A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa
We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of...
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2013
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Online Access: | https://curis.ku.dk/portal/da/publications/a-comparative-study-of-ancient-environmental-dna-to-pollen-and-macrofossils-from-lake-sediments-reveals-taxonomic-overlap-and-additional-plant-taxa(87092c06-4a81-4fd8-855a-4e74b712c946).html https://doi.org/10.1016/j.quascirev.2013.06.006 http://www.scopus.com/inward/record.url?scp=84880356631&partnerID=8YFLogxK |
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ftcopenhagenunip:oai:pure.atira.dk:publications/87092c06-4a81-4fd8-855a-4e74b712c946 2024-05-19T07:36:15+00:00 A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa Pedersen, Mikkel Winther Ginolhac, Aurélien Orlando, Ludovic Antoine Alexandre Olsen, Jesper Andersen, Kenneth Holm, Jakob Mørkøv Funder, Svend Visby Willerslev, Eske Kjær, Kurt H. 2013-09-01 https://curis.ku.dk/portal/da/publications/a-comparative-study-of-ancient-environmental-dna-to-pollen-and-macrofossils-from-lake-sediments-reveals-taxonomic-overlap-and-additional-plant-taxa(87092c06-4a81-4fd8-855a-4e74b712c946).html https://doi.org/10.1016/j.quascirev.2013.06.006 http://www.scopus.com/inward/record.url?scp=84880356631&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Pedersen , M W , Ginolhac , A , Orlando , L A A , Olsen , J , Andersen , K , Holm , J M , Funder , S V , Willerslev , E & Kjær , K H 2013 , ' A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa ' , Quaternary Science Reviews , vol. 75 , pp. 161-168 . https://doi.org/10.1016/j.quascirev.2013.06.006 article 2013 ftcopenhagenunip https://doi.org/10.1016/j.quascirev.2013.06.006 2024-04-25T00:45:01Z We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa. Article in Journal/Newspaper Arctic Greenland University of Copenhagen: Research Quaternary Science Reviews 75 161 168 |
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Open Polar |
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University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
description |
We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa. |
format |
Article in Journal/Newspaper |
author |
Pedersen, Mikkel Winther Ginolhac, Aurélien Orlando, Ludovic Antoine Alexandre Olsen, Jesper Andersen, Kenneth Holm, Jakob Mørkøv Funder, Svend Visby Willerslev, Eske Kjær, Kurt H. |
spellingShingle |
Pedersen, Mikkel Winther Ginolhac, Aurélien Orlando, Ludovic Antoine Alexandre Olsen, Jesper Andersen, Kenneth Holm, Jakob Mørkøv Funder, Svend Visby Willerslev, Eske Kjær, Kurt H. A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
author_facet |
Pedersen, Mikkel Winther Ginolhac, Aurélien Orlando, Ludovic Antoine Alexandre Olsen, Jesper Andersen, Kenneth Holm, Jakob Mørkøv Funder, Svend Visby Willerslev, Eske Kjær, Kurt H. |
author_sort |
Pedersen, Mikkel Winther |
title |
A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
title_short |
A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
title_full |
A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
title_fullStr |
A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
title_full_unstemmed |
A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
title_sort |
comparative study of ancient environmental dna to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa |
publishDate |
2013 |
url |
https://curis.ku.dk/portal/da/publications/a-comparative-study-of-ancient-environmental-dna-to-pollen-and-macrofossils-from-lake-sediments-reveals-taxonomic-overlap-and-additional-plant-taxa(87092c06-4a81-4fd8-855a-4e74b712c946).html https://doi.org/10.1016/j.quascirev.2013.06.006 http://www.scopus.com/inward/record.url?scp=84880356631&partnerID=8YFLogxK |
genre |
Arctic Greenland |
genre_facet |
Arctic Greenland |
op_source |
Pedersen , M W , Ginolhac , A , Orlando , L A A , Olsen , J , Andersen , K , Holm , J M , Funder , S V , Willerslev , E & Kjær , K H 2013 , ' A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa ' , Quaternary Science Reviews , vol. 75 , pp. 161-168 . https://doi.org/10.1016/j.quascirev.2013.06.006 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.quascirev.2013.06.006 |
container_title |
Quaternary Science Reviews |
container_volume |
75 |
container_start_page |
161 |
op_container_end_page |
168 |
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1799475340168396800 |