Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels
Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synt...
Published in: | Environmental Pollution |
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Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2017
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Online Access: | https://curis.ku.dk/portal/da/publications/exposure-of-juvenile-turbot-scophthalmus-maximus-to-silver-nanoparticles-and-17ethinylestradiol-mixtures(6275337b-a068-44bc-87af-350808ff093c).html https://doi.org/10.1016/j.envpol.2016.09.067 |
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English |
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Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17α-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 μg L−1 and 200 μg L−1), Ag+ (50 μg L−1), EE2 (50 ng L−1) and AgNP + EE2 (2 or 200 μg L−1+50 ng L−1). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP + EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Ag+ exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17α-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 μg L(-1) and 200 μg L(-1)), Ag(+) (50 μg L(-1)), EE2 (50 ng L(-1)) and AgNP + EE2 (2 or 200 μg L(-1)+50 ng L(-1)). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP + EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Ag(+) exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. |
format |
Article in Journal/Newspaper |
author |
Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Staňková, Radka Ciesielski, Tomasz M Olsen, Anders J Posch, Wilfried Flaten, Trond P Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn M |
spellingShingle |
Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Staňková, Radka Ciesielski, Tomasz M Olsen, Anders J Posch, Wilfried Flaten, Trond P Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn M Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
author_facet |
Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Staňková, Radka Ciesielski, Tomasz M Olsen, Anders J Posch, Wilfried Flaten, Trond P Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn M |
author_sort |
Farkas, Julia |
title |
Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
title_short |
Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
title_full |
Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
title_fullStr |
Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
title_full_unstemmed |
Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels |
title_sort |
exposure of juvenile turbot (scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:implications for contaminant uptake and plasma steroid hormone levels |
publishDate |
2017 |
url |
https://curis.ku.dk/portal/da/publications/exposure-of-juvenile-turbot-scophthalmus-maximus-to-silver-nanoparticles-and-17ethinylestradiol-mixtures(6275337b-a068-44bc-87af-350808ff093c).html https://doi.org/10.1016/j.envpol.2016.09.067 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Farkas , J , Salaberria , I , Styrishave , B , Staňková , R , Ciesielski , T M , Olsen , A J , Posch , W , Flaten , T P , Krøkje , Å , Salvenmoser , W & Jenssen , B M 2017 , ' Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures : Implications for contaminant uptake and plasma steroid hormone levels ' , Environmental Pollution , vol. 220 , no. Part A , pp. 328-336 . https://doi.org/10.1016/j.envpol.2016.09.067 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.envpol.2016.09.067 |
container_title |
Environmental Pollution |
container_volume |
220 |
container_start_page |
328 |
op_container_end_page |
336 |
_version_ |
1766189024984170496 |
spelling |
ftcopenhagenunip:oai:pure.atira.dk:publications/6275337b-a068-44bc-87af-350808ff093c 2023-05-15T18:15:48+02:00 Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures:Implications for contaminant uptake and plasma steroid hormone levels Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Staňková, Radka Ciesielski, Tomasz M Olsen, Anders J Posch, Wilfried Flaten, Trond P Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn M 2017-01-01 https://curis.ku.dk/portal/da/publications/exposure-of-juvenile-turbot-scophthalmus-maximus-to-silver-nanoparticles-and-17ethinylestradiol-mixtures(6275337b-a068-44bc-87af-350808ff093c).html https://doi.org/10.1016/j.envpol.2016.09.067 eng eng info:eu-repo/semantics/closedAccess Farkas , J , Salaberria , I , Styrishave , B , Staňková , R , Ciesielski , T M , Olsen , A J , Posch , W , Flaten , T P , Krøkje , Å , Salvenmoser , W & Jenssen , B M 2017 , ' Exposure of juvenile turbot (Scophthalmus maximus) to silver nanoparticles and 17α-ethinylestradiol mixtures : Implications for contaminant uptake and plasma steroid hormone levels ' , Environmental Pollution , vol. 220 , no. Part A , pp. 328-336 . https://doi.org/10.1016/j.envpol.2016.09.067 article 2017 ftcopenhagenunip https://doi.org/10.1016/j.envpol.2016.09.067 2022-02-24T00:23:28Z Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17α-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 μg L−1 and 200 μg L−1), Ag+ (50 μg L−1), EE2 (50 ng L−1) and AgNP + EE2 (2 or 200 μg L−1+50 ng L−1). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP + EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Ag+ exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17α-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 μg L(-1) and 200 μg L(-1)), Ag(+) (50 μg L(-1)), EE2 (50 ng L(-1)) and AgNP + EE2 (2 or 200 μg L(-1)+50 ng L(-1)). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP + EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Ag(+) exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. Article in Journal/Newspaper Scophthalmus maximus Turbot University of Copenhagen: Research Environmental Pollution 220 328 336 |