A multiplex assay with 52 single nucleotide polymorphisms for human identification
Udgivelsesdato: 2006-May A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commo...
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ftcopenhagenunip:oai:pure.atira.dk:publications/31e940c0-97ad-11de-8bc9-000ea68e967b 2023-12-24T10:17:12+01:00 A multiplex assay with 52 single nucleotide polymorphisms for human identification Sanchez Sanchez, Juan Jose Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe-Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels 2006 https://curis.ku.dk/portal/da/publications/a-multiplex-assay-with-52-single-nucleotide-polymorphisms-for-human-identification(31e940c0-97ad-11de-8bc9-000ea68e967b).html https://doi.org/10.1002/elps.200500671 eng eng info:eu-repo/semantics/restrictedAccess Sanchez Sanchez , J J , Phillips , C , Børsting , C , Balogh , K , Bogus , M , Fondevila , M , Harrison , C D , Musgrave-Brown , E , Salas , A , Syndercombe-Court , D , Schneider , P M , Carracedo , A & Morling , N 2006 , ' A multiplex assay with 52 single nucleotide polymorphisms for human identification ' , Electrophoresis , vol. 27 , no. 9 , pp. 1713-24 . https://doi.org/10.1002/elps.200500671 article 2006 ftcopenhagenunip https://doi.org/10.1002/elps.200500671 2023-11-29T23:59:11Z Udgivelsesdato: 2006-May A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org. Article in Journal/Newspaper Greenland University of Copenhagen: Research Greenland ELECTROPHORESIS 27 9 1713 1724 |
institution |
Open Polar |
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University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
description |
Udgivelsesdato: 2006-May A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org. |
format |
Article in Journal/Newspaper |
author |
Sanchez Sanchez, Juan Jose Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe-Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels |
spellingShingle |
Sanchez Sanchez, Juan Jose Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe-Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels A multiplex assay with 52 single nucleotide polymorphisms for human identification |
author_facet |
Sanchez Sanchez, Juan Jose Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe-Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels |
author_sort |
Sanchez Sanchez, Juan Jose |
title |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_short |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_full |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_fullStr |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_full_unstemmed |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_sort |
multiplex assay with 52 single nucleotide polymorphisms for human identification |
publishDate |
2006 |
url |
https://curis.ku.dk/portal/da/publications/a-multiplex-assay-with-52-single-nucleotide-polymorphisms-for-human-identification(31e940c0-97ad-11de-8bc9-000ea68e967b).html https://doi.org/10.1002/elps.200500671 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Sanchez Sanchez , J J , Phillips , C , Børsting , C , Balogh , K , Bogus , M , Fondevila , M , Harrison , C D , Musgrave-Brown , E , Salas , A , Syndercombe-Court , D , Schneider , P M , Carracedo , A & Morling , N 2006 , ' A multiplex assay with 52 single nucleotide polymorphisms for human identification ' , Electrophoresis , vol. 27 , no. 9 , pp. 1713-24 . https://doi.org/10.1002/elps.200500671 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1002/elps.200500671 |
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ELECTROPHORESIS |
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27 |
container_issue |
9 |
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1713 |
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1724 |
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1786205177137594368 |