Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil
The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during warming and freezing of...
Published in: | The ISME Journal |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://curis.ku.dk/portal/da/publications/bacterial-and-protozoan-dynamics-upon-thawing-and-freezing-of-an-active-layer-permafrost-soil(99b0ae45-a5d9-4524-ae5a-9da65029696a).html https://doi.org/10.1038/s41396-019-0351-x https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474304/pdf/41396_2019_Article_351.pdf |
id |
ftcopenhagenunip:oai:pure.atira.dk:publications/99b0ae45-a5d9-4524-ae5a-9da65029696a |
---|---|
record_format |
openpolar |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/99b0ae45-a5d9-4524-ae5a-9da65029696a 2023-12-17T10:26:24+01:00 Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil Schostag, Morten Priemé, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr 2019 https://curis.ku.dk/portal/da/publications/bacterial-and-protozoan-dynamics-upon-thawing-and-freezing-of-an-active-layer-permafrost-soil(99b0ae45-a5d9-4524-ae5a-9da65029696a).html https://doi.org/10.1038/s41396-019-0351-x https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474304/pdf/41396_2019_Article_351.pdf eng eng info:eu-repo/semantics/openAccess Schostag , M , Priemé , A , Jacquiod , S , Russel , J , Ekelund , F & Jacobsen , C S 2019 , ' Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil ' , ISME Journal , vol. 13 , pp. 1345-1359 . https://doi.org/10.1038/s41396-019-0351-x article 2019 ftcopenhagenunip https://doi.org/10.1038/s41396-019-0351-x 2023-11-23T00:04:20Z The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during warming and freezing of the active layer soil from Svalbard, Norway. Soil community data were obtained by direct shotgun sequencing of total extracted RNA. No changes in soil microbial communities were detected when warming from −10 to −2 °C or when freezing from −2 to −10 °C. In contrast, within a few days we observed changes when warming from −2 to +2 °C with a decrease in fungal rRNA and an increase in several OTUs belonging to Gemmatimonadetes, Bacteroidetes and Betaproteobacteria. Even more substantial changes occurred when incubating at 2 °C for 16 days, with declines in total fungal potential activity and decreases in oligotrophic members from Actinobacteria and Acidobacteria. Additionally, we detected an increase in transcriptome sequences of bacterial phyla Bacteriodetes, Firmicutes, Betaproteobacteria and Gammaproteobacteria—collectively presumed to be copiotrophic. Furthermore, we detected an increase in putative bacterivorous heterotrophic flagellates, likely due to predation upon the bacterial community via grazing. Although this grazing activity may explain relatively large changes in the bacterial community composition, no changes in total 16S rRNA gene copy number were observed and the total RNA level remained stable during the incubation. Together, these results are showing the first comprehensive ecological evaluation across prokaryotic and eukaryotic microbial communities on thawing and freezing of soil by application of the TotalRNA technique. Article in Journal/Newspaper Arctic permafrost Svalbard University of Copenhagen: Research Arctic Svalbard Norway The ISME Journal 13 5 1345 1359 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
description |
The active layer of soil overlaying permafrost in the Arctic is subjected to annual changes in temperature and soil chemistry, which we hypothesize to affect the overall soil microbial community. We investigated changes in soil microorganisms at different temperatures during warming and freezing of the active layer soil from Svalbard, Norway. Soil community data were obtained by direct shotgun sequencing of total extracted RNA. No changes in soil microbial communities were detected when warming from −10 to −2 °C or when freezing from −2 to −10 °C. In contrast, within a few days we observed changes when warming from −2 to +2 °C with a decrease in fungal rRNA and an increase in several OTUs belonging to Gemmatimonadetes, Bacteroidetes and Betaproteobacteria. Even more substantial changes occurred when incubating at 2 °C for 16 days, with declines in total fungal potential activity and decreases in oligotrophic members from Actinobacteria and Acidobacteria. Additionally, we detected an increase in transcriptome sequences of bacterial phyla Bacteriodetes, Firmicutes, Betaproteobacteria and Gammaproteobacteria—collectively presumed to be copiotrophic. Furthermore, we detected an increase in putative bacterivorous heterotrophic flagellates, likely due to predation upon the bacterial community via grazing. Although this grazing activity may explain relatively large changes in the bacterial community composition, no changes in total 16S rRNA gene copy number were observed and the total RNA level remained stable during the incubation. Together, these results are showing the first comprehensive ecological evaluation across prokaryotic and eukaryotic microbial communities on thawing and freezing of soil by application of the TotalRNA technique. |
format |
Article in Journal/Newspaper |
author |
Schostag, Morten Priemé, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr |
spellingShingle |
Schostag, Morten Priemé, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
author_facet |
Schostag, Morten Priemé, Anders Jacquiod, Samuel Russel, Jakob Ekelund, Flemming Jacobsen, Carsten Suhr |
author_sort |
Schostag, Morten |
title |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_short |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_full |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_fullStr |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_full_unstemmed |
Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
title_sort |
bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil |
publishDate |
2019 |
url |
https://curis.ku.dk/portal/da/publications/bacterial-and-protozoan-dynamics-upon-thawing-and-freezing-of-an-active-layer-permafrost-soil(99b0ae45-a5d9-4524-ae5a-9da65029696a).html https://doi.org/10.1038/s41396-019-0351-x https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474304/pdf/41396_2019_Article_351.pdf |
geographic |
Arctic Svalbard Norway |
geographic_facet |
Arctic Svalbard Norway |
genre |
Arctic permafrost Svalbard |
genre_facet |
Arctic permafrost Svalbard |
op_source |
Schostag , M , Priemé , A , Jacquiod , S , Russel , J , Ekelund , F & Jacobsen , C S 2019 , ' Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil ' , ISME Journal , vol. 13 , pp. 1345-1359 . https://doi.org/10.1038/s41396-019-0351-x |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41396-019-0351-x |
container_title |
The ISME Journal |
container_volume |
13 |
container_issue |
5 |
container_start_page |
1345 |
op_container_end_page |
1359 |
_version_ |
1785578116652990464 |