A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry
This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful...
Published in: | Journal of Chromatography B |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2016
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Online Access: | https://curis.ku.dk/portal/da/publications/a-novel-method-for-analysing-key-corticosteroids-in-polar-bear-ursus-maritimus-hair-using-liquid-chromatography-tandem-mass-spectrometry(7250b45a-07b3-4bdd-b8f7-32600e50dd7f).html https://doi.org/10.1016/j.jchromb.2016.02.029 |
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ftcopenhagenunip:oai:pure.atira.dk:publications/7250b45a-07b3-4bdd-b8f7-32600e50dd7f 2024-05-12T08:03:03+00:00 A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry Weisser, Johan J Hansen, Martin Björklund, Erland Sonne, Christian Dietz, Rune Styrishave, Bjarne 2016-04-01 https://curis.ku.dk/portal/da/publications/a-novel-method-for-analysing-key-corticosteroids-in-polar-bear-ursus-maritimus-hair-using-liquid-chromatography-tandem-mass-spectrometry(7250b45a-07b3-4bdd-b8f7-32600e50dd7f).html https://doi.org/10.1016/j.jchromb.2016.02.029 eng eng info:eu-repo/semantics/closedAccess Weisser , J J , Hansen , M , Björklund , E , Sonne , C , Dietz , R & Styrishave , B 2016 , ' A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry ' , Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 1017-1018 , pp. 45-51 . https://doi.org/10.1016/j.jchromb.2016.02.029 Journal Article Research Support Non-U.S. Gov't article 2016 ftcopenhagenunip https://doi.org/10.1016/j.jchromb.2016.02.029 2024-04-18T00:28:13Z This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)–solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC–MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to previous polar bear studies based on ELISA techniques. Absolute method recoveries were 81%, 75% and 60% for cortisol, corticosterone and aldosterone, respectively. We applied the developed method on a hair sample pooled from four East Greenland polar bears. Herein cortisol and corticosterone were successfully determined in levels of 0.32 ± 0.02 ng/g hair and 0.13 ± 0.02 ng/g hair, respectively. Aldosterone was below limit of detection (LOD < 0.17 ng/g). The cortisol hair concentration found in these East Greenland polar bears was consistent with cortisol levels previously determined in the Southern Hudson Bay and James Bay in Canada using ELISA kits. This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)-solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to ... Article in Journal/Newspaper East Greenland Greenland Hudson Bay Ursus maritimus James Bay University of Copenhagen: Research Canada Greenland Hudson Hudson Bay Journal of Chromatography B 1017-1018 45 51 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Journal Article Research Support Non-U.S. Gov't |
spellingShingle |
Journal Article Research Support Non-U.S. Gov't Weisser, Johan J Hansen, Martin Björklund, Erland Sonne, Christian Dietz, Rune Styrishave, Bjarne A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
topic_facet |
Journal Article Research Support Non-U.S. Gov't |
description |
This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)–solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC–MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to previous polar bear studies based on ELISA techniques. Absolute method recoveries were 81%, 75% and 60% for cortisol, corticosterone and aldosterone, respectively. We applied the developed method on a hair sample pooled from four East Greenland polar bears. Herein cortisol and corticosterone were successfully determined in levels of 0.32 ± 0.02 ng/g hair and 0.13 ± 0.02 ng/g hair, respectively. Aldosterone was below limit of detection (LOD < 0.17 ng/g). The cortisol hair concentration found in these East Greenland polar bears was consistent with cortisol levels previously determined in the Southern Hudson Bay and James Bay in Canada using ELISA kits. This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)-solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to ... |
format |
Article in Journal/Newspaper |
author |
Weisser, Johan J Hansen, Martin Björklund, Erland Sonne, Christian Dietz, Rune Styrishave, Bjarne |
author_facet |
Weisser, Johan J Hansen, Martin Björklund, Erland Sonne, Christian Dietz, Rune Styrishave, Bjarne |
author_sort |
Weisser, Johan J |
title |
A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
title_short |
A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
title_full |
A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
title_fullStr |
A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
title_full_unstemmed |
A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
title_sort |
novel method for analysing key corticosteroids in polar bear (ursus maritimus) hair using liquid chromatography tandem mass spectrometry |
publishDate |
2016 |
url |
https://curis.ku.dk/portal/da/publications/a-novel-method-for-analysing-key-corticosteroids-in-polar-bear-ursus-maritimus-hair-using-liquid-chromatography-tandem-mass-spectrometry(7250b45a-07b3-4bdd-b8f7-32600e50dd7f).html https://doi.org/10.1016/j.jchromb.2016.02.029 |
geographic |
Canada Greenland Hudson Hudson Bay |
geographic_facet |
Canada Greenland Hudson Hudson Bay |
genre |
East Greenland Greenland Hudson Bay Ursus maritimus James Bay |
genre_facet |
East Greenland Greenland Hudson Bay Ursus maritimus James Bay |
op_source |
Weisser , J J , Hansen , M , Björklund , E , Sonne , C , Dietz , R & Styrishave , B 2016 , ' A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry ' , Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 1017-1018 , pp. 45-51 . https://doi.org/10.1016/j.jchromb.2016.02.029 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.jchromb.2016.02.029 |
container_title |
Journal of Chromatography B |
container_volume |
1017-1018 |
container_start_page |
45 |
op_container_end_page |
51 |
_version_ |
1798845169787731968 |