Establishing a community-wide DNA barcode library as a new tool for arctic research
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area...
Published in: | Molecular Ecology Resources |
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2016
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Online Access: | https://curis.ku.dk/portal/da/publications/establishing-a-communitywide-dna-barcode-library-as-a-new-tool-for-arctic-research(3bbe97b1-80e7-4d66-ab7d-ffc13b228041).html https://doi.org/10.1111/1755-0998.12489 http://www.scopus.com/inward/record.url?scp=84950327054&partnerID=8YFLogxK |
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ftcopenhagenunip:oai:pure.atira.dk:publications/3bbe97b1-80e7-4d66-ab7d-ffc13b228041 2024-05-12T07:57:41+00:00 Establishing a community-wide DNA barcode library as a new tool for arctic research Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, Peter Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, Verner Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. 2016 https://curis.ku.dk/portal/da/publications/establishing-a-communitywide-dna-barcode-library-as-a-new-tool-for-arctic-research(3bbe97b1-80e7-4d66-ab7d-ffc13b228041).html https://doi.org/10.1111/1755-0998.12489 http://www.scopus.com/inward/record.url?scp=84950327054&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Wirta , H , Várkonyi , G , Rasmussen , C , Kaartinen , R , Schmidt , N M , Hebert , P D N , Barták , M , Blagoev , G , Disney , H , Ertl , S , Gjelstrup , P , Gwiazdowicz , D J , Huldén , L , Ilmonen , J , Jakovlev , J , Jaschhof , M , Kahanpää , J , Kankaanpää , T , Krogh , P H , Labbee , R , Lettner , C , Michelsen , V , Nielsen , S A , Nielsen , T R , Paasivirta , L , Pedersen , S , Pohjoismäki , J , Salmela , J , Vilkamaa , P , Väre , H , von Tschirnhaus , M & Roslin , T 2016 , ' Establishing a community-wide DNA barcode library as a new tool for arctic research ' , Molecular Ecology Resources , vol. 16 , no. 3 , pp. 809-822 . https://doi.org/10.1111/1755-0998.12489 Arthropod DNA barcode library Greenland High arctic Species diversity article 2016 ftcopenhagenunip https://doi.org/10.1111/1755-0998.12489 2024-04-18T00:28:13Z DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. Article in Journal/Newspaper Arctic Arctic Greenland Zackenberg University of Copenhagen: Research Arctic Greenland Molecular Ecology Resources 16 3 809 822 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
Arthropod DNA barcode library Greenland High arctic Species diversity |
spellingShingle |
Arthropod DNA barcode library Greenland High arctic Species diversity Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, Peter Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, Verner Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. Establishing a community-wide DNA barcode library as a new tool for arctic research |
topic_facet |
Arthropod DNA barcode library Greenland High arctic Species diversity |
description |
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. |
format |
Article in Journal/Newspaper |
author |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, Peter Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, Verner Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_facet |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, Peter Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, Verner Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. von Tschirnhaus, M. Roslin, T. |
author_sort |
Wirta, H. |
title |
Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_short |
Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full |
Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_fullStr |
Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full_unstemmed |
Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_sort |
establishing a community-wide dna barcode library as a new tool for arctic research |
publishDate |
2016 |
url |
https://curis.ku.dk/portal/da/publications/establishing-a-communitywide-dna-barcode-library-as-a-new-tool-for-arctic-research(3bbe97b1-80e7-4d66-ab7d-ffc13b228041).html https://doi.org/10.1111/1755-0998.12489 http://www.scopus.com/inward/record.url?scp=84950327054&partnerID=8YFLogxK |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic Greenland Zackenberg |
genre_facet |
Arctic Arctic Greenland Zackenberg |
op_source |
Wirta , H , Várkonyi , G , Rasmussen , C , Kaartinen , R , Schmidt , N M , Hebert , P D N , Barták , M , Blagoev , G , Disney , H , Ertl , S , Gjelstrup , P , Gwiazdowicz , D J , Huldén , L , Ilmonen , J , Jakovlev , J , Jaschhof , M , Kahanpää , J , Kankaanpää , T , Krogh , P H , Labbee , R , Lettner , C , Michelsen , V , Nielsen , S A , Nielsen , T R , Paasivirta , L , Pedersen , S , Pohjoismäki , J , Salmela , J , Vilkamaa , P , Väre , H , von Tschirnhaus , M & Roslin , T 2016 , ' Establishing a community-wide DNA barcode library as a new tool for arctic research ' , Molecular Ecology Resources , vol. 16 , no. 3 , pp. 809-822 . https://doi.org/10.1111/1755-0998.12489 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/1755-0998.12489 |
container_title |
Molecular Ecology Resources |
container_volume |
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822 |
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