Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for f...
Published in: | Proceedings of the Royal Society B: Biological Sciences |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2021
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Online Access: | https://curis.ku.dk/portal/da/publications/palaeoproteomic-analyses-of-dog-palaeofaeces-reveal-a-preserved-dietary-and-host-digestive-proteome(77c8d09e-6253-4c04-a777-2c89938de85a).html https://doi.org/10.1098/rspb.2021.0020 https://curis.ku.dk/ws/files/275826280/rspb.2021.0020.pdf |
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ftcopenhagenunip:oai:pure.atira.dk:publications/77c8d09e-6253-4c04-a777-2c89938de85a 2024-06-09T07:44:14+00:00 Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate MacKie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla 2021 application/pdf https://curis.ku.dk/portal/da/publications/palaeoproteomic-analyses-of-dog-palaeofaeces-reveal-a-preserved-dietary-and-host-digestive-proteome(77c8d09e-6253-4c04-a777-2c89938de85a).html https://doi.org/10.1098/rspb.2021.0020 https://curis.ku.dk/ws/files/275826280/rspb.2021.0020.pdf eng eng info:eu-repo/semantics/openAccess Runge , A K W , Hendy , J , Richter , K K , Masson-MacLean , E , Britton , K , MacKie , M , McGrath , K , Collins , M , Cappellini , E & Speller , C 2021 , ' Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome ' , Proceedings of the Royal Society B: Biological Sciences , vol. 288 , no. 1954 , 20210020 . https://doi.org/10.1098/rspb.2021.0020 archaeology dogs Nunalleq Alaska palaeofaeces palaeoproteomics zooms article 2021 ftcopenhagenunip https://doi.org/10.1098/rspb.2021.0020 2024-05-16T11:29:21Z The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. Article in Journal/Newspaper Arctic permafrost Alaska University of Copenhagen: Research Arctic Pacific Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) Proceedings of the Royal Society B: Biological Sciences 288 1954 20210020 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
archaeology dogs Nunalleq Alaska palaeofaeces palaeoproteomics zooms |
spellingShingle |
archaeology dogs Nunalleq Alaska palaeofaeces palaeoproteomics zooms Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate MacKie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
topic_facet |
archaeology dogs Nunalleq Alaska palaeofaeces palaeoproteomics zooms |
description |
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. |
format |
Article in Journal/Newspaper |
author |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate MacKie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_facet |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate MacKie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_sort |
Runge, Anne Kathrine W. |
title |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_short |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_fullStr |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full_unstemmed |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_sort |
palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
publishDate |
2021 |
url |
https://curis.ku.dk/portal/da/publications/palaeoproteomic-analyses-of-dog-palaeofaeces-reveal-a-preserved-dietary-and-host-digestive-proteome(77c8d09e-6253-4c04-a777-2c89938de85a).html https://doi.org/10.1098/rspb.2021.0020 https://curis.ku.dk/ws/files/275826280/rspb.2021.0020.pdf |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Arctic Pacific Sockeye |
geographic_facet |
Arctic Pacific Sockeye |
genre |
Arctic permafrost Alaska |
genre_facet |
Arctic permafrost Alaska |
op_source |
Runge , A K W , Hendy , J , Richter , K K , Masson-MacLean , E , Britton , K , MacKie , M , McGrath , K , Collins , M , Cappellini , E & Speller , C 2021 , ' Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome ' , Proceedings of the Royal Society B: Biological Sciences , vol. 288 , no. 1954 , 20210020 . https://doi.org/10.1098/rspb.2021.0020 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1098/rspb.2021.0020 |
container_title |
Proceedings of the Royal Society B: Biological Sciences |
container_volume |
288 |
container_issue |
1954 |
container_start_page |
20210020 |
_version_ |
1801373014070132736 |