Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks
CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing...
Published in: | Veterinary Microbiology |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | https://curis.ku.dk/portal/da/publications/genotyping-canine-distemper-virus-cdv-by-a-heminested-multiplex-pcr-provides-a-rapid-approach-for-investigation-of-cdv-outbreaks(9838f5a0-a1c2-11dd-b6ae-000ea68e967b).html https://doi.org/10.1016/j.vetmic.2007.01.005 |
id |
ftcopenhagenunip:oai:pure.atira.dk:publications/9838f5a0-a1c2-11dd-b6ae-000ea68e967b |
---|---|
record_format |
openpolar |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/9838f5a0-a1c2-11dd-b6ae-000ea68e967b 2024-01-14T10:04:42+01:00 Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks Blixenkrone-Møller, Merete Martella, Vito 2007 https://curis.ku.dk/portal/da/publications/genotyping-canine-distemper-virus-cdv-by-a-heminested-multiplex-pcr-provides-a-rapid-approach-for-investigation-of-cdv-outbreaks(9838f5a0-a1c2-11dd-b6ae-000ea68e967b).html https://doi.org/10.1016/j.vetmic.2007.01.005 eng eng info:eu-repo/semantics/closedAccess Blixenkrone-Møller , M & Martella , V 2007 , ' Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks ' , Veterinary Microbiology , vol. 122 , no. 1-2 , pp. 32-42 . https://doi.org/10.1016/j.vetmic.2007.01.005 /dk/atira/pure/core/keywords/Life Former LIFE faculty Hundesygevirus genotypning Canine distemper virus genotyping article 2007 ftcopenhagenunip https://doi.org/10.1016/j.vetmic.2007.01.005 2023-12-20T23:58:37Z CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-naïve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease. Article in Journal/Newspaper Arctic University of Copenhagen: Research Arctic Veterinary Microbiology 122 1-2 32 42 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
/dk/atira/pure/core/keywords/Life Former LIFE faculty Hundesygevirus genotypning Canine distemper virus genotyping |
spellingShingle |
/dk/atira/pure/core/keywords/Life Former LIFE faculty Hundesygevirus genotypning Canine distemper virus genotyping Blixenkrone-Møller, Merete Martella, Vito Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
topic_facet |
/dk/atira/pure/core/keywords/Life Former LIFE faculty Hundesygevirus genotypning Canine distemper virus genotyping |
description |
CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-naïve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease. |
format |
Article in Journal/Newspaper |
author |
Blixenkrone-Møller, Merete Martella, Vito |
author_facet |
Blixenkrone-Møller, Merete Martella, Vito |
author_sort |
Blixenkrone-Møller, Merete |
title |
Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
title_short |
Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
title_full |
Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
title_fullStr |
Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
title_full_unstemmed |
Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks |
title_sort |
genotyping canine distemper virus (cdv) by a hemi-nested multiplex pcr provides a rapid approach for investigation of cdv outbreaks |
publishDate |
2007 |
url |
https://curis.ku.dk/portal/da/publications/genotyping-canine-distemper-virus-cdv-by-a-heminested-multiplex-pcr-provides-a-rapid-approach-for-investigation-of-cdv-outbreaks(9838f5a0-a1c2-11dd-b6ae-000ea68e967b).html https://doi.org/10.1016/j.vetmic.2007.01.005 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Blixenkrone-Møller , M & Martella , V 2007 , ' Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks ' , Veterinary Microbiology , vol. 122 , no. 1-2 , pp. 32-42 . https://doi.org/10.1016/j.vetmic.2007.01.005 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.vetmic.2007.01.005 |
container_title |
Veterinary Microbiology |
container_volume |
122 |
container_issue |
1-2 |
container_start_page |
32 |
op_container_end_page |
42 |
_version_ |
1788059185381900288 |