Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds
Microbes active in extreme cold are not as well explored as those of other extreme environments. Studies have revealed a substantial microbial diversity and identified cold-specific microbiome molecular functions. We analyzed the metagenomes and metatranscriptomes of 20 snow samples collected in ear...
Published in: | MicrobiologyOpen |
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2020
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Online Access: | https://curis.ku.dk/portal/da/publications/snow-microbiome-functional-analyses-reveal-novel-aspects-of-microbial-metabolism-of-complex-organic-compounds(2e1b2587-c7dc-4b43-b422-48ba9ff84285).html https://doi.org/10.1002/mbo3.1100 https://curis.ku.dk/ws/files/270335200/mbo3.1100.pdf |
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ftcopenhagenunip:oai:pure.atira.dk:publications/2e1b2587-c7dc-4b43-b422-48ba9ff84285 2024-04-28T08:40:09+00:00 Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds Zhu, Chengsheng Miller, Maximilian Lusskin, Nicholas Bergk Pinto, Benoît Maccario, Lorrie Häggblom, Max Vogel, Timothy Larose, Catherine Bromberg, Yana 2020 application/pdf https://curis.ku.dk/portal/da/publications/snow-microbiome-functional-analyses-reveal-novel-aspects-of-microbial-metabolism-of-complex-organic-compounds(2e1b2587-c7dc-4b43-b422-48ba9ff84285).html https://doi.org/10.1002/mbo3.1100 https://curis.ku.dk/ws/files/270335200/mbo3.1100.pdf eng eng info:eu-repo/semantics/openAccess Zhu , C , Miller , M , Lusskin , N , Bergk Pinto , B , Maccario , L , Häggblom , M , Vogel , T , Larose , C & Bromberg , Y 2020 , ' Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds ' , MicrobiologyOpen , vol. 9 , no. 9 , e1100 . https://doi.org/10.1002/mbo3.1100 metagenome metatranscriptome mi-faser snow microbiome article 2020 ftcopenhagenunip https://doi.org/10.1002/mbo3.1100 2024-04-04T17:35:16Z Microbes active in extreme cold are not as well explored as those of other extreme environments. Studies have revealed a substantial microbial diversity and identified cold-specific microbiome molecular functions. We analyzed the metagenomes and metatranscriptomes of 20 snow samples collected in early and late spring in Svalbard, Norway using mi-faser, our read-based computational microbiome function annotation tool. Our results reveal a more diverse microbiome functional capacity and activity in the early- vs. late-spring samples. We also find that functional dissimilarity between the same-sample metagenomes and metatranscriptomes is significantly higher in early than late spring samples. These findings suggest that early spring samples may contain a larger fraction of DNA of dormant (or dead) organisms, while late spring samples reflect a new, metabolically active community. We further show that the abundance of sequencing reads mapping to the fatty acid synthesis-related microbial pathways in late spring metagenomes and metatranscriptomes is significantly correlated with the organic acid levels measured in these samples. Similarly, the organic acid levels correlate with the pathway read abundances of geraniol degradation and inversely correlate with those of styrene degradation, suggesting a possible nutrient change. Our study thus highlights the activity of microbial degradation pathways of complex organic compounds previously unreported at low temperatures. Article in Journal/Newspaper Svalbard University of Copenhagen: Research MicrobiologyOpen 9 9 |
institution |
Open Polar |
collection |
University of Copenhagen: Research |
op_collection_id |
ftcopenhagenunip |
language |
English |
topic |
metagenome metatranscriptome mi-faser snow microbiome |
spellingShingle |
metagenome metatranscriptome mi-faser snow microbiome Zhu, Chengsheng Miller, Maximilian Lusskin, Nicholas Bergk Pinto, Benoît Maccario, Lorrie Häggblom, Max Vogel, Timothy Larose, Catherine Bromberg, Yana Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
topic_facet |
metagenome metatranscriptome mi-faser snow microbiome |
description |
Microbes active in extreme cold are not as well explored as those of other extreme environments. Studies have revealed a substantial microbial diversity and identified cold-specific microbiome molecular functions. We analyzed the metagenomes and metatranscriptomes of 20 snow samples collected in early and late spring in Svalbard, Norway using mi-faser, our read-based computational microbiome function annotation tool. Our results reveal a more diverse microbiome functional capacity and activity in the early- vs. late-spring samples. We also find that functional dissimilarity between the same-sample metagenomes and metatranscriptomes is significantly higher in early than late spring samples. These findings suggest that early spring samples may contain a larger fraction of DNA of dormant (or dead) organisms, while late spring samples reflect a new, metabolically active community. We further show that the abundance of sequencing reads mapping to the fatty acid synthesis-related microbial pathways in late spring metagenomes and metatranscriptomes is significantly correlated with the organic acid levels measured in these samples. Similarly, the organic acid levels correlate with the pathway read abundances of geraniol degradation and inversely correlate with those of styrene degradation, suggesting a possible nutrient change. Our study thus highlights the activity of microbial degradation pathways of complex organic compounds previously unreported at low temperatures. |
format |
Article in Journal/Newspaper |
author |
Zhu, Chengsheng Miller, Maximilian Lusskin, Nicholas Bergk Pinto, Benoît Maccario, Lorrie Häggblom, Max Vogel, Timothy Larose, Catherine Bromberg, Yana |
author_facet |
Zhu, Chengsheng Miller, Maximilian Lusskin, Nicholas Bergk Pinto, Benoît Maccario, Lorrie Häggblom, Max Vogel, Timothy Larose, Catherine Bromberg, Yana |
author_sort |
Zhu, Chengsheng |
title |
Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
title_short |
Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
title_full |
Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
title_fullStr |
Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
title_full_unstemmed |
Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
title_sort |
snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds |
publishDate |
2020 |
url |
https://curis.ku.dk/portal/da/publications/snow-microbiome-functional-analyses-reveal-novel-aspects-of-microbial-metabolism-of-complex-organic-compounds(2e1b2587-c7dc-4b43-b422-48ba9ff84285).html https://doi.org/10.1002/mbo3.1100 https://curis.ku.dk/ws/files/270335200/mbo3.1100.pdf |
genre |
Svalbard |
genre_facet |
Svalbard |
op_source |
Zhu , C , Miller , M , Lusskin , N , Bergk Pinto , B , Maccario , L , Häggblom , M , Vogel , T , Larose , C & Bromberg , Y 2020 , ' Snow microbiome functional analyses reveal novel aspects of microbial metabolism of complex organic compounds ' , MicrobiologyOpen , vol. 9 , no. 9 , e1100 . https://doi.org/10.1002/mbo3.1100 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/mbo3.1100 |
container_title |
MicrobiologyOpen |
container_volume |
9 |
container_issue |
9 |
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1797570896937877504 |