Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion
Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBDGA) or Arabidopsis thaliana glucan, water dikinase...
Published in: | Journal of Agricultural and Food Chemistry |
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2023
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Online Access: | https://curis.ku.dk/portal/da/publications/improved-hydrolysis-of-granular-starches-by-a-psychrophilic-amylase-starch-binding-domainfusion(9c6bbf22-ce6c-4111-9425-681b686b2076).html https://doi.org/10.1021/acs.jafc.3c01898 |
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ftcopenhagenunip:oai:pure.atira.dk:publications/9c6bbf22-ce6c-4111-9425-681b686b2076 2024-06-09T07:39:55+00:00 Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte 2023 https://curis.ku.dk/portal/da/publications/improved-hydrolysis-of-granular-starches-by-a-psychrophilic-amylase-starch-binding-domainfusion(9c6bbf22-ce6c-4111-9425-681b686b2076).html https://doi.org/10.1021/acs.jafc.3c01898 eng eng info:eu-repo/semantics/closedAccess Wang , Y , Tian , Y , Zhong , Y , Suleiman , M A , Feller , G , Westh , P , Blennow , A , Møller , M S & Svensson , B 2023 , ' Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion ' , Journal of Agricultural and Food Chemistry , vol. 71 , no. 23 , pp. 9040-9050 . https://doi.org/10.1021/acs.jafc.3c01898 alpha-Amylases/chemistry Hydrolysis Protein Structure Tertiary Starch/chemistry Amylose/chemistry article 2023 ftcopenhagenunip https://doi.org/10.1021/acs.jafc.3c01898 2024-05-16T11:29:29Z Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBDGA) or Arabidopsis thaliana glucan, water dikinase 3 (SBDGWD3) via a decapeptide linker. Depending on the waxy, normal or high-amylose starch type and the botanical source, the AHA-SBD fusion enzymes showed up to 3 times higher activity than AHA wild-type. The SBD-fusion thus increased the density of enzyme attack-sites and binding-sites on the starch granules by up to 5- and 7-fold, respectively, as measured using an interfacial catalysis approach that combined conventional Michaelis-Menten kinetics, with the substrate in excess, and inverse kinetics, having enzyme in excess, with enzyme-starch granule adsorption isotherms. Higher substrate affinity of the SBDGA compared to SBDGWD3 was accompanied by the superior activity of AHA-SBDGA in agreement with the Sabatier principle of adsorption limited heterogenous catalysis. Article in Journal/Newspaper Antarc* Antarctic University of Copenhagen: Research Antarctic The Antarctic Journal of Agricultural and Food Chemistry 71 23 9040 9050 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
alpha-Amylases/chemistry Hydrolysis Protein Structure Tertiary Starch/chemistry Amylose/chemistry |
spellingShingle |
alpha-Amylases/chemistry Hydrolysis Protein Structure Tertiary Starch/chemistry Amylose/chemistry Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
topic_facet |
alpha-Amylases/chemistry Hydrolysis Protein Structure Tertiary Starch/chemistry Amylose/chemistry |
description |
Degradation of starch granules by a psychrophilic α-amylase, AHA, from the Antarctic bacterium Pseudoalteromonas haloplanktis TAB23 was facilitated by C-terminal fusion to a starch-binding domain (SBD) from either Aspergillus niger glucoamylase (SBDGA) or Arabidopsis thaliana glucan, water dikinase 3 (SBDGWD3) via a decapeptide linker. Depending on the waxy, normal or high-amylose starch type and the botanical source, the AHA-SBD fusion enzymes showed up to 3 times higher activity than AHA wild-type. The SBD-fusion thus increased the density of enzyme attack-sites and binding-sites on the starch granules by up to 5- and 7-fold, respectively, as measured using an interfacial catalysis approach that combined conventional Michaelis-Menten kinetics, with the substrate in excess, and inverse kinetics, having enzyme in excess, with enzyme-starch granule adsorption isotherms. Higher substrate affinity of the SBDGA compared to SBDGWD3 was accompanied by the superior activity of AHA-SBDGA in agreement with the Sabatier principle of adsorption limited heterogenous catalysis. |
format |
Article in Journal/Newspaper |
author |
Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte |
author_facet |
Wang, Yu Tian, Yu Zhong, Yuyue Suleiman, Mohammad Amer Feller, Georges Westh, Peter Blennow, Andreas Møller, Marie Sofie Svensson, Birte |
author_sort |
Wang, Yu |
title |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_short |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_full |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_fullStr |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_full_unstemmed |
Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion |
title_sort |
improved hydrolysis of granular starches by a psychrophilic α-amylase starch binding domain-fusion |
publishDate |
2023 |
url |
https://curis.ku.dk/portal/da/publications/improved-hydrolysis-of-granular-starches-by-a-psychrophilic-amylase-starch-binding-domainfusion(9c6bbf22-ce6c-4111-9425-681b686b2076).html https://doi.org/10.1021/acs.jafc.3c01898 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Wang , Y , Tian , Y , Zhong , Y , Suleiman , M A , Feller , G , Westh , P , Blennow , A , Møller , M S & Svensson , B 2023 , ' Improved Hydrolysis of Granular Starches by a Psychrophilic α-Amylase Starch Binding Domain-Fusion ' , Journal of Agricultural and Food Chemistry , vol. 71 , no. 23 , pp. 9040-9050 . https://doi.org/10.1021/acs.jafc.3c01898 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1021/acs.jafc.3c01898 |
container_title |
Journal of Agricultural and Food Chemistry |
container_volume |
71 |
container_issue |
23 |
container_start_page |
9040 |
op_container_end_page |
9050 |
_version_ |
1801383188871774208 |