The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men
Background: The organochlorine dichlorodiphenyltrichloroethane (DDT) is banned worldwide owing to its negative health effects. It is exceptionally used as an insecticide for malaria control. Exposure occurs in regions where DDT is applied, as well as in the Arctic, where its endocrine disrupting met...
Published in: | Environmental Health Perspectives |
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2024
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Online Access: | https://curis.ku.dk/portal/da/publications/the-association-between-longterm-ddt-or-dde-exposures-and-an-altered-sperm-epigenomea-crosssectional-study-of-greenlandic-inuit-and-south-african-vhavenda-men(588e9d34-ce12-412c-beac-8cceea6bcaa8).html https://doi.org/10.1289/EHP12013 https://curis.ku.dk/ws/files/388323388/lismer_et_al_2024_the_association_between_long_term_ddt_or_dde_exposures_and_an_altered_sperm_epigenome_a_cross.pdf |
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ftcopenhagenunip:oai:pure.atira.dk:publications/588e9d34-ce12-412c-beac-8cceea6bcaa8 2024-06-09T07:44:26+00:00 The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men Lismer, Ariane Shao, Xiaojian Dumargne, Marie-Charlotte Lafleur, Christine Lambrot, Romain Chan, Donovan Toft, Gunnar Bonde, Jens Peter MacFarlane, Amanda J Bornman, Riana Aneck-Hahn, Natalie Patrick, Sean Bailey, Janice M de Jager, Christiaan Dumeaux, Vanessa Trasler, Jacquetta M Kimmins, Sarah 2024 application/pdf https://curis.ku.dk/portal/da/publications/the-association-between-longterm-ddt-or-dde-exposures-and-an-altered-sperm-epigenomea-crosssectional-study-of-greenlandic-inuit-and-south-african-vhavenda-men(588e9d34-ce12-412c-beac-8cceea6bcaa8).html https://doi.org/10.1289/EHP12013 https://curis.ku.dk/ws/files/388323388/lismer_et_al_2024_the_association_between_long_term_ddt_or_dde_exposures_and_an_altered_sperm_epigenome_a_cross.pdf eng eng info:eu-repo/semantics/openAccess Lismer , A , Shao , X , Dumargne , M-C , Lafleur , C , Lambrot , R , Chan , D , Toft , G , Bonde , J P , MacFarlane , A J , Bornman , R , Aneck-Hahn , N , Patrick , S , Bailey , J M , de Jager , C , Dumeaux , V , Trasler , J M & Kimmins , S 2024 , ' The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men ' , Environmental Health Perspectives , vol. 132 , no. 1 , 17008 . https://doi.org/10.1289/EHP12013 Humans Male Cross-Sectional Studies DDT/toxicity Dichlorodiphenyl Dichloroethylene/toxicity Epigenome Inuit Semen South Africa/epidemiology Spermatozoa Black People article 2024 ftcopenhagenunip https://doi.org/10.1289/EHP12013 2024-05-16T11:29:31Z Background: The organochlorine dichlorodiphenyltrichloroethane (DDT) is banned worldwide owing to its negative health effects. It is exceptionally used as an insecticide for malaria control. Exposure occurs in regions where DDT is applied, as well as in the Arctic, where its endocrine disrupting metabolite, -dichlorodiphenyldichloroethylene ( -DDE) accumulates in marine mammals and fish. DDT and -DDE exposures are linked to birth defects, infertility, cancer, and neurodevelopmental delays. Of particular concern is the potential of DDT use to impact the health of generations to come via the heritable sperm epigenome. Objectives: The objective of this study was to assess the sperm epigenome in relation to -DDE serum levels between geographically diverse populations. Methods: In the Limpopo Province of South Africa, we recruited 247 VhaVenda South African men and selected 50 paired blood serum and semen samples, and 47 Greenlandic Inuit blood and semen paired samples were selected from a total of 193 samples from the biobank of the INUENDO cohort, an EU Fifth Framework Programme Research and Development project. Sample selection was based on obtaining a range of -DDE serum levels ( ). We assessed the sperm epigenome in relation to serum -DDE levels using MethylC-Capture-sequencing (MCC-seq) and chromatin immunoprecipitation followed by sequencing (ChIP-seq). We identified genomic regions with altered DNA methylation (DNAme) and differential enrichment of histone H3 lysine 4 trimethylation (H3K4me3) in sperm. Results: Differences in DNAme and H3K4me3 enrichment were identified at transposable elements and regulatory regions involved in fertility, disease, development, and neurofunction. A subset of regions with sperm DNAme and H3K4me3 that differed between exposure groups was predicted to persist in the preimplantation embryo and to be associated with embryonic gene expression. Discussion: These findings suggest that DDT and -DDE exposure impacts the sperm epigenome in a dose–response-like manner and may negatively ... Article in Journal/Newspaper Arctic greenlandic inuit University of Copenhagen: Research Arctic Environmental Health Perspectives 132 1 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Humans Male Cross-Sectional Studies DDT/toxicity Dichlorodiphenyl Dichloroethylene/toxicity Epigenome Inuit Semen South Africa/epidemiology Spermatozoa Black People |
spellingShingle |
Humans Male Cross-Sectional Studies DDT/toxicity Dichlorodiphenyl Dichloroethylene/toxicity Epigenome Inuit Semen South Africa/epidemiology Spermatozoa Black People Lismer, Ariane Shao, Xiaojian Dumargne, Marie-Charlotte Lafleur, Christine Lambrot, Romain Chan, Donovan Toft, Gunnar Bonde, Jens Peter MacFarlane, Amanda J Bornman, Riana Aneck-Hahn, Natalie Patrick, Sean Bailey, Janice M de Jager, Christiaan Dumeaux, Vanessa Trasler, Jacquetta M Kimmins, Sarah The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
topic_facet |
Humans Male Cross-Sectional Studies DDT/toxicity Dichlorodiphenyl Dichloroethylene/toxicity Epigenome Inuit Semen South Africa/epidemiology Spermatozoa Black People |
description |
Background: The organochlorine dichlorodiphenyltrichloroethane (DDT) is banned worldwide owing to its negative health effects. It is exceptionally used as an insecticide for malaria control. Exposure occurs in regions where DDT is applied, as well as in the Arctic, where its endocrine disrupting metabolite, -dichlorodiphenyldichloroethylene ( -DDE) accumulates in marine mammals and fish. DDT and -DDE exposures are linked to birth defects, infertility, cancer, and neurodevelopmental delays. Of particular concern is the potential of DDT use to impact the health of generations to come via the heritable sperm epigenome. Objectives: The objective of this study was to assess the sperm epigenome in relation to -DDE serum levels between geographically diverse populations. Methods: In the Limpopo Province of South Africa, we recruited 247 VhaVenda South African men and selected 50 paired blood serum and semen samples, and 47 Greenlandic Inuit blood and semen paired samples were selected from a total of 193 samples from the biobank of the INUENDO cohort, an EU Fifth Framework Programme Research and Development project. Sample selection was based on obtaining a range of -DDE serum levels ( ). We assessed the sperm epigenome in relation to serum -DDE levels using MethylC-Capture-sequencing (MCC-seq) and chromatin immunoprecipitation followed by sequencing (ChIP-seq). We identified genomic regions with altered DNA methylation (DNAme) and differential enrichment of histone H3 lysine 4 trimethylation (H3K4me3) in sperm. Results: Differences in DNAme and H3K4me3 enrichment were identified at transposable elements and regulatory regions involved in fertility, disease, development, and neurofunction. A subset of regions with sperm DNAme and H3K4me3 that differed between exposure groups was predicted to persist in the preimplantation embryo and to be associated with embryonic gene expression. Discussion: These findings suggest that DDT and -DDE exposure impacts the sperm epigenome in a dose–response-like manner and may negatively ... |
format |
Article in Journal/Newspaper |
author |
Lismer, Ariane Shao, Xiaojian Dumargne, Marie-Charlotte Lafleur, Christine Lambrot, Romain Chan, Donovan Toft, Gunnar Bonde, Jens Peter MacFarlane, Amanda J Bornman, Riana Aneck-Hahn, Natalie Patrick, Sean Bailey, Janice M de Jager, Christiaan Dumeaux, Vanessa Trasler, Jacquetta M Kimmins, Sarah |
author_facet |
Lismer, Ariane Shao, Xiaojian Dumargne, Marie-Charlotte Lafleur, Christine Lambrot, Romain Chan, Donovan Toft, Gunnar Bonde, Jens Peter MacFarlane, Amanda J Bornman, Riana Aneck-Hahn, Natalie Patrick, Sean Bailey, Janice M de Jager, Christiaan Dumeaux, Vanessa Trasler, Jacquetta M Kimmins, Sarah |
author_sort |
Lismer, Ariane |
title |
The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
title_short |
The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
title_full |
The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
title_fullStr |
The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
title_full_unstemmed |
The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men |
title_sort |
association between long-term ddt or dde exposures and an altered sperm epigenome-a cross-sectional study of greenlandic inuit and south african vhavenda men |
publishDate |
2024 |
url |
https://curis.ku.dk/portal/da/publications/the-association-between-longterm-ddt-or-dde-exposures-and-an-altered-sperm-epigenomea-crosssectional-study-of-greenlandic-inuit-and-south-african-vhavenda-men(588e9d34-ce12-412c-beac-8cceea6bcaa8).html https://doi.org/10.1289/EHP12013 https://curis.ku.dk/ws/files/388323388/lismer_et_al_2024_the_association_between_long_term_ddt_or_dde_exposures_and_an_altered_sperm_epigenome_a_cross.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic greenlandic inuit |
genre_facet |
Arctic greenlandic inuit |
op_source |
Lismer , A , Shao , X , Dumargne , M-C , Lafleur , C , Lambrot , R , Chan , D , Toft , G , Bonde , J P , MacFarlane , A J , Bornman , R , Aneck-Hahn , N , Patrick , S , Bailey , J M , de Jager , C , Dumeaux , V , Trasler , J M & Kimmins , S 2024 , ' The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men ' , Environmental Health Perspectives , vol. 132 , no. 1 , 17008 . https://doi.org/10.1289/EHP12013 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1289/EHP12013 |
container_title |
Environmental Health Perspectives |
container_volume |
132 |
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1 |
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1801373176515526656 |