relationship was approximately linear (R(2) = 0.45, p = 0.04). CONCLUSIONS: Non-invasive spectrophotometric oximetry is sensitive to changes in oxygen saturation in pigs and correlated with intravitreal pO2-measurements and with femoral artery pO2. Pigs present a higher intra-individual variability in retinal oxygen saturation and a lower overall saturation than humans. The difference between porcine and human eyes makes direct comparisons of measurements difficult. Article in Journal/Newspaper Iceland University of Copenhagen: Research Investigative Opthalmology & Visual Science 54 4 2746 |
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Open Polar |
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University of Copenhagen: Research |
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ftcopenhagenunip |
language |
English |
description |
PURPOSE: To assess the validity of spectrophotometric retinal oximetry, by comparison to blood gas analysis and intra-vitreal measurements of partial pressure of oxygen (pO2). METHODS: Female domestic pigs were used for all experiments (n=8). Oxygen fraction in inspired air was changed using a mixture of room air, pure oxygen and pure nitrogen, ranging from 5% to 100% oxygen. Femoral arterial blood gas analysis and retinal oximetry was performed at each level of inspiratory oxygen fraction. Retinal oximetry was performed using a commercial instrument, the Oxymap Retinal Oximeter T1 (Oxymap ehf, Reykjavik, Iceland). The device simultaneously acquires images at two wavelengths (570 nm and 600 nm) and specialized software automatically detects retinal blood vessels. In three pigs, invasive pO2-measurements were performed after the initial non-invasive measurements. RESULTS: Comparison of femoral arterial oxygen saturation and the optical density ratio over retinal arteries revealed an approximately linear relationship (R(2) = 0.74, p = 3.4 x 10(-9)). In order to test the validity of applying the arterial calibration to veins, we compared non-invasive oximetry measurements to invasive pO2-measurements in three pigs. This relationship was approximately linear (R(2) = 0.45, p = 0.04). CONCLUSIONS: Non-invasive spectrophotometric oximetry is sensitive to changes in oxygen saturation in pigs and correlated with intravitreal pO2-measurements and with femoral artery pO2. Pigs present a higher intra-individual variability in retinal oxygen saturation and a lower overall saturation than humans. The difference between porcine and human eyes makes direct comparisons of measurements difficult. |
format |
Article in Journal/Newspaper |
author |
Traustason, Sindri Kiilgaard, Jens Folke Karlsson, Robert Hardarson, Sveinn H Stefansson, Einar la Cour, Morten |
spellingShingle |
Traustason, Sindri Kiilgaard, Jens Folke Karlsson, Robert Hardarson, Sveinn H Stefansson, Einar la Cour, Morten Spectrophotometric retinal oximetry in pigs |
author_facet |
Traustason, Sindri Kiilgaard, Jens Folke Karlsson, Robert Hardarson, Sveinn H Stefansson, Einar la Cour, Morten |
author_sort |
Traustason, Sindri |
title |
Spectrophotometric retinal oximetry in pigs |
title_short |
Spectrophotometric retinal oximetry in pigs |
title_full |
Spectrophotometric retinal oximetry in pigs |
title_fullStr |
Spectrophotometric retinal oximetry in pigs |
title_full_unstemmed |
Spectrophotometric retinal oximetry in pigs |
title_sort |
spectrophotometric retinal oximetry in pigs |
publishDate |
2013 |
url |
https://curis.ku.dk/portal/da/publications/spectrophotometric-retinal-oximetry-in-pigs(abf158f7-f4c4-417f-8d5e-735dbade36d5).html https://doi.org/10.1167/iovs.12-11284 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Traustason , S , Kiilgaard , J F , Karlsson , R , Hardarson , S H , Stefansson , E & la Cour , M 2013 , ' Spectrophotometric retinal oximetry in pigs ' , Investigative Ophthalmology & Visual Science . https://doi.org/10.1167/iovs.12-11284 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1167/iovs.12-11284 |
container_title |
Investigative Opthalmology & Visual Science |
container_volume |
54 |
container_issue |
4 |
container_start_page |
2746 |
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1785585806424932352 |