Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes
BACKGROUND: There has been interest in determining whether older red blood cell (RBC) units have negative clinical effects. Numerous observational studies have shown that older RBC units are an independent factor for patient mortality. However, recently published randomized clinical trials have show...
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Online Access: | https://curis.ku.dk/portal/da/publications/identified-metabolic-signature-for-assessing-red-blood-cell-unit-quality-is-associated-with-endothelial-damage-markers-and-clinical-outcomes(49dd7f20-d290-44cc-94e4-f53dd26dc4e1).html https://doi.org/10.1111/trf.13460 |
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ftcopenhagenunip:oai:pure.atira.dk:publications/49dd7f20-d290-44cc-94e4-f53dd26dc4e1 2023-12-10T09:50:02+01:00 Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes Bordbar, Aarash Johansson, Pär I Paglia, Giuseppe Harrison, Scott J Wichuk, Kristine Magnusdottir, Manuela Valgeirsdottir, Sóley Gybel-Brask, Mikkel Ostrowski, Sisse R Palsson, Sirus Rolfsson, Ottar Sigurjónsson, Olafur E Hansen, Morten B Gudmundsson, Sveinn Palsson, Bernhard O 2016-04 https://curis.ku.dk/portal/da/publications/identified-metabolic-signature-for-assessing-red-blood-cell-unit-quality-is-associated-with-endothelial-damage-markers-and-clinical-outcomes(49dd7f20-d290-44cc-94e4-f53dd26dc4e1).html https://doi.org/10.1111/trf.13460 eng eng info:eu-repo/semantics/closedAccess Bordbar , A , Johansson , P I , Paglia , G , Harrison , S J , Wichuk , K , Magnusdottir , M , Valgeirsdottir , S , Gybel-Brask , M , Ostrowski , S R , Palsson , S , Rolfsson , O , Sigurjónsson , O E , Hansen , M B , Gudmundsson , S & Palsson , B O 2016 , ' Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes ' , Transfusion , vol. 56 , no. 4 , pp. 852-62 . https://doi.org/10.1111/trf.13460 Biomarkers Blood Preservation Denmark Endothelium Vascular Erythrocyte Transfusion Erythrocytes Healthy Volunteers Humans Iceland Male Metabolome Metabolomics Quality Control Treatment Outcome Journal Article Observational Study Research Support N.I.H. Extramural Non-U.S. Gov't article 2016 ftcopenhagenunip https://doi.org/10.1111/trf.13460 2023-11-16T00:00:09Z BACKGROUND: There has been interest in determining whether older red blood cell (RBC) units have negative clinical effects. Numerous observational studies have shown that older RBC units are an independent factor for patient mortality. However, recently published randomized clinical trials have shown no difference of clinical outcome for patients receiving old or fresh RBCs. An overlooked but essential issue in assessing RBC unit quality and ultimately designing the necessary clinical trials is a metric for what constitutes an old or fresh RBC unit. STUDY DESIGN AND METHODS: Twenty RBC units were profiled using quantitative metabolomics over 42 days of storage in SAGM with 3- to 4-day time intervals. Metabolic pathway usage during storage was assessed using systems biology methods. The detected time intervals of the metabolic states were compared to clinical outcomes. RESULTS: Using multivariate statistics, we identified a nonlinear decay process exhibiting three distinct metabolic states (Days 0-10, 10-17, and 17-42). Hematologic variables traditionally measured in the transfusion setting (e.g., pH, hemolysis, RBC indices) did not distinguish these three states. Systemic changes in pathway usage occurred between the three states, with key pathways changing in both magnitude and direction. Finally, an association was found between the time periods of the metabolic states with the clinical outcomes of more than 280,000 patients in the country of Denmark transfused over the past 15 years and endothelial damage markers in healthy volunteers undergoing autologous transfusions. CONCLUSION: The state of RBC metabolism may be a better indicator of cellular quality than traditional hematologic variables. Article in Journal/Newspaper Iceland University of Copenhagen: Research Transfusion 56 4 852 862 |
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University of Copenhagen: Research |
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ftcopenhagenunip |
language |
English |
topic |
Biomarkers Blood Preservation Denmark Endothelium Vascular Erythrocyte Transfusion Erythrocytes Healthy Volunteers Humans Iceland Male Metabolome Metabolomics Quality Control Treatment Outcome Journal Article Observational Study Research Support N.I.H. Extramural Non-U.S. Gov't |
spellingShingle |
Biomarkers Blood Preservation Denmark Endothelium Vascular Erythrocyte Transfusion Erythrocytes Healthy Volunteers Humans Iceland Male Metabolome Metabolomics Quality Control Treatment Outcome Journal Article Observational Study Research Support N.I.H. Extramural Non-U.S. Gov't Bordbar, Aarash Johansson, Pär I Paglia, Giuseppe Harrison, Scott J Wichuk, Kristine Magnusdottir, Manuela Valgeirsdottir, Sóley Gybel-Brask, Mikkel Ostrowski, Sisse R Palsson, Sirus Rolfsson, Ottar Sigurjónsson, Olafur E Hansen, Morten B Gudmundsson, Sveinn Palsson, Bernhard O Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
topic_facet |
Biomarkers Blood Preservation Denmark Endothelium Vascular Erythrocyte Transfusion Erythrocytes Healthy Volunteers Humans Iceland Male Metabolome Metabolomics Quality Control Treatment Outcome Journal Article Observational Study Research Support N.I.H. Extramural Non-U.S. Gov't |
description |
BACKGROUND: There has been interest in determining whether older red blood cell (RBC) units have negative clinical effects. Numerous observational studies have shown that older RBC units are an independent factor for patient mortality. However, recently published randomized clinical trials have shown no difference of clinical outcome for patients receiving old or fresh RBCs. An overlooked but essential issue in assessing RBC unit quality and ultimately designing the necessary clinical trials is a metric for what constitutes an old or fresh RBC unit. STUDY DESIGN AND METHODS: Twenty RBC units were profiled using quantitative metabolomics over 42 days of storage in SAGM with 3- to 4-day time intervals. Metabolic pathway usage during storage was assessed using systems biology methods. The detected time intervals of the metabolic states were compared to clinical outcomes. RESULTS: Using multivariate statistics, we identified a nonlinear decay process exhibiting three distinct metabolic states (Days 0-10, 10-17, and 17-42). Hematologic variables traditionally measured in the transfusion setting (e.g., pH, hemolysis, RBC indices) did not distinguish these three states. Systemic changes in pathway usage occurred between the three states, with key pathways changing in both magnitude and direction. Finally, an association was found between the time periods of the metabolic states with the clinical outcomes of more than 280,000 patients in the country of Denmark transfused over the past 15 years and endothelial damage markers in healthy volunteers undergoing autologous transfusions. CONCLUSION: The state of RBC metabolism may be a better indicator of cellular quality than traditional hematologic variables. |
format |
Article in Journal/Newspaper |
author |
Bordbar, Aarash Johansson, Pär I Paglia, Giuseppe Harrison, Scott J Wichuk, Kristine Magnusdottir, Manuela Valgeirsdottir, Sóley Gybel-Brask, Mikkel Ostrowski, Sisse R Palsson, Sirus Rolfsson, Ottar Sigurjónsson, Olafur E Hansen, Morten B Gudmundsson, Sveinn Palsson, Bernhard O |
author_facet |
Bordbar, Aarash Johansson, Pär I Paglia, Giuseppe Harrison, Scott J Wichuk, Kristine Magnusdottir, Manuela Valgeirsdottir, Sóley Gybel-Brask, Mikkel Ostrowski, Sisse R Palsson, Sirus Rolfsson, Ottar Sigurjónsson, Olafur E Hansen, Morten B Gudmundsson, Sveinn Palsson, Bernhard O |
author_sort |
Bordbar, Aarash |
title |
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
title_short |
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
title_full |
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
title_fullStr |
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
title_full_unstemmed |
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
title_sort |
identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes |
publishDate |
2016 |
url |
https://curis.ku.dk/portal/da/publications/identified-metabolic-signature-for-assessing-red-blood-cell-unit-quality-is-associated-with-endothelial-damage-markers-and-clinical-outcomes(49dd7f20-d290-44cc-94e4-f53dd26dc4e1).html https://doi.org/10.1111/trf.13460 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Bordbar , A , Johansson , P I , Paglia , G , Harrison , S J , Wichuk , K , Magnusdottir , M , Valgeirsdottir , S , Gybel-Brask , M , Ostrowski , S R , Palsson , S , Rolfsson , O , Sigurjónsson , O E , Hansen , M B , Gudmundsson , S & Palsson , B O 2016 , ' Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes ' , Transfusion , vol. 56 , no. 4 , pp. 852-62 . https://doi.org/10.1111/trf.13460 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/trf.13460 |
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Transfusion |
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