Isolation of nucleic acids and cultures from fossil ice and permafrost

Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and micr...

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Published in:Trends in Ecology & Evolution
Main Authors: Willerslev, E., Hansen, Anders J., Poinar, H. N.
Format: Article in Journal/Newspaper
Language:English
Published: 2004
Subjects:
Ice
permafrost
Online Access:https://curis.ku.dk/portal/da/publications/isolation-of-nucleic-acids-and-cultures-from-fossil-ice-and-permafrost(4b18b2f0-98c6-11de-bc73-000ea68e967b).html
https://doi.org/10.1016/j.tree.2003.11.010
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spelling ftcopenhagenunip:oai:pure.atira.dk:publications/4b18b2f0-98c6-11de-bc73-000ea68e967b 2023-05-15T16:36:38+02:00 Isolation of nucleic acids and cultures from fossil ice and permafrost Willerslev, E. Hansen, Anders J. Poinar, H. N. 2004 https://curis.ku.dk/portal/da/publications/isolation-of-nucleic-acids-and-cultures-from-fossil-ice-and-permafrost(4b18b2f0-98c6-11de-bc73-000ea68e967b).html https://doi.org/10.1016/j.tree.2003.11.010 eng eng info:eu-repo/semantics/restrictedAccess Willerslev , E , Hansen , A J & Poinar , H N 2004 , ' Isolation of nucleic acids and cultures from fossil ice and permafrost ' , Trends in Ecology & Evolution , vol. 19 , no. 3 , pp. 2004 Mar;19(3):141-7 . https://doi.org/10.1016/j.tree.2003.11.010 article 2004 ftcopenhagenunip https://doi.org/10.1016/j.tree.2003.11.010 2022-02-24T00:02:32Z Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and microbial nucleic acids obtained from ice- and permafrost cores from hundreds of thousands to millions of years old are not properly authenticated and the findings could be the result of contamination. Here, we discuss the processes that restrict the long-term survival of DNA and/or RNA molecules in ice and permafrost, and highlight sources of contamination that could result in false claims. Additionally, we present a set of precautions, controls and criteria to help ensure that future cultures and sequences are authentic. Udgivelsesdato: 2004 Mar Article in Journal/Newspaper Ice permafrost University of Copenhagen: Research Trends in Ecology & Evolution 19 3 141 147
institution Open Polar
collection University of Copenhagen: Research
op_collection_id ftcopenhagenunip
language English
description Owing to their constant low temperatures, glacial ice and permafrost might contain the oldest nucleic acids and microbial cells on Earth, which could prove key to reconstructing past ecosystems and for the planning of missions to other planets. However, recent claims concerning viable cells and microbial nucleic acids obtained from ice- and permafrost cores from hundreds of thousands to millions of years old are not properly authenticated and the findings could be the result of contamination. Here, we discuss the processes that restrict the long-term survival of DNA and/or RNA molecules in ice and permafrost, and highlight sources of contamination that could result in false claims. Additionally, we present a set of precautions, controls and criteria to help ensure that future cultures and sequences are authentic. Udgivelsesdato: 2004 Mar
format Article in Journal/Newspaper
author Willerslev, E.
Hansen, Anders J.
Poinar, H. N.
spellingShingle Willerslev, E.
Hansen, Anders J.
Poinar, H. N.
Isolation of nucleic acids and cultures from fossil ice and permafrost
author_facet Willerslev, E.
Hansen, Anders J.
Poinar, H. N.
author_sort Willerslev, E.
title Isolation of nucleic acids and cultures from fossil ice and permafrost
title_short Isolation of nucleic acids and cultures from fossil ice and permafrost
title_full Isolation of nucleic acids and cultures from fossil ice and permafrost
title_fullStr Isolation of nucleic acids and cultures from fossil ice and permafrost
title_full_unstemmed Isolation of nucleic acids and cultures from fossil ice and permafrost
title_sort isolation of nucleic acids and cultures from fossil ice and permafrost
publishDate 2004
url https://curis.ku.dk/portal/da/publications/isolation-of-nucleic-acids-and-cultures-from-fossil-ice-and-permafrost(4b18b2f0-98c6-11de-bc73-000ea68e967b).html
https://doi.org/10.1016/j.tree.2003.11.010
genre Ice
permafrost
genre_facet Ice
permafrost
op_source Willerslev , E , Hansen , A J & Poinar , H N 2004 , ' Isolation of nucleic acids and cultures from fossil ice and permafrost ' , Trends in Ecology & Evolution , vol. 19 , no. 3 , pp. 2004 Mar;19(3):141-7 . https://doi.org/10.1016/j.tree.2003.11.010
op_rights info:eu-repo/semantics/restrictedAccess
op_doi https://doi.org/10.1016/j.tree.2003.11.010
container_title Trends in Ecology & Evolution
container_volume 19
container_issue 3
container_start_page 141
op_container_end_page 147
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