The role of volume-sensitive ion transport systems in regulation of epithelial transport
Udgivelsesdato: 2007-Sep This review focuses on using the knowledge on volume-sensitive transport systems in Ehrlich ascites tumour cells and NIH-3T3 cells to elucidate osmotic regulation of salt transport in epithelia. Using the intestine of the European eel (Anguilla anguilla) (an absorptive epith...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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ftcopenhagenunip:oai:pure.atira.dk:publications/26853340-0cae-11de-8478-000ea68e967b 2023-05-15T13:27:48+02:00 The role of volume-sensitive ion transport systems in regulation of epithelial transport Hoffmann, Else Kay Schettino, T Marshall, W S 2007 https://curis.ku.dk/portal/da/publications/the-role-of-volumesensitive-ion-transport-systems-in-regulation-of-epithelial-transport(26853340-0cae-11de-8478-000ea68e967b).html https://doi.org/10.1016/j.cbpa.2006.11.023 eng eng info:eu-repo/semantics/restrictedAccess Hoffmann , E K , Schettino , T & Marshall , W S 2007 , ' The role of volume-sensitive ion transport systems in regulation of epithelial transport ' , Comparative Biochemistry and Physiology A , vol. 148 , no. 1 , pp. 29-43 . https://doi.org/10.1016/j.cbpa.2006.11.023 article 2007 ftcopenhagenunip https://doi.org/10.1016/j.cbpa.2006.11.023 2022-02-24T00:01:43Z Udgivelsesdato: 2007-Sep This review focuses on using the knowledge on volume-sensitive transport systems in Ehrlich ascites tumour cells and NIH-3T3 cells to elucidate osmotic regulation of salt transport in epithelia. Using the intestine of the European eel (Anguilla anguilla) (an absorptive epithelium of the type described in the renal cortex thick ascending limb (cTAL)) we have focused on the role of swelling-activated K+- and anion-conductive pathways in response to hypotonicity, and on the role of the apical (luminal) Na+-K+-2Cl- cotransporter (NKCC2) in the response to hypertonicity. The shrinkage-induced activation of NKCC2 involves an interaction between the cytoskeleton and protein phosphorylation events via PKC and myosin light chain kinase (MLCK). Killifish (Fundulus heteroclitus) opercular epithelium is a Cl(-)-secreting epithelium of the type described in exocrine glands, having a CFTR channel on the apical side and the Na+/K+ ATPase, NKCC1 and a K+ channel on the basolateral side. Osmotic control of Cl- secretion across the operculum epithelium includes: (i) hyperosmotic shrinkage activation of NKCC1 via PKC, MLCK, p38, OSR1 and SPAK; (ii) deactivation of NKCC by hypotonic cell swelling and a protein phosphatase, and (iii) a protein tyrosine kinase acting on the focal adhesion kinase (FAK) to set levels of NKCC activity. Article in Journal/Newspaper Anguilla anguilla University of Copenhagen: Research Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 148 1 29 43 |
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University of Copenhagen: Research |
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ftcopenhagenunip |
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English |
description |
Udgivelsesdato: 2007-Sep This review focuses on using the knowledge on volume-sensitive transport systems in Ehrlich ascites tumour cells and NIH-3T3 cells to elucidate osmotic regulation of salt transport in epithelia. Using the intestine of the European eel (Anguilla anguilla) (an absorptive epithelium of the type described in the renal cortex thick ascending limb (cTAL)) we have focused on the role of swelling-activated K+- and anion-conductive pathways in response to hypotonicity, and on the role of the apical (luminal) Na+-K+-2Cl- cotransporter (NKCC2) in the response to hypertonicity. The shrinkage-induced activation of NKCC2 involves an interaction between the cytoskeleton and protein phosphorylation events via PKC and myosin light chain kinase (MLCK). Killifish (Fundulus heteroclitus) opercular epithelium is a Cl(-)-secreting epithelium of the type described in exocrine glands, having a CFTR channel on the apical side and the Na+/K+ ATPase, NKCC1 and a K+ channel on the basolateral side. Osmotic control of Cl- secretion across the operculum epithelium includes: (i) hyperosmotic shrinkage activation of NKCC1 via PKC, MLCK, p38, OSR1 and SPAK; (ii) deactivation of NKCC by hypotonic cell swelling and a protein phosphatase, and (iii) a protein tyrosine kinase acting on the focal adhesion kinase (FAK) to set levels of NKCC activity. |
format |
Article in Journal/Newspaper |
author |
Hoffmann, Else Kay Schettino, T Marshall, W S |
spellingShingle |
Hoffmann, Else Kay Schettino, T Marshall, W S The role of volume-sensitive ion transport systems in regulation of epithelial transport |
author_facet |
Hoffmann, Else Kay Schettino, T Marshall, W S |
author_sort |
Hoffmann, Else Kay |
title |
The role of volume-sensitive ion transport systems in regulation of epithelial transport |
title_short |
The role of volume-sensitive ion transport systems in regulation of epithelial transport |
title_full |
The role of volume-sensitive ion transport systems in regulation of epithelial transport |
title_fullStr |
The role of volume-sensitive ion transport systems in regulation of epithelial transport |
title_full_unstemmed |
The role of volume-sensitive ion transport systems in regulation of epithelial transport |
title_sort |
role of volume-sensitive ion transport systems in regulation of epithelial transport |
publishDate |
2007 |
url |
https://curis.ku.dk/portal/da/publications/the-role-of-volumesensitive-ion-transport-systems-in-regulation-of-epithelial-transport(26853340-0cae-11de-8478-000ea68e967b).html https://doi.org/10.1016/j.cbpa.2006.11.023 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Hoffmann , E K , Schettino , T & Marshall , W S 2007 , ' The role of volume-sensitive ion transport systems in regulation of epithelial transport ' , Comparative Biochemistry and Physiology A , vol. 148 , no. 1 , pp. 29-43 . https://doi.org/10.1016/j.cbpa.2006.11.023 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cbpa.2006.11.023 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
148 |
container_issue |
1 |
container_start_page |
29 |
op_container_end_page |
43 |
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